Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Human Plexin B1 (PLXNB1) Polyclonal Antibody | anti-PLXNB1 antibody
Polyclonal Antibody to Plexin B1 (PLXNB1)
Gene Names
PLXNB1; SEP; PLXN5; PLEXIN-B1
Reactivity
Human
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Plexin B1 (PLXNB1); Polyclonal Antibody; Polyclonal Antibody to Plexin B1 (PLXNB1); anti-PLXNB1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against PLXNB1. It has been selected for its ability to recognize PLXNB1 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-A KEKMLDQLYK GVPLTQRPDP RTLDVEWRSG VAGHLILSDE DVTSEVQGLW RRLNTLQHYK VPDGATVALV PCLTKHVLRE NQDYVPGERT PMLEDVDEGG IRPWHLVKPS DEPEPPRPRR GSLRGGERER AKAIPEIYLT RLLSMKGTLQ KFVDDLFQVI LSTSRPVPLA VKYFFDLLDE QAQQHGISDQ DTIHIWKTNS LPLRFWINII KNPQFVFDVQ TSDNMDAVLL VIAQTFMDAC TLADHKLGRD SPINKLLYAR DI
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-A KEKMLDQLYK GVPLTQRPDP RTLDVEWRSG VAGHLILSDE DVTSEVQGLW RRLNTLQHYK VPDGATVALV PCLTKHVLRE NQDYVPGERT PMLEDVDEGG IRPWHLVKPS DEPEPPRPRR GSLRGGERER AKAIPEIYLT RLLSMKGTLQ KFVDDLFQVI LSTSRPVPLA VKYFFDLLDE QAQQHGISDQ DTIHIWKTNS LPLRFWINII KNPQFVFDVQ TSDNMDAVLL VIAQTFMDAC TLADHKLGRD SPINKLLYAR DI
Sequence Length
2135
Applicable Applications for anti-PLXNB1 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant PLXNB1 (Ala1780~Ile2042) expressed in E.coli.
Cross Reactivity
Human
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2079684
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DABstainingonIHC-P.Samples:HumanTissue))
Immunohistochemistry (IHC)
(DABstainingonIHC-P.Samples:HumanTissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
78,395 Da
NCBI Official Full Name
plexin-B1
NCBI Official Synonym Full Names
plexin B1
NCBI Official Symbol
PLXNB1
NCBI Official Synonym Symbols
SEP; PLXN5; PLEXIN-B1
NCBI Protein Information
plexin-B1
UniProt Protein Name
Plexin-B1
Protein Family
UniProt Gene Name
PLXNB1
UniProt Synonym Gene Names
KIAA0407; PLXN5; SEP
Uniprot Description
PLXNB1: Receptor for SEMA4D. Plays a role in RHOA activation and subsequent changes of the actin cytoskeleton. Plays a role in axon guidance, invasive growth and cell migration. Belongs to the plexin family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; Receptor, misc.
Chromosomal Location of Human Ortholog: 3p21.31
Cellular Component: extracellular region; integral component of plasma membrane; intracellular; plasma membrane
Molecular Function: GTPase activating protein binding; GTPase activator activity; protein binding; receptor activity; semaphorin receptor activity; semaphorin receptor binding; transmembrane receptor activity
Biological Process: axon extension; cell migration; negative regulation of cell adhesion; negative regulation of osteoblast proliferation; neuron projection morphogenesis; positive regulation of axonogenesis; positive regulation of GTPase activity; positive regulation of phosphoinositide 3-kinase cascade; regulation of cell shape; regulation of cytoskeleton organization; regulation of GTPase activity; signal transduction
Research Articles on PLXNB1
Similar Products
Product Notes
The PLXNB1 plxnb1 (Catalog #AAA2001951) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Plexin B1 (PLXNB1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Plexin B1 (PLXNB1) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the PLXNB1 plxnb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-A KEKMLDQLYK GVPLTQRPDP RTLDVEWRSG VAGHLILSDE DVTSEVQGLW RRLNTLQHYK VPDGATVALV PCLTKHVLRE NQDYVPGERT PMLEDVDEGG IRPWHLVKPS DEPEPPRPRR GSLRGGERER AKAIPEIYLT RLLSMKGTLQ KFVDDLFQVI LSTSRPVPLA VKYFFDLLDE QAQQHGISDQ DTIHIWKTNS LPLRFWINII KNPQFVFDVQ TSDNMDAVLL VIAQTFMDAC TLADHKLGRD SPINKLLYAR DI. It is sometimes possible for the material contained within the vial of "Plexin B1 (PLXNB1), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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