Rabbit Caspase-10 (CT) Antibody | anti-CASP10 antibody

Anti-Caspase-10 (CT) Antibody

Cat. Num. AAA194044

• Gene Names: CASP10; MCH4; ALPS2; FLICE2
• Applications: Immunoblot, Western Blot
• Synonyms: Caspase-10 (CT); Anti-Caspase-10 (CT) Antibody; Anti-Caspase-10 (CT); anti-CASP10 antibody

• Host: Rabbit
• Specificity: Anti-Caspase-10 (CT) [Rabbit antibody to peptide corresponding to aa 505-521 at C-terminus of human caspase-10.]; This antibody recognizes full-length human caspase-10 (59 kD). Since the sequences at C-termini of FLICE2 and Mch4 are different, this antibody recognizes only the FLICE2 form of caspase-10.
• Form/Format: 100 ug IgG in PBS, pH 7.4, purified by immunoaffinity chromatography.

• Applicable Applications for anti-CASP10 antibody: Immunoblot
• Application Notes: Immunoblotting: use at 1:500-1:1,000 dilution.; Positive control: Whole cell lysate from HeLa cells.
• Antigen: Peptide corresponding to aa 505- 521 at C-terminus of human FLICE2.
• Stabilizer: None
• Preservatives: 0.02% sodium azide.
• Dilutions Instructions: Dilute in PBS or medium which is identical to that used in the assay system.
• Preparation and Storage: This antibody is stable for at least one (1) year at -20 degree C. Avoid multiple freeze-thaw cycles.

Related Product Information for anti-CASP10 antibody

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain-containing adapter molecules and members of the ICE/CED-3 family of proteases. A novel ICE/CED-3 protease was identified recently, designated FLICE2 and Mch4, and renamed as caspase-10. Caspase-10 has two death effector domains (DEDs) that bind to the DED in the adapter molecule FADD and recruits both TNFR1 and CD95 to form complexes with these receptors. Caspase-10 is, therefore, involved in CD95- and TNFR1-induced apoptosis. Caspase- 10 cleaves and activates caspase-3, -4, -6, -7, -8, and -9, thereby causing proteolytic cleavage of many key proteins, such as PARP. Cleavage of PARP occurs in many different systems during apoptosis and is the hallmark of programmed cell death. Caspase-10 is expressed in many tissues and cell lines.

Product Categories/Family for anti-CASP10 antibody

Apoptosis Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 47078269
• NCBI GeneID: 843
• NCBI Accession #: NP_116756.2
• NCBI GenBank Nucleotide #: NM_032974.4
• UniProt Accession #: Q92851; Q6KF62; Q6KF63; Q8IUP5; Q8WYQ8; Q99845; Q9Y2U6; Q9Y2U7; Q68HC0
• Molecular Weight: 58,951 Da
• NCBI Official Full Name: caspase-10 isoform 2 preproprotein
• NCBI Official Synonym Full Names: caspase 10, apoptosis-related cysteine peptidase
• NCBI Official Symbol: CASP10
• NCBI Official Synonym Symbols: MCH4; ALPS2; FLICE2
• NCBI Protein Information: caspase-10; CASP-10; FADD-like ICE2; OTTHUMP00000163712; OTTHUMP00000163713; OTTHUMP00000163714; OTTHUMP00000206550; OTTHUMP00000206551; apoptotic protease MCH-4; ICE-like apoptotic protease 4; interleukin-1B-converting enzyme 2; caspase 10, apoptosis-related cysteine protease; FAS-associated death domain protein interleukin-1B-converting enzyme 2
• UniProt Protein Name: Caspase-10
• Protein Family: Caspase
• UniProt Gene Name: CASP10
• UniProt Synonym Gene Names: MCH4
• UniProt Entry Name: CASPA_HUMAN

NCBI Description

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 3 and 7, and the protein itself is processed by caspase 8. Mutations in this gene are associated with type IIA autoimmune lymphoproliferative syndrome, non-Hodgkin lymphoma and gastric cancer. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq]

Uniprot Description

CASP10: Involved in the activation cascade of caspases responsible for apoptosis execution. Recruited to both Fas- and TNFR-1 receptors in a FADD dependent manner. May participate in the granzyme B apoptotic pathways. Cleaves and activates caspase- 3, -4, -6, -7, -8, and -9. Hydrolyzes the small- molecule substrates, Tyr-Val-Ala-Asp-|-AMC and Asp-Glu-Val-Asp-|-AMC. Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 23/17 kDa (p23/17) (depending on the splicing events) and a 12 kDa (p12) subunit. Self-associates. Interacts with FADD and CASP8. Found in a Fas signaling complex consisting of FAS, FADD, CASP8 and CASP10. Detectable in most tissues. Lowest expression is seen in brain, kidney, prostate, testis and colon. Belongs to the peptidase C14A family. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.22.63; Protease

Chromosomal Location of Human Ortholog: 2q33-q34

Cellular Component: cytoplasm; plasma membrane; CD95 death-inducing signaling complex; cytosol

Molecular Function: protein binding; ubiquitin protein ligase binding; cysteine-type endopeptidase activity

Biological Process: regulation of apoptosis; positive regulation of I-kappaB kinase/NF-kappaB cascade; apoptosis; innate immune response; proteolysis

Disease: Lymphoma, Non-hodgkin, Familial; Gastric Cancer; Autoimmune Lymphoproliferative Syndrome, Type Iia