Western Blot (WB)
(Western blot analysis of Integrin linked ILK expression in rat lung extract (lane 1), mouse liver extract (lane 2) and HELA whole cell lysates (lane 3). Integrin linked ILK at 55KD was detected using rabbit anti- Integrin linked ILK Antigen Affinity purified polyclonal antibody at 0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method. )
Rabbit Integrin linked ILK Polyclonal Antibody | anti-ILK antibody
Anti-Integrin linked ILK Antibody
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Tested Species:In-house tested species with positive results.
Other applications have not been tested.
Western Blot (WB)
(Western blot analysis of Integrin linked ILK expression in rat lung extract (lane 1), mouse liver extract (lane 2) and HELA whole cell lysates (lane 3). Integrin linked ILK at 55KD was detected using rabbit anti- Integrin linked ILK Antigen Affinity purified polyclonal antibody at 0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method. )
Western Blot (WB)
(Western blot analysis of Integrin linked ILK expression in rat lung extract (lane 1), mouse liver extract (lane 2) and HELA whole cell lysates (lane 3). Integrin linked ILK at 55KD was detected using rabbit anti- Integrin linked ILK Antigen Affinity purified polyclonal antibody at 0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method. )
Background: ILK, also known as Integrin-linked kinase, is a serine-threonine protein kinase. Transduction of extracellular matrix signals through integrins influences intracellular and extracellular functions, and appears to require interaction of integrin cytoplasmic domains with cellular proteins. Integrin-linked kinase (ILK) interacts with the cytoplasmic domain of beta-1 integrin. This gene was initially described to encode a serine/ threonine protein kinase with 4 ankyrin-like repeats, which associates with the cytoplasmic domain of beta integrins and acts as a proximal receptor kinase regulating integrin-mediated signal transduction. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. Recent results showed that ILK contains 5 ankyrin-like repeats, and that the C-terminal kinase domain is actually a pseudo-kinase with adaptor function.
2. Fielding AB, Dobreva I, McDonald PC, Foster LJ, Dedhar S (Feb 2008). "Integrin-linked kinase localizes to the centrosome and regulates mitotic spindle organization". The Journal of Cell Biology 180 (4): 681-9.
3. Melchior, C., Kreis, S., Janji, B., Kieffer, N. Promoter characterization and genomic organization of the gene encoding integrin-linked kinase 1. Biochim. Biophys. Acta 1575: 117-122, 2002.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a protein with a kinase-like domain and four ankyrin-like repeats. The encoded protein associates at the cell membrane with the cytoplasmic domain of beta integrins, where it regulates integrin-mediated signal transduction. Activity of this protein is important in the epithelial to mesenchymal transition, and over-expression of this gene is implicated in tumor growth and metastasis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]
Uniprot Description
ILK: a tyrosine kinase-like kinase of the MLK family. Couples integrins and growth factors to downstream pathways involved in cell survival, cell cycle control, cell-cell adhesion and cell motility. Functions as a scaffold bridging the extra-cellular matrix and growth factor receptors to the actin cytoskeleton through interactions with the ILK-PINCH complex. This complex, which includes integrin, PINCH (which links ILK to receptor tyrosine kinases via Nck2), PARVA and affixin, is considered to be one of the convergence points of integrin- and growth factor-signaling pathways. May be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Stimulated downstream of PI3 kinase. Increased expression is correlated with progression of several tumor types, including breast, prostate, and colon carcinomas. Overexpression drives anchorage-independent growth and faster cell cycle.
Protein type: Protein kinase, TKL; Kinase, protein; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); TKL group; MLK family; ILK subfamily
Chromosomal Location of Human Ortholog: 11p15.4
Cellular Component: cell junction; cytoplasm; cytosol; focal adhesion; membrane; nucleoplasm
Molecular Function: protein binding; protein kinase binding; protein serine/threonine kinase activity
Biological Process: cell-matrix adhesion; integrin-mediated signaling pathway; positive regulation of phosphorylation; positive regulation of transcription, DNA-dependent; protein amino acid phosphorylation
Research Articles on ILK
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Product Notes
The ILK ilk (Catalog #AAA178449) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Integrin linked ILK Antibody reacts with Human, Mouse, Rat No cross reactivity with other proteins. and may cross-react with other species as described in the data sheet. AAA Biotech's Integrin linked ILK can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western Blot: 0.1-0.5mug/ml; Tested Species: Human, Mouse, Rat Tested Species:In-house tested species with positive results. Other applications have not been tested. Researchers should empirically determine the suitability of the ILK ilk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Integrin linked ILK, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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