anti-Mouse, Human CD3 zeta Monoclonal Antibody | anti-CD3zeta antibody

Anti-CD3 zeta PE-Cy7

• Gene Names: CD247; T3Z; CD3H; CD3Q; CD3Z; TCRZ; CD3-ZETA
• Reactivity: Mouse, Human
• Applications: Flow Cytometry, Functional Assay
• Purity: The purified antibody is conjugated with tandem dye PE-Cy7 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Synonyms: CD3 zeta; Monoclonal Antibody; Anti-CD3 zeta PE-Cy7; CD3 zeta chain; CD247; T3Z; TCRz; IMD25; CD247 molecule; anti-CD3zeta antibody

• Host: Armenian Hamster
• Reactivity: Mouse, Human
• Clonality: Monoclonal
• Isotype: Armenian Hamster IgG2 kappa
• Clone Number: H146-968
• Specificity: The Armenian hamster antibody H146-968 reacts with an intracellular epitope (amino acids 151-164) of CD3 zeta chain (CD247), which is a component of TCR/CD3 complex expressed on T cells.
• Purity/Purification: The purified antibody is conjugated with tandem dye PE-Cy7 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Form/Format: Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide
• Concentration: 0.1mg/ml (varies by lot)

• Applicable Applications for anti-CD3zeta antibody: Flow Cytometry (FC/FACS) (QC tested)
• Application Notes: FC/FACS: Recommended dilution: 1-2ug/ml. Intracellular staining.
• Conjugation: PE-Cy7
• Immunogen: Synthetic peptide corresponding to amino acids 151-164 of mouse CD3 zeta.
• Preparation and Storage: Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.

Flow Cytometry (FC/FACS)

(Flow cytometry intracellular staining pattern of human peripheral whole blood stained using anti-human CD3 zeta (H146-968) PE-Cy™7 antibody (concentration in sample 12 ug/ml).)

Flow Cytometry (FC/FACS)

(Flow cytometry multicolor surface staining pattern of human lymphocytes using anti-human CD3 (UCHT1) APC antibody (10 ul reagent / 100 ul of peripheral whole blood) and intracellular staining of human lymphocytes using anti-human CD3 zeta (H146-968) PE-Cy™7 antibody (concentration in sample 12 ug/ml).)

Flow Cytometry (FC/FACS)

(Separation of human CD3 zeta positive CD3 positive T cells (red-filled) from CD3 zeta negative CD3 negative lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood stained using anti-human CD3 zeta (H146-968) PE-Cy™7 antibody (concentration in sample 12 ug/ml).)

Related Product Information for anti-CD3zeta antibody

CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta (CD247). These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation.

References

*Scumpia PO, Delano MJ, Kelly-Scumpia KM, Weinstein JS, Wynn JL, Winfield RD, Xia C, Chung CS, Ayala A, Atkinson MA, Reeves WH, Clare-Salzler MJ, Moldawer LL: Treatment with GITR agonistic antibody corrects adaptive immune dysFunctional Application (FUNC)tion in sepsis. Blood. 2007 Nov 15;110(10):3673-81., URL: http://www.ncbi.nlm.nih.gov/pubmed/17690255itool=,*Doyon L, Hanna Z, Jolicoeur P, Sékaly RP: Early expression of human CD4 delays thymic differentiation in transgenic mice. Res Immunol. 1994 Jan;145(1):5-16., URL: http://www.ncbi.nlm.nih.gov/pubmed/7912001itool=E,*Kikuchi-Maki A, Catina TL, Campbell KS: Cutting edge: KIR2DL4 transduces signals into human NK cells through association with the Fc receptor gamma protein. J Immunol. 2005 Apr 1;174(7):3859-63., URL: http://www.ncbi.nlm.nih.gov/pubmed/15778339itool=,*Iida T, Ohno H, Nakaseko C, Sakuma M, Takeda-Ezaki M, Arase H, Kominami E, Fujisawa T, Saito T: Regulation of cell surface expression of CTLA-4 by secretion of CTLA-4-containing lysosomes upon activation of CD4+ T cells. J Immunol. 2000 Nov 1;165(9):5062-8., URL: http://www.ncbi.nlm.nih.gov/pubmed/11046036itool=,*Kosugi A, Saitoh S, Noda S, Yasuda K, Hayashi F, Ogata M, Hamaoka T: Translocation of tyrosine-phosphorylated TCRzeta chain to glycolipid-enriched membrane domains upon T cell activation. Int Immunol. 1999 Sep;11(9):1395-401., URL: http://www.ncbi.nlm.nih.gov/pubmed/10464160itool=,*Bäckström BT, Hausmann BT, Palmer E: Signaling efficiency of the T cell receptor controlled by a single amino acid in the beta chain constant region. J Exp Med. 1997 Dec 1;186(11):1933-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/9382891itool=,*Kishimoto H, Kubo RT, Yorifuji H, Nakayama T, Asano Y, Tada T: Physical dissociation of the TCR-CD3 complex accompanies receptor ligation. J Exp Med. 1995 Dec 1;182(6):1997-2006., URL: https://www.ncbi.nlm.nih.gov/pubmed/7500045ordina,*Rozdzial MM, Kubo RT, Turner SL, Finkel TH: Developmental regulation of the TCR zeta-chain. Differential expression and tyrosine phosphorylation of the TCR zeta-chain in resting immature and mature T lymphocytes. J Immunol. 1994 Aug 15;153(4):1563-80., URL: https://www.ncbi.nlm.nih.gov/pubmed/7519206ordina
*Alarcón B, Swamy M, van Santen HM, Schamel WW: T-cell antigen-receptor stoichiometry: pre-clustering for sensitivity. EMBO Rep. 2006 May;7(5):490-5., URL: http://www.ncbi.nlm.nih.gov/sites/entrezDb=pubmed,*Kuhns MS, Davis MM, Garcia KC: Deconstructing the form and Functional Application (FUNC)tion of the TCR/CD3 complex. Immunity. 2006 Feb;24(2):133-9., URL: http://www.ncbi.nlm.nih.gov/sites/entrezDb=pubmed,*Huang Y, Wange RL: T cell receptor signaling: beyond complex complexes. J Biol Chem. 2004 Jul 9;279(28):28827-30., URL: http://www.ncbi.nlm.nih.gov/sites/entrezDb=pubmed

NCBI and Uniprot Product Information

• NCBI GI #: 4557431
• NCBI GeneID: 919
• NCBI Accession #: NP_000725.1
• NCBI GenBank Nucleotide #: NM_000734.3
• UniProt Accession #: P20963; Q5VX13; Q8TAX4; B1AK49; A46461
• Molecular Weight: 18,696 Da
• NCBI Official Full Name: T-cell surface glycoprotein CD3 zeta chain isoform 2
• NCBI Official Synonym Full Names: CD247 molecule
• NCBI Official Symbol: CD247
• NCBI Official Synonym Symbols: T3Z; CD3H; CD3Q; CD3Z; TCRZ; CD3-ZETA
• NCBI Protein Information: T-cell surface glycoprotein CD3 zeta chain; CD3zeta chain; TCR zeta chain; CD247 antigen, zeta subunit; T-cell receptor T3 zeta chain; CD3Z antigen, zeta polypeptide (TiT3 complex); T-cell antigen receptor complex, zeta subunit of CD3
• UniProt Protein Name: T-cell surface glycoprotein CD3 zeta chain
• UniProt Gene Name: CD247
• UniProt Synonym Gene Names: CD3Z; T3Z; TCRZ
• UniProt Entry Name: CD3Z_HUMAN

NCBI Description

The protein encoded by this gene is T-cell receptor zeta, which together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and -epsilon, forms the T-cell receptor-CD3 complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

CD3Z: a T cell surface glycoprotein that is a component of the T cell antigen receptor. Plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the CD3-epsilon results in impaired immune response. Two alternatively spliced isoforms have been described.

Protein type: Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q24.2

Cellular Component: T cell receptor complex; cytoplasm; plasma membrane; integral to membrane; alpha-beta T cell receptor complex

Molecular Function: identical protein binding; protein binding; protein homodimerization activity; transmembrane receptor activity

Biological Process: regulation of immune response; viral reproduction; T cell costimulation; innate immune response; T cell receptor signaling pathway; regulation of defense response to virus by virus

Disease: Immunodeficiency 25