Keratinocyte Growth Factor (rHuKGF) Active Protein | FGF7 active protein

Recombinant Human Keratinocyte Growth Factor

Cat. Num. AAA197014

• Gene Names: FGF7; KGF; HBGF-7
• Purity: Greater than 97% by RP-HPLC, anion-exchange chromatography; reducing and non-reducing SDS-PAGE, silver stain.; Purification: Chromatographic
• Synonyms: Keratinocyte Growth Factor (rHuKGF); Recombinant Human Keratinocyte Growth Factor; Recombinant Human Keratinocyte Growth Factor (rHuKGF); FGF7 active protein

• Host: E Coli
• Specificity: Recombinant Human Keratinocyte Growth Factor (rHuKGF)
• Purity/Purification: Greater than 97% by RP-HPLC, anion-exchange chromatography; reducing and non-reducing SDS-PAGE, silver stain.; Purification: Chromatographic
• Form/Format: Sterile filtered, lyophilized.

• Dimers/Aggregates: <1% by SDSPAGE
• Biological Activity: Fully biologically active compared to reference standards. Specific Activity is 105 Units/mg.
• Endotoxin Level: <0.1 ng/ug
• Protein Content: Determined spectrophotometrically at 280nm (extinction coefficient 0.9) and by RPHPLC analysis against a calibrated standard.
• Solubility: Reconstitute the lyophilized product in sterile water not less than 100 ug/ml.
• Stabilizer: None
• Preservatives: None
• Preparation and Storage: Lyophilized product is stable at room temperature for 3 weeks. Store lyophilized product desiccated at -20 degree C. Upon reconstitution, store at 4 degree C for 2-7 days or aliquot and store at -20 degree C. For long-term storage add either HSA or BSA to 0.1%. Avoid repeated freeze-thaw cycles.

Related Product Information for FGF7 active protein

Recombinant Human KGF produced in E. coli is a single, non-glycosylated, polypeptide chain containing 164 amino acids with a molecular mass of 18,995 daltons. This product has full biological activity.

Product Categories/Family for FGF7 active protein

Recombinant Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 4503705
• NCBI GeneID: 2252
• NCBI Accession #: NP_002000.1
• NCBI GenBank Nucleotide #: NM_002009.3
• UniProt Accession #: P21781; Q6FGV5; Q6RK68; Q96FG5
• Molecular Weight: 22,509 Da
• NCBI Official Full Name: keratinocyte growth factor
• NCBI Official Synonym Full Names: fibroblast growth factor 7
• NCBI Official Symbol: FGF7
• NCBI Official Synonym Symbols: KGF; HBGF-7
• NCBI Protein Information: keratinocyte growth factor; FGF-7; OTTHUMP00000162252; heparin-binding growth factor 7
• UniProt Protein Name: Keratinocyte growth factor
• Protein Family: Fibroblast growth factor
• UniProt Gene Name: FGF7
• UniProt Synonym Gene Names: KGF
• UniProt Entry Name: FGF7_HUMAN

NCBI Description

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein is a potent epithelial cell-specific growth factor, whose mitogenic activity is predominantly exhibited in keratinocytes but not in fibroblasts and endothelial cells. Studies of mouse and rat homologs of this gene implicated roles in morphogenesis of epithelium, reepithelialization of wounds, hair development and early lung organogenesis. [provided by RefSeq]

Uniprot Description

FGF7: Plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. Required for normal branching morphogenesis. Growth factor active on keratinocytes. Possible major paracrine effector of normal epithelial cell proliferation. Belongs to the heparin-binding growth factors family.

Protein type: Cytokine; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 15q21.2

Cellular Component: Golgi apparatus; extracellular region

Molecular Function: heparin binding; protein binding; growth factor activity; chemoattractant activity; fibroblast growth factor receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; positive regulation of keratinocyte migration; epidermis development; hair follicle morphogenesis; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; nerve growth factor receptor signaling pathway; mesenchymal cell proliferation; signal transduction; positive regulation of peptidyl-tyrosine phosphorylation; positive chemotaxis; positive regulation of cell division; positive regulation of cell proliferation; actin cytoskeleton reorganization; response to wounding; insulin receptor signaling pathway; innate immune response; positive regulation of epithelial cell proliferation