Mouse anti-Human Ig Kappa Light Chain Monoclonal Antibody

Anti-Hu Ig Kappa Light Chain Pacific Blue

• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: The purified antibody is conjugated with Pacific Blue under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Synonyms: Ig Kappa Light Chain; Monoclonal Antibody; Anti-Hu Ig Kappa Light Chain Pacific Blue; Immunoglobulin kappa; Igk; anti-Ig Kappa Light Chain antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone Number: A8B5
• Specificity: The mouse monoclonal antibody A8B5 reacts with kappa light chains (22.5kDa) of immunoglobulins.
• Purity/Purification: The purified antibody is conjugated with Pacific Blue under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Form/Format: Pacific Blue. Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide

• Applicable Applications for anti-Ig Kappa Light Chain antibody: Flow Cytometry (FC/FACS) (QC tested)
• Application Notes: FC/FACS: The reagent is designed for analysis of human blood cells using 4ul reagent/100ul of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
• Negative Species: Rabbit, Hamster, Sheep, Guinea pig, Goat
• Preparation and Storage: Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.

Flow Cytometry (FC/FACS)

(Flow cytometry analysis (surface staining) of immunoglobulin kappa light chains in human peripheral blood with anti-kappa light chains (A8B5) Pacific Blue™.)

Related Product Information for anti-Ig Kappa Light Chain antibody

Immunoglobulin classes share the same basic four polypeptide chain structure of two heavy chains (five heavy chains types) and two light chains (kappa, lambda; both having a molecular weight of 22.5kDa). Kappa and lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of kappa to lambda is 70:30.

References

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*Franklin EC: Structure and Functional Application (FUNC)tion of immunoglobulins. Acta Endocrinol Suppl (Copenh). 1975;194:77-95., URL: https://www.ncbi.nlm.nih.gov/pubmed/47690ordinalp