Rabbit ADFP Polyclonal Antibody | anti-PLIN2 antibody

Anti-ADFP Antibody

Cat. Num. AAA178839

• Gene Names: PLIN2; ADFP; ADRP
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen affinity purified.
• Synonyms: ADFP; Polyclonal Antibody; Anti-ADFP Antibody; Adipophilin; ADRP; Perilipin-2; PLIN2; Q99541; perilipin 2; anti-PLIN2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 437

• Applicable Applications for anti-PLIN2 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Application Notes: Western Blot: 0.1-0.5ug/ml; Tested Species: Human;; Immunohistochemistry(IHC) Paraffin: 0.5-1ug/ml; Tested Species: Human, Mouse, Rat; Antigen Retrieval: By Heat; ; Tested Species: In-house tested species with positive results.; By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.; ; Other applications have not been tested.; Optimal dilutions should be determined by end users.
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Immunogen: E. coli-derived human ADFP recombinant protein (Position: K226-Q418). Human ADFP shares 88.4% amino acid (aa) sequence identity with mouse ADFP.
• Preparation and Storage: Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Western blot analysis of ADFP expression in MCF-7 whole cell lysates (lane 1). ADFP at 48KD was detected using rabbit anti- ADFP Antigen Affinity purified polyclonal antibody at 0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method. )

Immunohistochemistry (IHC)

(ADFP was detected in paraffin-embedded sections of mouse testis tissues using rabbit anti- ADFP Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method. )

Immunohistochemistry (IHC)

(ADFP was detected in paraffin-embedded sections of rat testis tissues using rabbit anti- ADFP Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method. )

Immunohistochemistry (IHC)

(ADFP was detected in paraffin-embedded sections of human mammary cancer tissues using rabbit anti- ADFP Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method. )

Related Product Information for anti-PLIN2 antibody

Rabbit IgG polyclonal antibody for Perilipin-2(PLIN2) detection.
Background: Adipose differentiation-related protein, also known as perilipin 2 (PLIN2), ADRP or adipophilin, is a protein which in humans is encoded by the ADFP gene. The protein encoded by this gene belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This protein is associated with the lipid globule surface membrane material, and maybe involved in development and maintenance of adipose tissue. However, it is not restricted to adipocytes as previously thought, but is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells, and tissues, such as lactating mammary gland, adrenal cortex, Sertoli and Leydig cells, and hepatocytes in alcoholic liver cirrhosis, suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases. Alternatively spliced transcript variants have been found for this gene.

References

1. Heid, H. W., Schnolzer, M., Keenan, T. W. Adipocyte differentiation-related protein is secreted into milk as a constituent of milk lipid globule membrane. Biochem. J. 320: 1025-1030, 1996.
2. Shaw, C. S., Sherlock, M., Stewart, P. M., Wagenmakers, A. J. M.Adipophilin distribution and colocalisation with lipid droplets in skeletal muscle. Histochem. Cell Biol. 131: 575-581, 2009.

NCBI and Uniprot Product Information

• NCBI GI #: 34577059
• NCBI GeneID: 123
• NCBI Accession #: NP_001113.2
• NCBI GenBank Nucleotide #: NM_001122.3
• UniProt Accession #: Q99541; Q9BSC3
• NCBI Official Full Name: perilipin-2
• NCBI Official Synonym Full Names: perilipin 2
• NCBI Official Symbol: PLIN2
• NCBI Official Synonym Symbols: ADFP; ADRP
• NCBI Protein Information: perilipin-2
• UniProt Protein Name: Perilipin-2
• Protein Family: Perilipin
• UniProt Gene Name: PLIN2
• UniProt Synonym Gene Names: ADFP; ADRP

NCBI Description

The protein encoded by this gene belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This protein is associated with the lipid globule surface membrane material, and maybe involved in development and maintenance of adipose tissue. However, it is not restricted to adipocytes as previously thought, but is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells, and tissues, such as lactating mammary gland, adrenal cortex, Sertoli and Leydig cells, and hepatocytes in alcoholic liver cirrhosis, suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2011]

Uniprot Description

perilipin 2: May be involved in development and maintenance of adipose tissue. Belongs to the perilipin family.

Protein type: Endoplasmic reticulum

Chromosomal Location of Human Ortholog: 9p22.1

Cellular Component: endoplasmic reticulum; extracellular region; intracellular membrane-bound organelle; lipid particle; plasma membrane