Mouse CD16 Monoclonal Antibody | anti-CD16 antibody

Anti-Hu CD16 Pacific Orange

• Reactivity: Non-human primates, Human
• Applications: Flow Cytometry, Functional Assay
• Purity: The purified antibody is conjugated with Pacific Orange under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Synonyms: CD16; Monoclonal Antibody; Anti-Hu CD16 Pacific Orange; FcgammaRIII; IGFR3; FCRIII; anti-CD16 antibody

• Host: Mouse
• Reactivity: Non-human primates, Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1 kappa
• Clone Number: 3G8
• Specificity: The mouse monoclonal antibody 3G8 recognizes an extracellular epitope of CD16, a low affinity receptor for aggregated IgG (FcgammaRIII antigen). CD16 exists in two different isoforms: CD16a (FcgammaRIIIA; 50-65kDa; expressed on NK-cells, monocytes and macrophages) and CD16b (FcgammaRIIIB; 48kDa; mainly expressed on neutrophils).
• Purity/Purification: The purified antibody is conjugated with Pacific Orange under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Form/Format: Pacific Orange. Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide

• Applicable Applications for anti-CD16 antibody: Flow Cytometry (FC/FACS) (QC tested)
• Application Notes: FC/FACS: The reagent is designed for analysis of human blood cells using 4ul reagent/100ul of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
• Immunogen: Human neutrophils
• Preparation and Storage: Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.

Flow Cytometry (FC/FACS)

(Flow cytometry analysis (surface staining) of CD16 in human peripheral blood with anti-CD16 (3G8) Pacific Orange™.)

Related Product Information for anti-CD16 antibody

CD16 (FcgammaRIII) is a 50-65kDa glycoprotein serving as a low affinity IgG receptor. Human FcgammaRIII is expressed in two forms FcgammaRIII-A and -B. FcgammaRIII-A is a transmembrane protein of monocytes, macrophages, NK cells and a subset of T cells. It is associated with FcepsilonRI-gamma subunit and is responsible for antibody-dependent NK cell cytotoxicity. Mast cell FcgammaRIII-A is associated, moreover, with FcepsilonRI-beta subunit. Besides IgG, FcgammaRIII-A can be triggered also by oligomeric IgE. FcgammaRIII-B is a GPI-linked monomeric receptor expressed on neutrophils and is involved in their activation and induction of a proadhesive phenotype.

References

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