Hamster Glutamate Oxaloacetate Transaminase 1 (GOT1) ELISA Kit | GOT1 elisa kit

Hamster Glutamate Oxaloacetate Transaminase 1 (GOT1) ELISA Kit

Cat. Num. AAA19017

• Gene Names: ASP5; AAT3; ASP5; ASPARTATE AMINOTRANSFERASE; aspartate aminotransferase 5; ASPARTATE AMINOTRANSFERASE DEFICIENT 3; ATAAT1; F11C18.15
• Reactivity: Hamster
• Synonyms: Glutamate Oxaloacetate Transaminase 1 (GOT1); Hamster Glutamate Oxaloacetate Transaminase 1 (GOT1) ELISA Kit; Aspartate aminotransferase; cytoplasmic (GOT1); GIG18; aspartate aminotransferase 1; growth-inhibiting protein 18; glutamic-oxaloacetic transaminase 1; soluble (aspartate aminotransferase 1); cAspAT; Cysteine aminotransferase; cytoplasmic; cCAT; GOT1 elisa kit

• Reactivity: Hamster
• Specificity: No significant cross-reactivity or interference between Hamster AST and analogues was observed.

• Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
• Assay Type: Sandwich
• Detection Range: 5.0U/L-160U/L.
• Sensitivity: 1.0U/L.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated AST concentrations in Hamster serum, plasma and other body fluids. Using Purified Hamster AST antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add AST and AST antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of AST in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for GOT1 elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated AST concentrations in Hamster serum, plasma and other body fluids. Using Purified Hamster AST antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add AST and AST antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of AST in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 334187077
• NCBI GeneID: 829330
• NCBI Accession #: NP_001190885.1
• NCBI GenBank Nucleotide #: NM_001203956.1
• UniProt Accession #: P46248; O49392; Q8LGE6
• Molecular Weight: 49,831 Da
• NCBI Official Full Name: aspartate aminotransferase
• NCBI Official Symbol: ASP5
• NCBI Official Synonym Symbols: AAT3; ASP5; ASPARTATE AMINOTRANSFERASE; aspartate aminotransferase 5; ASPARTATE AMINOTRANSFERASE DEFICIENT 3; ATAAT1; F11C18.15
• NCBI Protein Information: aspartate aminotransferase
• UniProt Protein Name: Aspartate aminotransferase, chloroplastic
• Protein Family: Protein transport protein
• UniProt Gene Name: ASP5
• UniProt Synonym Gene Names: AAT1
• UniProt Entry Name: AAT5_ARATH

NCBI Description

encodes a plastid-localized aspartate aminotransferase

Uniprot Description

Function: Important for the metabolism of amino acids and Krebs-cycle related organic acids. In plants, it is involved in nitrogen metabolism and in aspects of carbon and energy metabolism.

Catalytic activity: L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate.

Cofactor: Pyridoxal phosphate.

Subunit structure: Homodimer

By similarity.

Subcellular location: Plastid › chloroplast.

Miscellaneous: In eukaryotes there are cytoplasmic, mitochondrial and chloroplastic isozymes.

Sequence similarities: Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.