Mouse Slo2.2 Sodium-Activated K+ Channel Monoclonal Antibody | anti-Kcnt1 antibody

Anti-Slo2.2 Sodium-Activated K+ Channel Monoclonal Antibody

Cat. Num. AAA190600

• Gene Names: Kcnt1; Slack; rSlo2
• Reactivity: Human, mouse, rat
• Applications: Immunoblot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Protein G purified
• Synonyms: Slo2.2 Sodium-Activated K+ Channel; Monoclonal Antibody; Anti-Slo2.2 Sodium-Activated K+ Channel Monoclonal Antibody; anti-Kcnt1 antibody

• Host: Mouse
• Reactivity: Human, mouse, rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: S3-26
• Specificity: Slo2.2 Sodium-Activated K+ Channel; This antibody recognizes human (weak), mouse, and rat Slo2.2.
• Purity/Purification: Protein G purified
• Concentration: 1.0 mg/ml in PBS, pH 7.4 (varies by lot)

• Applicable Applications for anti-Kcnt1 antibody: Immunoblot; Immunohistochemistry; Immunocytochemistry; Immunofluorescence
• Application Notes: Immunoblotting: use at 1-10ug/ml. A band of ~140kDa is detected. Immunohistochemistry and Immunocytochemistry: use at 0.1-1ug/ml. Immunofluorescence: use at 1-10ug/ml. These are recommended concentrations. User should determine optimal concentrations for their application. Positive control: Rat brain lysate.
• Preservative: 50% Glycerol, 0.09 NaN3
• Antigen: Fusion protein corresponding to aa 1168-1237 of rat Slo2.2 (accession no. NP_068625). This sequence is 94% homologous with human and 98% homologous with mouse Slo2.2.
• Appearance: Clear Liquid
• Dilution Instructions: Dilute in PBS or medium which is identical to that used in the assay system.
• Preparation and Storage: This antibody is stable for at least one (1) year at -20 degree C. Avoid repeated freezing and thawing.

Testing Data

Related Product Information for anti-Kcnt1 antibody

Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient. Slo2.2 is a sodium-activated potassium channel. Slo2.2 channels may contribute to the resting potentials of cells. They also have sensors that couple channel activity to intracellular concentrations of Na+ and Cl-.

NCBI and Uniprot Product Information

• NCBI GI #: 11177892
• NCBI GeneID: 60444
• NCBI Accession #: NP_068625.1
• NCBI GenBank Nucleotide #: NM_021853.1
• UniProt Accession #: Q9Z258; Q5D6C6
• NCBI Official Full Name: potassium channel subfamily T member 1
• NCBI Official Synonym Full Names: potassium channel, subfamily T, member 1
• NCBI Official Symbol: Kcnt1
• NCBI Official Synonym Symbols: Slack; rSlo2
• NCBI Protein Information: potassium channel subfamily T member 1; potassium channel subunit (Slack); sequence like a calcium-activated potassium channel subunit
• UniProt Protein Name: Potassium channel subfamily T member 1
• Protein Family: Potassium channel subfamily
• UniProt Gene Name: Kcnt1
• UniProt Synonym Gene Names: Slack
• UniProt Entry Name: KCNT1_RAT

NCBI Description

Na(+)-activated potassium channel; may be involved in regulating the firing properties of neurons [RGD]

Uniprot Description

KCNT1: Outwardly rectifying potassium channel subunit that may co-assemble with other Slo-type channel subunits. Activated by high intracellular sodium or chloride levels. Activated upon stimulation of G-protein coupled receptors, such as CHRM1 and GRIA1. May be regulated by calcium in the absence of sodium ions (in vitro). Belongs to the potassium channel family. Calcium- activated (TC 1.A.1.3) subfamily. KCa4.1/KCNT1 sub-subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Channel, potassium; Membrane protein, multi-pass

Cellular Component: voltage-gated potassium channel complex; membrane; integral to membrane

Molecular Function: voltage-gated potassium channel activity; calcium-activated potassium channel activity; potassium channel activity

Biological Process: potassium ion transport