Rat Tyrosine Hydroxylase (TH) CLIA Kit | TH clia kit

Rat Tyrosine Hydroxylase (TH) CLIA Kit

Cat. Num. AAA2000939

• Gene Names: TH; TYH; DYT14; DYT5b
• Reactivity: Rat
• Synonyms: Tyrosine Hydroxylase (TH); Rat Tyrosine Hydroxylase (TH) CLIA Kit; TYH; DYT5b; Tyrosine 3-Monooxygenase; Dystonia 14; TH clia kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Tyrosine Hydroxylase (TH).; No significant cross-reactivity or interference between Tyrosine Hydroxylase (TH) and analogues was observed.
• Sequence Length: 106

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates and Other Biological Fluids
• Detection Range: 13.7-10,000pg/mL
• Sensitivity: < 5.6pg/mL
• Application: Chemiluminescent immunoassay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tyrosine Hydroxylase (TH) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tyrosine Hydroxylase (TH) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TH clia kit

The microplate provided in this kit has been pre-coated with an antibody specific to Tyrosine Hydroxylase (TH). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Tyrosine Hydroxylase (TH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Tyrosine Hydroxylase (TH) level in the sample or standard.;

Product Categories/Family for TH clia kit

Signal transduction; Enzyme & Kinase; Neuroscience; Hormone metabolism

NCBI and Uniprot Product Information

• NCBI GI #: 986909
• NCBI GeneID: 7054
• UniProt Accession #: P07101; Q0PWM2; Q0PWM3; Q15585; Q15588; Q15589; Q2M3B4; B7ZL70; B7ZL73
• Molecular Weight: 44,898 Da
• NCBI Official Full Name: tyrosine hydroxylase, partial
• NCBI Official Synonym Full Names: tyrosine hydroxylase
• NCBI Official Symbol: TH
• NCBI Official Synonym Symbols: TYH; DYT14; DYT5b
• NCBI Protein Information: tyrosine 3-monooxygenase
• UniProt Protein Name: Tyrosine 3-monooxygenase
• UniProt Gene Name: TH
• UniProt Synonym Gene Names: TYH; TH
• UniProt Entry Name: TY3H_HUMAN

NCBI Description

The protein encoded by this gene is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons. Mutations in this gene have been associated with autosomal recessive Segawa syndrome. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

TH: an enzyme involved in the conversion of phenylalanine to dopamine. As the rate-limiting enzyme in the synthesis of catecholamines, tyrosine hydroxylase has a key role in the physiology of adrenergic neurons. Four splice variant isoforms have been described.

Protein type: Endoplasmic reticulum; Vesicle; Mitochondrial; Amino Acid Metabolism - tyrosine; Oxidoreductase; EC 1.14.16.2

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: internal side of plasma membrane; neuron projection; smooth endoplasmic reticulum; mitochondrion; dendrite; melanosome membrane; terminal button; perikaryon; cytosol; synaptic vesicle; cytoplasm; cytoplasmic vesicle; nucleus

Molecular Function: protein domain specific binding; amino acid binding; protein binding; enzyme binding; ferric iron binding; ferrous iron binding; dopamine binding; oxygen binding; tyrosine 3-monooxygenase activity

Biological Process: heart morphogenesis; heart development; response to lipopolysaccharide; dopamine biosynthetic process; phytoalexin metabolic process; norepinephrine biosynthetic process; dopamine biosynthetic process from tyrosine; catecholamine biosynthetic process; response to electrical stimulus; epinephrine biosynthetic process; response to corticosterone stimulus; neurotransmitter biosynthetic process; response to pyrethroid; response to light stimulus; anatomical structure morphogenesis; phthalate metabolic process; mating behavior; social behavior; organ morphogenesis; eye photoreceptor cell development; circadian sleep/wake cycle; response to ethanol; response to zinc ion; cerebral cortex development; response to activity; response to water deprivation; response to peptide hormone stimulus; synaptic transmission, dopaminergic; locomotory behavior; fatty acid metabolic process; regulation of heart contraction; response to estradiol stimulus; response to salt stress; sensory perception of sound; visual perception; glycoside metabolic process; response to nutrient levels; terpene metabolic process; sphingolipid metabolic process; eating behavior; response to amphetamine; multicellular organismal aging; isoquinoline alkaloid metabolic process; learning; response to herbicide; response to ether; synaptic vesicle amine transport; memory; pigmentation; response to hypoxia; embryonic camera-type eye morphogenesis

Disease: Segawa Syndrome, Autosomal Recessive