YB1 Polyclonal Antibody | anti-YB1 antibody

Anti-YB1 Antibody

Cat. Num. AAA178272

• Gene Names: YBX1; YB1; BP-8; CSDB; DBPB; YB-1; CBF-A; CSDA2; EFI-A; NSEP1; NSEP-1; MDR-NF1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen Affinity Purified
• Synonyms: YB1; Polyclonal Antibody; Anti-YB1 Antibody; Nuclease-sensitive element-binding protein 1; BP 8; CBF-A; CCAAT binding transcription factor I subunit A; CCAAT-binding transcription factor I subunit A; CSDA2; CSDB; DBPB; DNA binding protein B; DNA-binding protein B; EFI-A; Enhancer factor I subunit A; MDR NF1; MGC104858; MGC110976; MGC117250; NSEP 1; NSEP1; Nuclease sensitive element binding protein 1; p50; Q15905; Y-box binding protein 1; Y-box transcription factor; Y-box-binding protein 1; YB 1; YB-1; YBOX1_HUMAN; YBX 1; YBX1; Y box binding protein 1; anti-YB1 antibody

• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Immunogen Affinity Purified
• Form/Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 324

• Applicable Applications for anti-YB1 antibody: Western Blot (WB), Immunohistochemistry (IHC) Formalin/Paraffin
• Application Notes: Western Blot Concentration: 0.1-0.5ug/ml; Immunohistochemistry (IHC) Paraffin Concentration: 0.5-1ug/ml; Immunohistochemistry (IHC) Formalin Concentration: 0.5-1ug/ml
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human YB1 (293-320aa ENPKPQDGKETKAADPPAENSSAPEAEQ), identical to the related mouse and rat sequences.
• Ig Type: Rabbit IgG
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti- YB1 Picoband antibody, MBS178272, Western blottingAll lanes: Anti YB1 (MBS178272) at 0.5ug/mlLane 1: Rat Liver Tissue Lysate at 50ugLane 2: Mouse Liver Tissue Lysate at 50ugLane 3: SMMC Whole Cell Lysate at 40ugLane 4: RH35 Whole Cell Lysate at 40ugLane 5: HEPG2 Whole Cell Lysate at 40ugPredicted bind size: 36KDObserved bind size: 50KD )

Immunohistochemistry (IHC)

(Anti- YB1 Picoband antibody, MBS178272,IHC(P)IHC(P): Mouse Testis Tissue )

Immunohistochemistry (IHC)

(Anti- YB1 Picoband antibody, MBS178272,IHC(P)IHC(P): Rat Testis Tissue )

Immunohistochemistry (IHC)

(Anti- YB1 Picoband antibody, MBS178272,IHC(P)IHC(P): Human Lung Cancer Tissue )

Immunohistochemistry (IHC)

(Anti- YB1 Picoband antibody, MBS178272,IHC(F)IHC(F): Human Placenta Tissue )

Related Product Information for anti-YB1 antibody

Description: Rabbit IgG polyclonal antibody for Nuclease-sensitive element-binding protein 1(YBX1) detection. Tested with WB, IHC-P, IHC-F in Human;Mouse;Rat.

Background: YBX1(Y box binding protein 1), commonly referred to as "YB-1" by researchers, is a human protein. YB1 binding has an absolute requirement for the CCAAT box and relative specificity for the Y box. It has a molecular mass of 35,414 and contains 18% basic residues and putative nuclear localization signals. The YBX1 gene is mapped on 1p34.2. Ybx1 was highly expressed in mouse erythroid myeloid lymphoid clone-1 (EML), a hematopoietic precursor cell line, but that it was downregulated in myeloid progenitors and in Gmcsf-treated EML cells during myeloid differentiation. Ybx1 was expressed at high levels in myeloid leukemic cells at different developmental stages. Knockdown of YBX1 in a human leukemic cell line inhibited proliferation ability, induced apoptosis, and induced megakaryocytic differentiation in response to arsenic trioxide treatment. YBX1 is downregulated during myeloid differentiation and aberrant YBX1 expression in leukemic cells may contribute to leukemia development by blocking differentiation.

References

1. Bhullar, J., Sollars, V. E. YBX1 expression and function in early hematopoiesis and leukemic cells. Immunogenetics 63: 337-350, 2011. 2. Fukada, T., Tonks, N. K. Identification of YB-1 as a regulator of PTP1B expression: implications for regulation of insulin and cytokine signaling. EMBO J. 22: 479-493, 2003. 3. Makino, Y., Ohga, T., Toh, S., Koike, K., Okumura, K., Wada, M., Kuwano, M., Kohno, K. Structural and functional analysis of the human Y-box binding protein (YB-1) gene promoter. Nucleic Acid Res. 24: 1873-1878, 1996.

NCBI and Uniprot Product Information

• NCBI GI #: 34098946
• NCBI GeneID: 4904
• NCBI Accession #: NP_004550.2
• NCBI GenBank Nucleotide #: NM_004559.4
• UniProt Accession #: P67809; P16990; P16991; Q14972; Q15325; Q5FVF0
• Molecular Weight: 35,924 Da
• NCBI Official Full Name: nuclease-sensitive element-binding protein 1
• NCBI Official Synonym Full Names: Y-box binding protein 1
• NCBI Official Symbol: YBX1
• NCBI Official Synonym Symbols: YB1; BP-8; CSDB; DBPB; YB-1; CBF-A; CSDA2; EFI-A; NSEP1; NSEP-1; MDR-NF1
• NCBI Protein Information: nuclease-sensitive element-binding protein 1
• UniProt Protein Name: Nuclease-sensitive element-binding protein 1
• UniProt Gene Name: YBX1
• UniProt Synonym Gene Names: NSEP1; YB1; CBF-A; DBPB; EFI-A; YB-1
• UniProt Entry Name: YBOX1_HUMAN

NCBI Description

This gene encodes a highly conserved cold shock domain protein that has broad nucleic acid binding properties. The encoded protein functions as both a DNA and RNA binding protein and has been implicated in numerous cellular processes including regulation of transcription and translation, pre-mRNA splicing, DNA reparation and mRNA packaging. This protein is also a component of messenger ribonucleoprotein (mRNP) complexes and may have a role in microRNA processing. This protein can be secreted through non-classical pathways and functions as an extracellular mitogen. Aberrant expression of the gene is associated with cancer proliferation in numerous tissues. This gene may be a prognostic marker for poor outcome and drug resistance in certain cancers. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on multiple chromosomes. [provided by RefSeq, Sep 2015]

Uniprot Description

YB-1: a nuclear protein that binds to splice sites in pre-mRNA and regulates splice site selection. Binds and stabilizes cytoplasmic mRNA. Contributes to the regulation of translation by modulating the interaction between the mRNA and eukaryotic initiation factors CCAAT-containing Y-box of HLA class II genes. Component of cytoplasmic messenger ribonucleoprotein particles (mRNPs). Interacts with AKT1, SFRS9, THOC4, MSH2, XRCC5, WRN and NCL. Can bind to DNA as a homomeric form, (EFI-A)n or as a heteromeric form in association with EFI-B. Homodimer in the presence of ATP. Involved in cisplatin resistance. Seems to be a negative regulatory factor. May play a role in both transcriptional and translational regulation of spermatogenesis. Found at very low levels at day 10 and levels increase at day 15 and persist throughout adulthood.

Protein type: RNA splicing; RNA processing; Transcription factor; RNA-binding

Chromosomal Location of Human Ortholog: 1p34

Cellular Component: cytoplasm; intracellular membrane-bound organelle; nuclear membrane; nucleoplasm; nucleus; ribonucleoprotein complex; stress granule; U12-dependent spliceosome

Molecular Function: DNA binding; double-stranded DNA binding; GTPase binding; protein binding; RNA binding; single-stranded DNA binding; transcription factor activity

Biological Process: nuclear mRNA splicing, via spliceosome; positive regulation of cell division; positive regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; transcription from RNA polymerase II promoter