Superoxide Dismutase Active Protein | SOD2 active protein

Human Mn Superoxide Dismutase

Cat. Num. AAA191095

• Gene Names: SOD2; IPOB; IPO-B; MNSOD; MVCD6; Mn-SOD
• Purity: Greater than 95% as determined by SDS- PAGE.
• Synonyms: Superoxide Dismutase; Human Mn Superoxide Dismutase; SOD2 active protein

• Host: Escherichia coli
• Purity/Purification: Greater than 95% as determined by SDS- PAGE.
• Form/Format: Sterile-filtered solution in 20mM Tris-HCl, pH 8.0, 20% glycerol. Purified by standard chromatographic techniques.
• Sequence: MGSSHHHHHH SSGLVPRGSH MKHSLPDLPY DYGALEPHIN AQIMQLHHSK HHAAYVNNLN VTEEKYQEAL AKGDVTAQIA LQPALKFNGG GHINHSIFWT NLSPNGGGEP KGELLEAIKR DFGSFDKFKE KLTAASVGVQ GSGWGWLGFN KERGHLQIAA CPNQDPLQGT TGLIPLLGID VWEHAYYLQY KNVRPDYLKA IWNVINWENV TERYMACKK
• Sequence Length: 222

• Biological Activity: Specific activity is >1,200 units/mg in which one unit will inhibit the rate of reduction of cytochrome c by 50% in a coupled system using xanthine and xanthine oxidase at pH 7.8 at 25oC in a 1.5ml reaction volume.
• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks. Store at -20 degree C for longer periods of time. Addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid multiple freeze-thaw cycles.

Related Product Information for SOD2 active protein

Recombinant Human Mn SOD2 produced in E Coli is a single, non-glycosylated polypeptide chain containing 219 amino acids (aa 25-222) with a molecular weight of 24.4kDa. It has a His- tag at the N-terminus.
Glutathione reductase, also known as glutathione-disulfide reductase, is an enzyme that in humans is encoded by the GSR gene. Glutathione reductase catalyzes the reduction of glutathione disulfide to the sulfhydryl form glutathione (GSH), which is a critical molecule in resisting oxidative stress and maintaining the reducing environment of the cell. The importance of the glutathione pathway and enzymes that affect it is gaining an increased level of attention. Although glutathione reductase has been an attractive target for many pharmaceuticals, there have been no successful glutathione reductase related therapeutic compounds created to date.

NCBI and Uniprot Product Information

• NCBI GI #: 67782305
• NCBI GeneID: 6648
• NCBI Accession #: NP_000627.2
• NCBI GenBank Nucleotide #: NM_000636.3
• UniProt Accession #: P04179; P78434; Q16792; Q5TCM1; Q96EE6; Q9P2Z3; B2R7R1; B3KUK2; B4DL20; B4E3K9; E1P5A9
• Molecular Weight: 19,730 Da
• NCBI Official Full Name: superoxide dismutase
• NCBI Official Synonym Full Names: superoxide dismutase 2
• NCBI Official Symbol: SOD2
• NCBI Official Synonym Symbols: IPOB; IPO-B; MNSOD; MVCD6; Mn-SOD
• NCBI Protein Information: superoxide dismutase [Mn], mitochondrial
• UniProt Protein Name: Superoxide dismutase [Mn], mitochondrial
• Protein Family: Superoxide dismutase
• UniProt Gene Name: SOD2

NCBI Description

This gene is a member of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Alternative splicing of this gene results in multiple transcript variants. A related pseudogene has been identified on chromosome 1. [provided by RefSeq, Apr 2016]

Uniprot Description

SOD2: Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems. Genetic variation in SOD2 is associated with susceptibility to microvascular complications of diabetes type 6 (MVCD6). These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new- onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Belongs to the iron/manganese superoxide dismutase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.15.1.1; Mitochondrial; Oxidoreductase

Chromosomal Location of Human Ortholog: 6q25.3

Cellular Component: mitochondrial matrix; mitochondrion

Molecular Function: identical protein binding; manganese ion binding; superoxide dismutase activity

Biological Process: acetylcholine vasodilation involved in regulation of systemic arterial blood pressure; age-dependent response to reactive oxygen species; negative regulation of cell proliferation; negative regulation of neuron apoptosis; oxygen homeostasis; protein homotetramerization; regulation of blood pressure; regulation of transcription from RNA polymerase II promoter; release of cytochrome c from mitochondria; removal of superoxide radicals; response to reactive oxygen species; response to superoxide; superoxide metabolic process

Disease: Microvascular Complications Of Diabetes, Susceptibility To, 6