Mouse anti-Mouse, Human CD3 zeta Monoclonal Antibody | anti-CD3zeta antibody

Anti-Hu CD3 zeta (pY72) Alexa Fluor647

• Gene Names: CD247; T3Z; CD3H; CD3Q; CD3Z; TCRZ; CD3-ZETA
• Reactivity: Mouse, Human
• Applications: Flow Cytometry, Functional Assay, Immunocytochemistry
• Purity: The purified antibody is conjugated with Alexa Fluor647 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Synonyms: CD3 zeta; Monoclonal Antibody; Anti-Hu CD3 zeta (pY72) Alexa Fluor647; CD3 zeta chain; CD247; T3Z; TCRz; IMD25; CD247 molecule; anti-CD3zeta antibody

• Host: Mouse
• Reactivity: Mouse, Human
• Clonality: Monoclonal
• Isotype: Mouse IgG2b
• Clone Number: EM-26
• Specificity: The mouse monoclonal antibody EM-26 recognizes phosphorylated intracellular tyrosine 72 of CD3 zeta chain (CD247), which is a component of TCR/CD3 complex expressed on T cells.
• Purity/Purification: The purified antibody is conjugated with Alexa Fluor647 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Form/Format: Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide
• Concentration: 0.1mg/ml (varies by lot)

• Applicable Applications for anti-CD3zeta antibody: Flow Cytometry (FC/FACS) (QC tested), Immunocytochemistry (ICC)
• Application Notes: FC/FACS: Intracellular staining; recommended dilution: 2-6ug/ml; positive control: Jurkat cells treated with pervanadate, T-cells from lymph nodes of OT-1 mouse treated with pervanadate.
• Conjugation: Alexa Fluor647
• Immunogen: A phospho specific peptide corresponding to the amino acids surrounding tyrosine 72 of mouse CD3 zeta linked to KLH
• Preparation and Storage: Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.

Flow Cytometry (FC/FACS)

(Separation of CD3 positive T cells stained using anti-human CD3 zeta (pY72) Alexa Fluor 647 antibody (concentration in sample 3 ug/ml, red-filled) from T cells stained using mouse IgG1 isotype control (MOPC-21) Alexa Fluor 647 antibody (concentration in sample 3 ug/ml, black-dashed) in flow cytometry analysis (intracellular staining) of human PHA activated peripheral blood mononuclear cells.)

Related Product Information for anti-CD3zeta antibody

CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta (CD247). These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation.

References

*Dopfer EP, Schöpf B, Louis-Dit-Sully C, Dengler E, Höhne K, Klescová A, Prouza M, Suchanek M, Reth M, Schamel WW: Analysis of novel phospho-ITAM specific antibodies in a S2 reconstitution system for TCR-CD3 signalling. Immunol Lett. 2010 May 4;130(1-2):43-50, URL: http://www.ncbi.nlm.nih.gov/pubmed/20005895,*Borroto A, Arellano I, Dopfer EP, Prouza M, Suchanek M, Fuentes M, Orfao A, Schamel WW, Alarcón B: Nck recruitment to the TCR required for ZAP70 activation during thymic development. J Immunol. 2013 Feb 1;190(3):1103-12., URL: http://www.ncbi.nlm.nih.gov/pubmed/term=borroto+a

NCBI and Uniprot Product Information

• NCBI GI #: 4557431
• NCBI GeneID: 919
• NCBI Accession #: NP_000725.1
• NCBI GenBank Nucleotide #: NM_000734.3
• UniProt Accession #: P20963; Q5VX13; Q8TAX4; B1AK49; A46461
• Molecular Weight: 18,696 Da
• NCBI Official Full Name: T-cell surface glycoprotein CD3 zeta chain isoform 2
• NCBI Official Synonym Full Names: CD247 molecule
• NCBI Official Symbol: CD247
• NCBI Official Synonym Symbols: T3Z; CD3H; CD3Q; CD3Z; TCRZ; CD3-ZETA
• NCBI Protein Information: T-cell surface glycoprotein CD3 zeta chain; CD3zeta chain; TCR zeta chain; CD247 antigen, zeta subunit; T-cell receptor T3 zeta chain; CD3Z antigen, zeta polypeptide (TiT3 complex); T-cell antigen receptor complex, zeta subunit of CD3
• UniProt Protein Name: T-cell surface glycoprotein CD3 zeta chain
• UniProt Gene Name: CD247
• UniProt Synonym Gene Names: CD3Z; T3Z; TCRZ
• UniProt Entry Name: CD3Z_HUMAN

NCBI Description

The protein encoded by this gene is T-cell receptor zeta, which together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and -epsilon, forms the T-cell receptor-CD3 complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

CD3Z: a T cell surface glycoprotein that is a component of the T cell antigen receptor. Plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the CD3-epsilon results in impaired immune response. Two alternatively spliced isoforms have been described.

Protein type: Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q24.2

Cellular Component: T cell receptor complex; cytoplasm; plasma membrane; integral to membrane; alpha-beta T cell receptor complex

Molecular Function: identical protein binding; protein binding; protein homodimerization activity; transmembrane receptor activity

Biological Process: regulation of immune response; viral reproduction; T cell costimulation; innate immune response; T cell receptor signaling pathway; regulation of defense response to virus by virus

Disease: Immunodeficiency 25