Western Blot (WB)
(Cell lysates of HepG2 (40ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PDCD4 (1:2,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Mouse anti-Human PDCD4 Monoclonal Antibody | anti-PDCD4 antibody
PDCD4 antibody
Gene Names
PDCD4; H731
Reactivity
Human
Applications
ELISA, Western Blot
Purity
By protein-G affinity chromatography
Synonyms
PDCD4; Monoclonal Antibody; PDCD4 antibody; Programmed cell death 4 isoform 1; neoplastic transformation inhibitor; H731; MGC33046; MGC33047; PDPC4; programmed cell death 4 (neoplastic transformation inhibitor); nuclear antigen H731; anti-PDCD4 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1, k
Clone Number
k4C1
Purity/Purification
By protein-G affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
469
Applicable Applications for anti-PDCD4 antibody
ELISA (EIA), Western Blot (WB) (Cell lysate)
Application Notes
WB: 1:1,000~ 3,000.
Recommended starting dilution is 1:2,000.
Recommended starting dilution is 1:2,000.
Antigen Species
Human
Immunogen
Recombinant human PDCD4 (1-469aa) purified from E. coli
Clone Note
Anti-human PDCD4 mAb, clone k4C1, is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human PDCD4 protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Western Blot (WB)
(Cell lysates of HepG2 (40ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PDCD4 (1:2,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Western Blot (WB)
(Cell lysates of HepG2 (40ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PDCD4 (1:2,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Related Product Information for anti-PDCD4 antibody
Programmed cell death 4 (PDCD4) encodes a tumor suppressor protein whose expression is lost in progressed carcinomas of lung, breast, colon, and prostate. The expression of PDCD4 gene is strongly induced during apoptosis in a number of cell types. It is modulated by cytokines in natural killer and T cells.
Product Categories/Family for anti-PDCD4 antibody
References
Palamarchuk A, et al., (2006) Cancer Res 65 (24):11282-11286; Yoshina, et al., (1999) Pathol Int 49 (12):1067-1077
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
50,576 Da
NCBI Official Full Name
programmed cell death protein 4 isoform 1
NCBI Official Synonym Full Names
programmed cell death 4
NCBI Official Symbol
PDCD4
NCBI Official Synonym Symbols
H731
NCBI Protein Information
programmed cell death protein 4
UniProt Protein Name
Programmed cell death protein 4
Protein Family
UniProt Gene Name
PDCD4
UniProt Synonym Gene Names
H731
NCBI Description
This gene is a tumor suppressor and encodes a protein that binds to the eukaryotic translation initiation factor 4A1 and inhibits its function by preventing RNA binding. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2010]
Uniprot Description
Inhibits translation initiation and cap-dependent translation. May excert its function by hindering the interaction between EIF4A1 and EIF4G. Inhibits the helicase activity of EIF4A. Modulates the activation of JUN kinase. Down-regulates the expression of MAP4K1, thus inhibiting events important in driving invasion, namely, MAPK85 activation and consequent JUN-dependent transcription. May play a role in apoptosis. Tumor suppressor. Inhibits tumor promoter-induced neoplastic transformation. Binds RNA ().
Research Articles on PDCD4
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Product Notes
The PDCD4 pdcd4 (Catalog #AAA200081) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The PDCD4 antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PDCD4 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB) (Cell lysate). WB: 1:1,000~ 3,000. Recommended starting dilution is 1:2,000. Researchers should empirically determine the suitability of the PDCD4 pdcd4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PDCD4, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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