Mouse FKBP52 Monoclonal Antibody
FKBP52 Monoclonal Antibody
Applications
Immunoblot, Western Blot, Immunohistochemistry, Immunoprecipitation
Synonyms
FKBP52; Monoclonal Antibody; FKBP52 Monoclonal Antibody; anti-FKBP52 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Specificity
FKBP52 Monoclonal Antibody [Mouse monoclonal antibody recognizes FKBP52 of multiple species.]
This antibody recognizes human, mouse, rat, dog, and hamster FKBP52.
This antibody recognizes human, mouse, rat, dog, and hamster FKBP52.
Form/Format
100ug in PBS, pH 7.4, purified by Protein G affinity chromatography.
Applicable Applications for anti-FKBP52 antibody
Immunoblot, Immunohistochemistry, Immunoprecipitation
Application Notes
Immunoblotting: use at 0.5-1ug/ml. A band of ~52 kDa is detected.
Immunohistochemistry: use at 4-5ug/ml
Immunoprecipitation: 5ug on 10-20ul Protein A-Sepharose + 100ul sample. These are recommended concentrations. User should determine optimal concentrations for their application.
Positive control: HeLa cell lysate, rat kidney and rat/mouse testes tissue extracts.
Immunohistochemistry: use at 4-5ug/ml
Immunoprecipitation: 5ug on 10-20ul Protein A-Sepharose + 100ul sample. These are recommended concentrations. User should determine optimal concentrations for their application.
Positive control: HeLa cell lysate, rat kidney and rat/mouse testes tissue extracts.
Antigen
Recombinant full-length FKBP52 with an N-terminal polyhistidine tag.
Preservatives
0.09% sodium azide
Other Additives
50% glycerol
Dilutions Instructions
Dilute in PBS or medium which is identical to that used in the assay system.
Preparation and Storage
This antibody is stable for at least one (1) year at -20 degree C. Avoid repeated freezing and thawing.
Related Product Information for anti-FKBP52 antibody
FK506-binding proteins, FKBP51 and FKBP52, are high molecular weight immunophilins that are part of the mature glucocorticoid receptor heterocomplex. The N-terminal domains of these proteins bind FK506 and have peptidyl-prolyl isomerase activity that converts prolyl peptide bonds within target proteins from cis- to trans- proline. The C-terminal domains contain the tetratricopeptide repeats that are involved in protein-protein interactions with Hsp90. Although FKBP52 and FKBP51 share ~75% sequence similarity, they affect hormone binding by glucocorticoid receptors differently and have different Hsp90-binding characteristics. In addition, FKBP51 is linked to the progesterone receptor while FKBP52 is linked to progesterone, androgen, and glucocorticoid receptors.
Product Categories/Family for anti-FKBP52 antibody
References
Nair, SC et al. 1997 Mol Cell Biol 17: 594- 603
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Product Notes
The FKBP52 (Catalog #AAA190476) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's FKBP52 can be used in a range of immunoassay formats including, but not limited to, Immunoblot, Immunohistochemistry, Immunoprecipitation. Immunoblotting: use at 0.5-1ug/ml. A band of ~52 kDa is detected. Immunohistochemistry: use at 4-5ug/ml Immunoprecipitation: 5ug on 10-20ul Protein A-Sepharose + 100ul sample. These are recommended concentrations. User should determine optimal concentrations for their application. Positive control: HeLa cell lysate, rat kidney and rat/mouse testes tissue extracts. Researchers should empirically determine the suitability of the FKBP52 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FKBP52, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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