anti-Human, Rat CCN1 Polyclonal Antibody | anti-CCN1 antibody

Anti-CCN1 Antibody

Cat. Num. AAA178382

• Gene Names: CYR61; CCN1; GIG1; IGFBP10
• Reactivity: Human, Rat
• Applications: Western Blot
• Purity: Immunogen Affinity Purified
• Synonyms: CCN1; Polyclonal Antibody; Anti-CCN1 Antibody; Protein CYR61; 3CH61; CCN 1; CCN family member 1; CYR 61; Cyr61; CYR61 protein; CYR61 protein precursor; CYR61_HUMAN; Cysteine rich angiogenic inducer 61; Cysteine rich heparin binding protein 61; Cysteine-rich angiogenic inducer 61; GIG 1; GIG1; GIG1 protein; IBP-10; IGF-binding protein 10; Igfbp 10; IGFBP-10; IGFBP10; Insulin like growth factor binding protein 10; Insulin-like growth factor-binding protein 10; Protein GIG1; cysteine-rich; angiogenic inducer; 61; anti-CCN1 antibody

• Reactivity: Human, Rat
• Clonality: Polyclonal
• Purity/Purification: Immunogen Affinity Purified
• Form/Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 381

• Applicable Applications for anti-CCN1 antibody: Western Blot (WB)
• Application Notes: Western Blot Concentration: 0.1-0.5ug/ml
• Immunogen: E Coli-derived human CCN1 recombinant protein (Position: K54-D381). Human CCN1 shares 91.8% and 92.1% amino acid (aa) sequence identity with mouse and rat CCN1, respectively.
• Ig Type: Rabbit IgG
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti- CCN1 Picoband antibody, MBS178382, Western blottingAll lanes: Anti CCN1 (MBS178382) at 0.5ug/mlLane 1: Rat Thymus Tissue Lysate at 50ugLane 2: Rat Skeletal Muscle Tissue Lysate at 50ugLane 3: A431 Whole Cell Lysate at 40ugLane 4: SGC Whole Cell Lysate at 40ugLane 5: 22RV1 Whole Cell Lysate at 40ugPredicted bind size: 42KDgObserved bind size: 42KD )

Related Product Information for anti-CCN1 antibody

Description: Rabbit IgG polyclonal antibody for Protein CYR61(CYR61) detection. Tested with WB in Human;Rat.

Background: Cysteine-rich angiogenic inducer 61 (CYR61) or CCN family member 1 (CCN1), is a matricellular protein that in humans is encoded by the CYR61 gene. CYR61 is a secreted, extracellular matrix (ECM)-associated signaling protein of the CCN family (CCN intercellular signaling protein). And it is capable of regulating a broad range of cellular activities, including cell adhesion, migration, proliferation, differentiation, apoptosis, and senescence through interaction with cell surface integrin receptors and heparan sulfate proteoglycans. During embryonic development, CYR61 is critical for cardiac septal morphogenesis, blood vessel formation in placenta, and vascular integrity. In adulthood CYR61 plays important roles in inflammation and tissue repair, and is associated with diseases related to chronic inflammation, including rheumatoid arthritis, atherosclerosis, diabetes-related nephropathy and retinopathy, and many different forms of cancers.

References

1. Lau LF (October 2011). "CCN1/CYR61: the very model of a modern matricellular protein". Cell. Mol. Life Sci. 68 (19): 3149-63. 2. Jay P, Berge-Lefranc JL, Marsollier C, Mejean C, Taviaux S, Berta P (May 1997). "The human growth factor-inducible immediate early gene, CYR61, maps to chromosome 1p". Oncogene 14 (14): 1753-7. 3. Jun JI, Lau LF (December 2011). "Taking aim at the extracellular matrix: CCN proteins as emerging therapeutic targets". Nature Reviews Drug Discovery 10 (12): 945-63.

NCBI and Uniprot Product Information

• NCBI GI #: 31542331
• NCBI GeneID: 3491
• NCBI Accession #: NP_001545.2
• NCBI GenBank Nucleotide #: NM_001554.4
• UniProt Accession #: O00622; O14934; O43775; Q9BZL7
• Molecular Weight: 42,027 Da
• NCBI Official Full Name: protein CYR61
• NCBI Official Synonym Full Names: cysteine rich angiogenic inducer 61
• NCBI Official Symbol: CYR61
• NCBI Official Synonym Symbols: CCN1; GIG1; IGFBP10
• NCBI Protein Information: protein CYR61
• UniProt Protein Name: Protein CYR61
• Protein Family: G1/S-specific cyclin
• UniProt Gene Name: CYR61
• UniProt Synonym Gene Names: CCN1; GIG1; IGFBP10; IBP-10; IGF-binding protein 10; IGFBP-10
• UniProt Entry Name: CYR61_HUMAN

NCBI Description

The secreted protein encoded by this gene is growth factor-inducible and promotes the adhesion of endothelial cells. The encoded protein interacts with several integrins and with heparan sulfate proteoglycan. This protein also plays a role in cell proliferation, differentiation, angiogenesis, apoptosis, and extracellular matrix formation. [provided by RefSeq, Sep 2011]

Uniprot Description

CYR61: Promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up- regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5. CYR61-mediated gene regulation is dependent on heparin-binding. Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1. Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-v/beta-5 and cell proliferation through integrin alpha-v/beta-3. Belongs to the CCN family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 1p22.3

Cellular Component: extracellular matrix; proteinaceous extracellular matrix

Molecular Function: extracellular matrix binding; heparin binding; insulin-like growth factor binding; integrin binding

Biological Process: anatomical structure morphogenesis; cell adhesion; cell proliferation; cell-cell signaling; chemotaxis; extracellular matrix organization and biogenesis; intussusceptive angiogenesis; negative regulation of apoptosis; osteoblast differentiation; positive regulation of BMP signaling pathway; positive regulation of caspase activity; positive regulation of cell migration; positive regulation of osteoblast differentiation; positive regulation of osteoblast proliferation; positive regulation of protein amino acid phosphorylation; positive regulation of protein kinase activity; positive regulation of transcription from RNA polymerase II promoter; regulation of cell growth