Mouse Tumor Protein, Translationally Controlled 1 (TPT1) CLIA Kit | TPT1 clia kit

Mouse Tumor Protein, Translationally Controlled 1 (TPT1) CLIA Kit

Cat. Num. AAA2001115

• Gene Names: TPT1; HRF; p02; p23; TCTP
• Reactivity: Mouse
• Synonyms: Tumor Protein; Translationally Controlled 1 (TPT1); Mouse Tumor Protein; Translationally Controlled 1 (TPT1) CLIA Kit; HRF; TCTP; p02; p23; Fortilin; Translationally-Controlled Tumor Protein; Histamine-releasing factor; TPT1 clia kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Tumor Protein, Translationally Controlled 1 (TPT1).; No significant cross-reactivity or interference between Tumor Protein, Translationally Controlled 1 (TPT1) and analogues was observed.
• Sequence Length: 172

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates and Other Biological Fluids
• Detection Range: 27.4-20,000pg/mL
• Sensitivity: < 10.3pg/mL
• Application: Chemiluminescent immunoassay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tumor Protein, Translationally Controlled 1 (TPT1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tumor Protein, Translationally Controlled 1 (TPT1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TPT1 clia kit

The microplate provided in this kit has been pre-coated with an antibody specific to Tumor Protein, Translationally Controlled 1 (TPT1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Tumor Protein, Translationally Controlled 1 (TPT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Tumor Protein, Translationally Controlled 1 (TPT1) level in the sample or standard.;

Product Categories/Family for TPT1 clia kit

Tumor immunity

NCBI and Uniprot Product Information

• NCBI GI #: 13097159
• NCBI GeneID: 7178
• UniProt Accession #: P13693; Q6YLS2; Q7Z4J4; Q8TBK7; Q96EE2; Q9UC70; B2R7E5
• Molecular Weight: 15,597 Da
• NCBI Official Full Name: Tumor protein, translationally-controlled 1
• NCBI Official Synonym Full Names: tumor protein, translationally-controlled 1
• NCBI Official Symbol: TPT1
• NCBI Official Synonym Symbols: HRF; p02; p23; TCTP
• NCBI Protein Information: translationally-controlled tumor protein
• UniProt Protein Name: Translationally-controlled tumor protein
• Protein Family: tRNA 2'-phosphotransferase
• UniProt Gene Name: TPT1
• UniProt Synonym Gene Names: TCTP; HRF
• UniProt Entry Name: TCTP_HUMAN

Uniprot Description

TPT1: a novel heat shock protein with antiapoptotic and chaperone-like activity. Its levels are highly regulated in response to various cellular stimuli and stresses. Downregulated in response to proapoptotic treatments that induce Ca(++) stress, in a PKR-dependent manner. Colocalizes with CHFR to the mitotic spindle. Appears to be involved in calcium binding and microtubule stabilization. Its exosomal secretion is induced following DNA damage. Interacts with MCL1 and STEAP3, a p53-inducible transmembrane protein that facilitates the exosomal secretion of TPT1. Secreted TPT1 acts as a histamine-releasing factor, participates in inflammatory responses by inducing the release of histamine, and may play a role in tumor reversion. May play a direct role in placental calcium transport. Phosphorylation by Plk may decrease its microtubule-stabilizing activity and promote microtubule dynamics following metaphase. Critical for peripheral T cell maintenance and TCR-mediated cell proliferation.

Protein type: Microtubule-binding; Calcium-binding

Chromosomal Location of Human Ortholog: 13q14

Cellular Component: multivesicular body; extracellular space; tubulin complex; cytoplasm; nucleus

Molecular Function: protein binding; calcium ion binding

Biological Process: cellular calcium ion homeostasis; regulation of apoptosis; calcium ion transport; response to virus; negative regulation of apoptosis