Human Beta-Carotene-15,15'-Monooxygenase 1 (bCMO1) ELISA Kit | bCMO1 elisa kit

Human Beta-Carotene-15,15'-Monooxygenase 1 (bCMO1) ELISA Kit

Cat. Num. AAA2000326

• Gene Names: BCO1; BCO; BCDO; BCMO; BCDO1; BCMO1
• Reactivity: Human
• Synonyms: Beta-Carotene-15; 15'-Monooxygenase 1 (bCMO1); Human Beta-Carotene-15; 15'-Monooxygenase 1 (bCMO1) ELISA Kit; BCO1; BCDO; BCDO1; BCMO; Beta-carotene dioxygenase 1; Beta-carotene oxygenase 1; Beta; beta-carotene 15; 15'-monooxygenase; bCMO1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Beta-Carotene-15,15'-Monooxygenase 1 (bCMO1).; No significant cross-reactivity or interference between Beta-Carotene-15,15'-Monooxygenase 1 (bCMO1) and analogues was observed.
• Sequence Length: 547

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 1.25-20ng/mL
• Sensitivity: < 0.49ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Beta-Carotene-15,15'-Monooxygenase 1 (bCMO1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Beta-Carotene-15,15'-Monooxygenase 1 (bCMO1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for bCMO1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Beta-Carotene-15,15'-Monooxygenase 1 (bCMO1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Beta-Carotene-15,15'-Monooxygenase 1 (bCMO1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Beta-Carotene-15,15'-Monooxygenase 1 (bCMO1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Beta-Carotene-15,15'-Monooxygenase 1 (bCMO1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for bCMO1 elisa kit

Enzyme & Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 116496983
• NCBI GeneID: 53630
• UniProt Accession #: Q9HAY6; Q9NVH5; A0AV48; A0AV50
• Molecular Weight: 62,637 Da
• NCBI Official Full Name: Beta-carotene 15,15'-monooxygenase 1
• NCBI Official Synonym Full Names: beta-carotene oxygenase 1
• NCBI Official Symbol: BCO1
• NCBI Official Synonym Symbols: BCO; BCDO; BCMO; BCDO1; BCMO1
• NCBI Protein Information: beta,beta-carotene 15,15'-monooxygenase
• UniProt Protein Name: Beta,beta-carotene 15,15'-monooxygenase
• UniProt Gene Name: BCO1
• UniProt Entry Name: BCDO1_HUMAN

NCBI Description

Vitamin A metabolism is important for vital processes such as vision, embryonic development, cell differentiation, and membrane and skin protection. The protein encoded by this gene is a key enzyme in beta-carotene metabolism to vitamin A. It catalyzes the oxidative cleavage of beta,beta-carotene into two retinal molecules. [provided by RefSeq, Jul 2008]

Uniprot Description

BCMO1: Symmetrically cleaves beta-carotene into two molecules of retinal. The reaction proceeds in three stages, epoxidation of the 15,15'-double bond, hydration of the double bond leading to ring opening, and oxidative cleavage of the diol formed. Defects in BCMO1 are the cause of autosomal dominant hypercarotenemia and vitamin A deficiency (ADHVAD). Vitamin A is essential for normal embryonic development as well as normal physiological functions in children and adults. Hypercarotenemia is characterized by an excess carotene in the serum, but unlike excess vitamin A, carotene is non-toxic. So far, only a few cases of excess vitamin A have been reported. Individuals were thought to be vitamin A deficient due to an impairment in the conversion of carotenoids to retinal in the intestine. Belongs to the carotenoid oxygenase family.

Protein type: Cofactor and Vitamin Metabolism - retinol; EC 1.14.99.36; Oxidoreductase

Chromosomal Location of Human Ortholog: 16q23.2

Cellular Component: cytosol

Molecular Function: beta-carotene 15,15'-monooxygenase activity; metal ion binding; monooxygenase activity

Biological Process: phototransduction, visible light; vitamin A biosynthetic process; retinal metabolic process; retinol metabolic process; retinoid metabolic process

Disease: Hypercarotenemia And Vitamin A Deficiency, Autosomal Dominant