Western Blot (WB)
(The recombinant proteins and tissue lysate were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Crystallin alpha A antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. This antibody is not shown cross-activity about Crystallin alpha B.A : Recombinant human Crystallin alpha A proteinB : Recombinant human Crystallin alpha B proteinC : Mouse eye tissue lysate)
Mouse anti-Human A crystallin A Monoclonal Antibody | anti-CRYAA antibody
A crystallin A antibody
For long term storage, aliquot and store at -20°C to -80°C.
Avoid repeated freezing and thawing cycles.
Western Blot (WB)
(The recombinant proteins and tissue lysate were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Crystallin alpha A antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. This antibody is not shown cross-activity about Crystallin alpha B.A : Recombinant human Crystallin alpha A proteinB : Recombinant human Crystallin alpha B proteinC : Mouse eye tissue lysate)
Western Blot (WB)
(The recombinant proteins and tissue lysate were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Crystallin alpha A antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. This antibody is not shown cross-activity about Crystallin alpha B.A : Recombinant human Crystallin alpha A proteinB : Recombinant human Crystallin alpha B proteinC : Mouse eye tissue lysate)
Western Blot (WB)
(The cell lysates (5ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Crystallin alpha A antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: 293T cell lysateLane 2.: Crystallin alpha A Transfected 293T cell lysate)
Western Blot (WB)
(The cell lysates (5ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Crystallin alpha A antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: 293T cell lysateLane 2.: Crystallin alpha A Transfected 293T cell lysate)
Flow Cytommetry (FC)
(Flow cytometry analysis of Crystallin alpha A in Balb/3T3 cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).)
Flow Cytommetry (FC)
(Flow cytometry analysis of Crystallin alpha A in Balb/3T3 cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).)
NCBI and Uniprot Product Information
NCBI Description
Mammalian lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Post-translational modifications decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Defects in this gene cause autosomal dominant congenital cataract (ADCC). [provided by RefSeq, Jan 2014]
Uniprot Description
Contributes to the transparency and refractive index of the lens. Has chaperone-like activity, preventing aggregation of various proteins under a wide range of stress conditions.
Research Articles on CRYAA
Similar Products
alpha A Crystallin Antibody
alpha A Crystallin Antibody
alpha A Crystallin Antibody
Product Notes
The CRYAA cryaa (Catalog #AAA200007) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The A crystallin An antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's A crystallin A can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), FACS. Since applications varies, however, each investigation should be titrated by reagent to obtain optimal results. Researchers should empirically determine the suitability of the CRYAA cryaa for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "A crystallin A, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
