Transglutaminase 2, Tissue (TGM2) CLIA Kit | TGM2 clia kit

CLIA Kit for Transglutaminase 2, Tissue (TGM2)

Cat. Num. AAA2000529

• Gene Names: TGM2; TG2; TGC; GNAH; HEL-S-45; G-ALPHA-h
• Synonyms: Transglutaminase 2; Tissue (TGM2); CLIA Kit for Transglutaminase 2; TGM2 clia kit

• Specificity: This assay has high sensitivity and excellent specificity for detection of TGM2. No significant cross-reactivity or interference between TGM2 and analogues was observed.
• Sequence Length: 687

• Intended Uses: The Chemiluminescent Immunoaassay kit is designed for the in vitro sensitive quantitative measurement of TGM2 in mouse serum, plasma, tissue homogenates and other biological fluids.
• Detection Range: 0.05-40ng/mL. The standard curve concentrations used for the CLIA’s were 40ng/mL, 13.33ng/mL, 4.44ng/mL, 1.48ng/mL, 0.49ng/mL, 0.16ng/mL, 0.05ng/mL.
• Sensitivity: The minimum detectable dose of TGM2 is typically less than 0.01 ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean RLU value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TGM2 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TGM2 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TGM2 clia kit

Principle of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to TGM2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to TGM2 . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the TGM2 level in the sample or standard.

NCBI and Uniprot Product Information

• NCBI GI #: 50593093
• NCBI GeneID: 7052
• UniProt Accession #: P21980; Q16436; Q6B838; Q9BTN7; Q9UH35; E1P5V9
• Molecular Weight: 77,329 Da
• NCBI Official Full Name: transglutaminase 2
• NCBI Official Synonym Full Names: transglutaminase 2
• NCBI Official Symbol: TGM2
• NCBI Official Synonym Symbols: TG2; TGC; GNAH; HEL-S-45; G-ALPHA-h
• NCBI Protein Information: protein-glutamine gamma-glutamyltransferase 2; TG(C); TGase C; TGase H; TGase-2; TGase-H; transglutaminase C; transglutaminase H; transglutaminase-2; tissue transglutaminase; epididymis secretory protein Li 45; protein-glutamine-gamma-glutamyltransferase; C polypeptide, protein-glutamine-gamma-glutamyltransferase
• UniProt Protein Name: Protein-glutamine gamma-glutamyltransferase 2
• Protein Family: Protein-glutamine gamma-glutamyltransferase
• UniProt Gene Name: TGM2
• UniProt Synonym Gene Names: TG(C); TGC; TGase C; TGase H; TGase-2
• UniProt Entry Name: TGM2_HUMAN

NCBI Description

Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. Finally, the encoded protein is the autoantigen implicated in celiac disease. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

TGM2: an enzyme of the transglutaminase family that catalyzes the crosslinking of proteins and the conjugation of polyamines to proteins. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. . Is the autoantigen in coeliac disease and plays a role in apoptosis, cellular differentiation and matrix stabilisation. Three alternatively spliced isoforms have been described.

Protein type: EC 2.3.2.13; Transferase

Chromosomal Location of Human Ortholog: 20q12

Cellular Component: focal adhesion; mitochondrion; plasma membrane; cytosol

Molecular Function: protein domain specific binding; protein binding; GTP binding; protein-glutamine gamma-glutamyltransferase activity; metal ion binding

Biological Process: elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of cell adhesion; isopeptide cross-linking via N6-(L-isoglutamyl)-L-lysine; positive regulation of apoptosis; positive regulation of smooth muscle cell proliferation; reduction of endoplasmic reticulum calcium ion concentration; blood vessel remodeling; apoptotic cell clearance; protein homooligomerization; elevation of mitochondrial calcium ion concentration; positive regulation of inflammatory response