Mouse anti-Human CD107b Monoclonal Antibody | anti-CD107b antibody

Anti-Hu CD107b Alexa Fluor488

• Gene Names: LAMP2; LAMPB; CD107b; LAMP-2; LGP110
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Western Blot, Immunocytochemistry
• Purity: The purified antibody is conjugated with Alexa Fluor488 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Synonyms: CD107b; Monoclonal Antibody; Anti-Hu CD107b Alexa Fluor488; LAMP-2; LAMPB; lysosomal associated membrane protein 2; anti-CD107b antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1 kappa
• Clone Number: H4B4
• Specificity: The mouse monoclonal antibody H4B4 recognizes an extracellular/luminal epitope of CD107b/LAMP-2, an extensively glycosylated 100-120kDa widely expressed lysosome-associated protein.
• Purity/Purification: The purified antibody is conjugated with Alexa Fluor488 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Form/Format: Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide

• Applicable Applications for anti-CD107b antibody: Flow Cytometry (FC/FACS) (QC tested), Western Blot (WB), Immunocytochemistry (ICC)
• Application Notes: FC/FACS: The reagent is designed for analysis of human blood cells using 4ul reagent/100ul of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. Intracellular and extracellular staining.
• Conjugation: Alexa Fluor488
• Negative Species: Mouse, Rat
• Immunogen: Human PBMC
• Preparation and Storage: Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.

Flow Cytometry (FC/FACS)

(Flow cytometry analysis (surface staining) of human peripheral blood with anti-CD107b (H4B4) Alexa Fluor 488.)

Related Product Information for anti-CD107b antibody

CD107b (lysosome-associated membrane protein-2, LAMP-2), together with CD107a/LAMP-1, is a major constituent of lysosomal membrane. The LAMP proteins are involved in lysosome biogenesis and are required for fusion of lysosomes with phagosomes, especially CD107b is important regulator in successful phagosomal maturation. CD107b deficiency causes an accumulation of autophagosomes in many tissues leading to cardiomyopathy and myopathy (Danons disease). Immature CD107b is an approximately 45kDa protein, but after extensive glycosylation the mature glycoprotein has about 100-120kDa.

References

*Apte SH, Baz A, Groves P, Kelso A, Kienzle N: Interferon-gamma and interleukin-4 reciprocally regulate CD8 expression in CD8+ T cells. Proc Natl Acad Sci U S A. 2008 Nov 11;105(45):17475-80., URL: https://www.ncbi.nlm.nih.gov/pubmed/18988742,*Palmer DR, Fernandez S, Bisbing J, Peachman KK, Rao M, Barvir D, Gunther V, Burgess T, Kohno Y, Padmanabhan R, Sun W: Restricted replication and lysosomal trafficking of yellow fever 17D vaccine virus in human dendritic cells. J Gen Virol. 2007 Jan;88(Pt 1):148-56., URL: https://www.ncbi.nlm.nih.gov/pubmed/17170447,*Denzer K, van Eijk M, Kleijmeer MJ, Jakobson E, de Groot C, Geuze HJ: Follicular dendritic cells carry MHC class II-expressing microvesicles at their surface. J Immunol. 2000 Aug 1;165(3):1259-65., URL: https://www.ncbi.nlm.nih.gov/pubmed/10903724,*Thedrez A, Harly C, Morice A, Salot S, Bonneville M, Scotet E: IL-21-mediated potentiation of antitumor cytolytic and proinflammatory responses of human V gamma 9V delta 2 T cells for adoptive immunotherapy. J Immunol. 2009 Mar 15;182(6):3423-31., URL: https://www.ncbi.nlm.nih.gov/pubmed/19265120,*Kannanganat S, Ibegbu C, Chennareddi L, Robinson HL, Amara RR: Multiple-cytokine-producing antiviral CD4 T cells are Functional Application (FUNC)tionally superior to single-cytokine-producing cells. J Virol. 2007 Aug;81(16):8468-76., URL: https://www.ncbi.nlm.nih.gov/pubmed/17553885,*Guia S, Cognet C, de Beaucoudrey L, Tessmer MS, Jouanguy E, Berger C, Filipe-Santos O, Feinberg J, Camcioglu Y, Levy J, Al Jumaah S, Al-Hajjar S, Stephan JL, Fieschi C, Abel L, Brossay L, Casanova JL, Vivier E: A role for interleukin-12/23 in the maturation of human natural killer and CD56+ T cells in vivo. Blood. 2008 May 15;111(10):5008-16., URL: https://www.ncbi.nlm.nih.gov/pubmed/18319400,*Meade JL, Wilson EB, Holmes TD, de Wynter EA, Brett P, Straszynski L, Ballard PA, Trapani JA, McDermott MF, Cook GP: Proteolytic activation of the cytotoxic phenotype during human NK cell development. J Immunol. 2009 Jul 15;183(2):803-13., URL: http://www.ncbi.nlm.nih.gov/pubmed/19570824,*Borgne-Sanchez A, Dupont S, Langonné A, Baux L, Lecoeur H, Chauvier D, Lassalle M, Déas O, Briere JJ, Brabant M, Roux P, Péchoux C, Briand JP, Hoebeke J, Deniaud A, Brenner C, Rustin P, Edelman L, Rebouillat D, Jacotot E: Targeted Vpr-derived peptides reach mitochondria to induce apoptosis of alphaVbeta3-expressing endothelial cells. Cell Death Differ. 2007 Mar;14(3):422-35., URL: http://www.ncbi.nlm.nih.gov/pubmed/16888644

NCBI and Uniprot Product Information

• NCBI GI #: 1708854
• NCBI GeneID: 3920
• UniProt Accession #: P13473; Q16641; Q6Q3G8; Q96J30; Q99534; Q9UD93; A8K4X5; D3DTF0
• Molecular Weight: 410
• NCBI Official Full Name: Lysosome-associated membrane glycoprotein 2
• NCBI Official Synonym Full Names: lysosomal-associated membrane protein 2
• NCBI Official Symbol: LAMP2
• NCBI Official Synonym Symbols: LAMPB; CD107b; LAMP-2; LGP110
• NCBI Protein Information: lysosome-associated membrane glycoprotein 2; CD107 antigen-like family member B
• UniProt Protein Name: Lysosome-associated membrane glycoprotein 2
• UniProt Gene Name: LAMP2
• UniProt Synonym Gene Names: LAMP-2; Lysosome-associated membrane protein 2
• UniProt Entry Name: LAMP2_HUMAN

NCBI Description

The protein encoded by this gene is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. It may play a role in tumor cell metastasis. It may also function in the protection, maintenance, and adhesion of the lysosome. Alternative splicing of this gene results in multiple transcript variants encoding distinct proteins. [provided by RefSeq, Jul 2008]

Uniprot Description

LAMP2: Implicated in tumor cell metastasis. May function in protection of the lysosomal membrane from autodigestion, maintenance of the acidic environment of the lysosome, adhesion when expressed on the cell surface (plasma membrane), and inter- and intracellular signal transduction. Protects cells from the toxic effects of methylating mutagens. Defects in LAMP2 are the cause of Danon disease (DAND); also known as glycogen storage disease type 2B (GSD2B). DAND is a lysosomal glycogen storage disease characterized by the clinical triad of cardiomyopathy, vacuolar myopathy and mental retardation. It is often associated with an accumulation of glycogen in muscle and lysosomes. Belongs to the LAMP family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: Xq24

Cellular Component: phagocytic vesicle membrane; membrane; lysosome; lysosomal membrane; late endosome membrane; late endosome; plasma membrane; integral to membrane; platelet dense granule membrane

Molecular Function: protein domain specific binding; enzyme binding

Biological Process: platelet activation; platelet degranulation; protein stabilization; blood coagulation

Disease: Danon Disease