Mouse Fibroblast Growth Factor 3 (FGF3) CLIA Kit | FGF3 clia kit

Mouse Fibroblast Growth Factor 3 (FGF3) CLIA Kit

Cat. Num. AAA2001091

• Gene Names: FGF3; INT2; HBGF-3
• Reactivity: Mouse
• Synonyms: Fibroblast Growth Factor 3 (FGF3); Mouse Fibroblast Growth Factor 3 (FGF3) CLIA Kit; HBGF3; INT2; Heparin-binding growth factor 3; Murine Mammary Tumor Virus Integration Site(v-int-2)Oncogene Homolog; INT-2 Proto-Oncogene Protein; Proto-oncogene Int-2; FGF3 clia kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Fibroblast Growth Factor 3 (FGF3).; No significant cross-reactivity or interference between Fibroblast Growth Factor 3 (FGF3) and analogues was observed.
• Sequence Length: 239

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma and Other Biological Fluids
• Detection Range: 1.37-1,000pg/mL
• Sensitivity: < 0.57pg/mL
• Application: Chemiluminescent immunoassay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibroblast Growth Factor 3 (FGF3) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibroblast Growth Factor 3 (FGF3) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FGF3 clia kit

The microplate provided in this kit has been pre-coated with an antibody specific to Fibroblast Growth Factor 3 (FGF3). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Fibroblast Growth Factor 3 (FGF3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Fibroblast Growth Factor 3 (FGF3) level in the sample or standard.;

Product Categories/Family for FGF3 clia kit

Cytokine

NCBI and Uniprot Product Information

• NCBI GI #: 4885233
• NCBI GeneID: 2248
• NCBI Accession #: NP_005238.1
• NCBI GenBank Nucleotide #: NM_005247.2
• UniProt Accession #: P11487; Q0VG69
• Molecular Weight: 26,887 Da
• NCBI Official Full Name: fibroblast growth factor 3
• NCBI Official Synonym Full Names: fibroblast growth factor 3
• NCBI Official Symbol: FGF3
• NCBI Official Synonym Symbols: INT2; HBGF-3
• NCBI Protein Information: fibroblast growth factor 3
• UniProt Protein Name: Fibroblast growth factor 3
• Protein Family: Fibroblast growth factor
• UniProt Gene Name: FGF3
• UniProt Synonym Gene Names: INT2; FGF-3; HBGF-3
• UniProt Entry Name: FGF3_HUMAN

NCBI Description

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This gene was identified by its similarity with mouse fgf3/int-2, a proto-oncogene activated in virally induced mammary tumors in the mouse. Frequent amplification of this gene has been found in human tumors, which may be important for neoplastic transformation and tumor progression. Studies of the similar genes in mouse and chicken suggested the role in inner ear formation. [provided by RefSeq, Jul 2008]

Uniprot Description

FGF3: Plays an important role in the regulation of embryonic development, cell proliferation, and cell differentiation. Required for normal ear development. Defects in FGF3 are a cause of deafness with labyrinthine aplasia, microtia and microdontia (LAMM); also known as congenital deafness with inner ear agenesis, microtia and microdontia. LAMM consists of a unique autosomal recessive syndrome characterized by type I microtia, microdontia, and profound congenital deafness associated with a complete absence of inner ear structures (Michel aplasia). Belongs to the heparin-binding growth factors family.

Protein type: Secreted; Secreted, signal peptide; Oncoprotein

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: Golgi apparatus; extracellular region

Molecular Function: protein binding; growth factor activity; fibroblast growth factor receptor binding

Biological Process: negative regulation of cardiac muscle development; epidermal growth factor receptor signaling pathway; anatomical structure morphogenesis; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; thymus development; nerve growth factor receptor signaling pathway; semicircular canal morphogenesis; signal transduction; cell-cell signaling; positive regulation of cell division; induction of an organ; positive regulation of cell proliferation; insulin receptor signaling pathway; innate immune response; otic vesicle formation

Disease: Deafness, Congenital, With Inner Ear Agenesis, Microtia, And Microdontia