Typical Testing Data/Standard Curve (for reference only)
Mouse Nucleolin (NCL) ELISA Kit | NCL elisa kit
Mouse Nucleolin (NCL) ELISA Kit
Gene Names
NCL; C23
Reactivity
Mouse
Synonyms
Nucleolin (NCL); Mouse Nucleolin (NCL) ELISA Kit; ELISA Kit for Nucleolin (NCL); NCL elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of nucleolin. No significant cross-reactivity or interference between nucleolin and analogues was observed.
Samples
Mouse tissue homogenates, Cell lysates and other biological fluids
Detection Range
0.156-10ng/mL
Sensitivity
The minimum detectable dose of nucleolin is typically less than 0.059ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level nucleolin were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level nucleolin were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Intended Uses
The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of nucleolin in mouse tissue homogenates, cell lysates and other biological fluids.
Preparation and Storage
For unopened kit, all the reagents should be kept according to the label on each vial. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C while others should be at 4 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for NCL elisa kit
Principle of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to nucleolin. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to nucleolin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain nucleolin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of nucleolin in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
Research Articles on NCL
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Product Notes
The Mouse NCL (Catalog #AAA2000178) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2000178 ELISA Kit recognizes Mouse NCL. It is sometimes possible for the material contained within the vial of "Nucleolin (NCL), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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