<
Western Blot (WB)
(Western Blot: Sample: Rat Serum.)
Rabbit anti-Rat Glutamate Decarboxylase 2, Acid (GAD2) Polyclonal Antibody | anti-GAD2 antibody
Polyclonal Antibody to Glutamate Decarboxylase 2, Acid (GAD2)
Gene Names
Gad2; gad65
Reactivity
Rat
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Glutamate Decarboxylase 2; Acid (GAD2); Polyclonal Antibody; Polyclonal Antibody to Glutamate Decarboxylase 2; anti-GAD2 antibody
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against GAD2. It has been selected for its ability to recognize GAD2 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSGSEF-MVG LAADWLTSTA NTNMFTYEIA PVFVLLEYVT LKKMREIIGW PGGSGDGIFS PGGAISNMYA MLIARYKMFP EVKEKGMAAV PRLIAFTSEH SHFSLKKGAA ALGIGTDSVI LIKCDERGKM IPSDLERRIL EVKQKGFVPF LVSATAGTTV YGAFDPLLAV ADICKKYKIW MHVDAAWGGG LLMS
MGHHHHHHSGSEF-MVG LAADWLTSTA NTNMFTYEIA PVFVLLEYVT LKKMREIIGW PGGSGDGIFS PGGAISNMYA MLIARYKMFP EVKEKGMAAV PRLIAFTSEH SHFSLKKGAA ALGIGTDSVI LIKCDERGKM IPSDLERRIL EVKQKGFVPF LVSATAGTTV YGAFDPLLAV ADICKKYKIW MHVDAAWGGG LLMS
Sequence Length
585
Applicable Applications for anti-GAD2 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant GAD2 (Met188~Ser374) expressed in E.coli.
Cross Reactivity
Rat
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2053261
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Rat Serum.)
Western Blot (WB)
(Western Blot: Sample: Rat Serum.)
Western Blot (WB)
(Western Blot: Sample: Recombinant GAD2, Rat.)
Western Blot (WB)
(Western Blot: Sample: Recombinant GAD2, Rat.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Stomach Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Stomach Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Heart Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Heart Tissue.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
65,402 Da
NCBI Official Full Name
glutamate decarboxylase 2
NCBI Official Synonym Full Names
glutamate decarboxylase 2
NCBI Official Symbol
Gad2
NCBI Official Synonym Symbols
gad65
NCBI Protein Information
glutamate decarboxylase 2
UniProt Protein Name
Glutamate decarboxylase 2
Protein Family
UniProt Gene Name
Gad2
UniProt Synonym Gene Names
Gad65; GAD-65
NCBI Description
catalyzes synthesis of gamma-Aminobutyric acid (GABA), the most widely distributed inhibitory neurotransmitter [RGD, Feb 2006]
Uniprot Description
GAD2: Catalyzes the production of GABA. Homodimer. Belongs to the group II decarboxylase family.
Protein type: Amino Acid Metabolism - alanine, aspartate and glutamate; Carbohydrate Metabolism - butanoate; EC 4.1.1.15; Lyase; Other Amino Acids Metabolism - beta-alanine; Other Amino Acids Metabolism - taurine and hypotaurine
Chromosomal Location of Human Ortholog: 17q12.3
Cellular Component: axon; cell junction; cytoplasm; cytoplasmic vesicle; cytosol; Golgi membrane; perinuclear region of cytoplasm; presynaptic membrane; synapse; synaptic vesicle membrane
Molecular Function: glutamate binding; glutamate decarboxylase activity; protein heterodimerization activity; pyridoxal phosphate binding
Biological Process: glutamate decarboxylation to succinate; neurotransmitter biosynthetic process; response to drug; synaptic transmission
Research Articles on GAD2
Similar Products
Product Notes
The GAD2 gad2 (Catalog #AAA2001977) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Glutamate Decarboxylase 2, Acid (GAD2) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Glutamate Decarboxylase 2, Acid (GAD2) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the GAD2 gad2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG SEF-MVG LAADWLTSTA NTNMFTYEIA PVFVLLEYVT LKKMREIIGW PGGSGDGIFS PGGAISNMYA MLIARYKMFP EVKEKGMAAV PRLIAFTSEH SHFSLKKGAA ALGIGTDSVI LIKCDERGKM IPSDLERRIL EVKQKGFVPF LVSATAGTTV YGAFDPLLAV ADICKKYKIW MHVDAAWGGG LLMS. It is sometimes possible for the material contained within the vial of "Glutamate Decarboxylase 2, Acid (GAD2), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
