Spleen Focus Forming Virus Proviral Integration Oncogene (SPI1) ELISA Kit | SPI1 elisa kit

Rat Spleen Focus Forming Virus Proviral Integration Oncogene (SPI1) ELISA Kit

Cat. Num. AAA2000287

• Gene Names: SPI1; OF; PU.1; SFPI1; SPI-1; SPI-A
• Reactivity: Rat
• Synonyms: Spleen Focus Forming Virus Proviral Integration Oncogene (SPI1); Rat Spleen Focus Forming Virus Proviral Integration Oncogene (SPI1) ELISA Kit; OF; PU.1; SFPI1; SPI-1; SPI-A; SPI-1 Proto-Oncogene; Hematopoietic Transcription Factor PU.1; 31 kDa Transforming Protein; SPI1 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Spleen Focus Forming Virus Proviral Integration Oncogene (SPI1).; No significant cross-reactivity or interference between Spleen Focus Forming Virus Proviral Integration Oncogene (SPI1) and analogues was observed.
• Sequence Length: 147

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.116ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Spleen Focus Forming Virus Proviral Integration Oncogene (SPI1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Spleen Focus Forming Virus Proviral Integration Oncogene (SPI1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SPI1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Spleen Focus Forming Virus Proviral Integration Oncogene (SPI1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Spleen Focus Forming Virus Proviral Integration Oncogene (SPI1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Spleen Focus Forming Virus Proviral Integration Oncogene (SPI1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Spleen Focus Forming Virus Proviral Integration Oncogene (SPI1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for SPI1 elisa kit

Tumor immunity

NCBI and Uniprot Product Information

• NCBI GI #: 119588332
• NCBI GeneID: 6688
• UniProt Accession #: P17947
• Molecular Weight: 31,211 Da
• NCBI Official Full Name: spleen focus forming virus (SFFV) proviral integration oncogene spi1, isoform CRA_c, partial
• NCBI Official Synonym Full Names: Spi-1 proto-oncogene
• NCBI Official Symbol: SPI1
• NCBI Official Synonym Symbols: OF; PU.1; SFPI1; SPI-1; SPI-A
• NCBI Protein Information: transcription factor PU.1
• UniProt Protein Name: Transcription factor PU.1
• Protein Family: Transcription factor
• UniProt Gene Name: SPI1
• UniProt Entry Name: SPI1_HUMAN

NCBI Description

This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

PU.1: Binds to the PU-box, a purine-rich DNA sequence (5'- GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B- cells. Also binds RNA and may modulate pre-mRNA splicing. Binds DNA as a monomer. Interacts with CEBPD and NONO. Interacts with RUNX1 and SPIB. Interacts with GFI1; the interaction represses SPI1 transcriptional activity. Highly expressed in both FV-P and FV-A-induced erythro- leukemia cell lines that have undergone rearrangements of the Spi- 1 gene due to the insertion of SFFV. Belongs to the ETS family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Oncoprotein; Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 11p11.2

Cellular Component: nuclear chromatin

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; NFAT protein binding; RNA binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; macrophage differentiation; granulocyte differentiation; somatic stem cell maintenance; positive regulation of transcription, DNA-dependent; myeloid dendritic cell differentiation; lymphoid progenitor cell differentiation; negative regulation of transcription from RNA polymerase II promoter; anatomical structure regression; regulation of erythrocyte differentiation; regulation of transcription from RNA polymerase II promoter; lymphocyte differentiation; negative regulation of gene expression, epigenetic; negative regulation of MHC class II biosynthetic process; erythrocyte differentiation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent