Mouse Shank2 Monoclonal Antibody | anti-SHANK2 antibody

Anti-Shank2 Monoclonal Antibody

Cat. Num. AAA190606

• Gene Names: SHANK2; SHANK; AUTS17; CORTBP1; CTTNBP1; ProSAP1; SPANK-3
• Reactivity: Human, mouse, rat
• Applications: Immunoblot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
• Synonyms: Shank2; Monoclonal Antibody; Anti-Shank2 Monoclonal Antibody; anti-SHANK2 antibody

• Host: Mouse
• Reactivity: Human, mouse, rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: S23b-6
• Specificity: Shank2; This antibody recognizes human, mouse (weak), and rat Shank2. It does not crossreact with Shank1 or Shank3.
• Form/Format: 100ug in PBS, pH 7.4; 50% glycerol, 0.09% sodium azide. Purified by Protein G affinity chromatography.
• Sequence Positions: 84-309

• Applicable Applications for anti-SHANK2 antibody: Immunoblot; Immunohistochemistry; Immunocytochemistry; Immunofluorescence
• Application Notes: Immunoblotting: use at 1-10ug/ml. A band of ~160kDa is detected. Immunohistochemistry and Immunocytochemistry: use at 0.1-1ug/ml. Immunofluorescence: use at 1-10ug/ml. These are recommended concentrations. User should determine optimal concentrations for their application. Positive control: Adult rat brain lysate.
• Antigen: Fusion protein corresponding to aa 84-309 (SH3/PDZ domains) of rat Shank2 (accession no. Q9QX74). This sequence is 94% homologous with human and 97% homologous with mouse Shank2.
• Dilution Instructions: Dilute in PBS or medium which is identical to that used in the assay system.
• Preparation and Storage: This antibody is stable for at least one (1) year at -20 degree C. Avoid repeated freezing and thawing.

Testing Data

Related Product Information for anti-SHANK2 antibody

Shank proteins are a family of scaffold proteins identified through their interaction with a variety of membrane and cytoplasmic proteins. Shank proteins at postsynaptic sites of excitatory synapses play roles in signal transmission into the postsynaptic neuron. Shank proteins are crucial in receptor tyrosine kinase signaling. Shank2 is expressed in neurons of the developing retina and could play a role in neuronal differentiation of the developing retina. Recent studies suggest that disruption of glutamate receptors at the Shank-postsynaptic platform could contribute to the destruction of post-synaptic density which underlies synaptic dysfunction and loss in Alzheimer's disease.

NCBI and Uniprot Product Information

• NCBI GI #: 254911082
• NCBI GeneID: 22941
• NCBI Accession #: NP_573573.2
• NCBI GenBank Nucleotide #: NM_133266.3
• UniProt Accession #: Q24JS4; Q9UPX8
• Molecular Weight: 26,911 Da
• NCBI Official Full Name: SH3 and multiple ankyrin repeat domains protein 2 isoform 2
• NCBI Official Synonym Full Names: SH3 and multiple ankyrin repeat domains 2
• NCBI Official Symbol: SHANK2
• NCBI Official Synonym Symbols: SHANK; AUTS17; CORTBP1; CTTNBP1; ProSAP1; SPANK-3
• NCBI Protein Information: SH3 and multiple ankyrin repeat domains protein 2; OTTHUMP00000165931; OTTHUMP00000165995; OTTHUMP00000165996; OTTHUMP00000166023; OTTHUMP00000166026; OTTHUMP00000202300; cortactin-binding protein 1; GKAP/SAPAP interacting protein; cortactin SH3 domain-binding protein; proline-rich synapse associated protein 1; proline-rich synapse-associated protein 1
• UniProt Protein Name: SHANK2 protein
• Protein Family: SH3 and multiple ankyrin repeat domains protein
• UniProt Gene Name: SHANK2
• UniProt Entry Name: Q24JS4_HUMAN

NCBI Description

This gene encodes a protein that is a member of the Shank family of synaptic proteins that may function as molecular scaffolds in the postsynaptic density (PSD). Shank proteins contain multiple domains for protein-protein interaction, including ankyrin repeats, an SH3 domain, a PSD-95/Dlg/ZO-1 domain, a sterile alpha motif domain, and a proline-rich region. This particular family member contains a PDZ domain, a consensus sequence for cortactin SH3 domain-binding peptides and a sterile alpha motif. The alternative splicing demonstrated in Shank genes has been suggested as a mechanism for regulating the molecular structure of Shank and the spectrum of Shank-interacting proteins in the PSDs of adult and developing brain. Two alternative splice variants, encoding distinct isoforms, are reported. Additional splice variants exist but their full-length nature has not been determined. [provided by RefSeq]

Uniprot Description

SHANK2: Seems to be an adapter protein in the postsynaptic density (PSD) of excitatory synapses that interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors, and the actin-based cytoskeleton. May play a role in the structural and functional organization of the dendritic spine and synaptic junction. Defects in SHANK2 are a cause of susceptibility to autism type 17 (AUTS17). Autism is a complex multifactorial, pervasive developmental disorder characterized by impairments in reciprocal social interaction and communication, restricted and stereotyped patterns of interests and activities, and the presence of developmental abnormalities by 3 years of age. Most individuals with autism also manifest moderate mental retardation. Belongs to the SHANK family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 11q13.2

Cellular Component: neuron projection; photoreceptor inner segment; brush border membrane; postsynaptic density; dendritic spine; postsynaptic membrane; growth cone; photoreceptor outer segment; cell soma; apical plasma membrane; neurofilament; plasma membrane; ionotropic glutamate receptor complex; cell junction

Molecular Function: ionotropic glutamate receptor binding; protein binding; GKAP/Homer scaffold activity; SH3 domain binding

Biological Process: synaptogenesis; adult behavior; social behavior; learning; memory