Human Alpha-Methylacyl Coenzyme A Racemase (aMACR) ELISA Kit | aMACR elisa kit

Human Alpha-Methylacyl Coenzyme A Racemase (aMACR) ELISA Kit

Cat. Num. AAA2000355

• Gene Names: AMACR; RM; RACE; CBAS4; AMACRD
• Reactivity: Human
• Synonyms: Alpha-Methylacyl Coenzyme A Racemase (aMACR); Human Alpha-Methylacyl Coenzyme A Racemase (aMACR) ELISA Kit; RACE; 2-methylacyl-CoA racemase; aMACR elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Alpha-Methylacyl Coenzyme A Racemase (aMACR).; No significant cross-reactivity or interference between Alpha-Methylacyl Coenzyme A Racemase (aMACR) and analogues was observed.
• Sequence Length: 394

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.066ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Alpha-Methylacyl Coenzyme A Racemase (aMACR) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Alpha-Methylacyl Coenzyme A Racemase (aMACR) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for aMACR elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-Methylacyl Coenzyme A Racemase (aMACR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alpha-Methylacyl Coenzyme A Racemase (aMACR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alpha-Methylacyl Coenzyme A Racemase (aMACR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alpha-Methylacyl Coenzyme A Racemase (aMACR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for aMACR elisa kit

Enzyme & Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 266456254
• NCBI GeneID: 23600
• NCBI Accession #: NP_001161067.1
• NCBI GenBank Nucleotide #: NM_001167595.1
• UniProt Accession #: Q9UHK6; O43673; Q3KT79; Q96GH1; Q9Y3Q1; A5YM47; B8Y916; B8Y918; F8W9N1
• Molecular Weight: 43,860 Da
• NCBI Official Full Name: alpha-methylacyl-CoA racemase isoform 3
• NCBI Official Synonym Full Names: alpha-methylacyl-CoA racemase
• NCBI Official Symbol: AMACR
• NCBI Official Synonym Symbols: RM; RACE; CBAS4; AMACRD
• NCBI Protein Information: alpha-methylacyl-CoA racemase
• UniProt Protein Name: Alpha-methylacyl-CoA racemase
• Protein Family: Alpha-methylacyl-CoA racemase
• UniProt Gene Name: AMACR
• UniProt Entry Name: AMACR_HUMAN

NCBI Description

This gene encodes a racemase. The encoded enzyme interconverts pristanoyl-CoA and C27-bile acylCoAs between their (R)- and (S)-stereoisomers. The conversion to the (S)-stereoisomers is necessary for degradation of these substrates by peroxisomal beta-oxidation. Encoded proteins from this locus localize to both mitochondria and peroxisomes. Mutations in this gene may be associated with adult-onset sensorimotor neuropathy, pigmentary retinopathy, and adrenomyeloneuropathy due to defects in bile acid synthesis. Alternatively spliced transcript variants have been described. Read-through transcription also exists between this gene and the upstream neighboring C1QTNF3 (C1q and tumor necrosis factor related protein 3) gene. [provided by RefSeq, Mar 2011]

Uniprot Description

AMACR: Racemization of 2-methyl-branched fatty acid CoA esters. Responsible for the conversion of pristanoyl-CoA and C27-bile acyl-CoAs to their (S)-stereoisomers. Belongs to the CaiB/BaiF CoA-transferase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial; EC 5.1.99.4; Lipid Metabolism - primary bile acid biosynthesis; Isomerase

Chromosomal Location of Human Ortholog: 5p13

Cellular Component: peroxisomal matrix; mitochondrion; cytoplasm; peroxisome

Molecular Function: receptor binding; alpha-methylacyl-CoA racemase activity

Biological Process: bile acid biosynthetic process; fatty acid beta-oxidation using acyl-CoA oxidase; bile acid metabolic process; cellular lipid metabolic process

Disease: Bile Acid Synthesis Defect, Congenital, 4; Alpha-methylacyl-coa Racemase Deficiency