anti-Human CD63 Polyclonal Antibody | anti-CD63 antibody

Anti-CD63 Antibody

Cat. Num. AAA178192

• Gene Names: CD63; MLA1; ME491; LAMP-3; OMA81H; TSPAN30
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen Affinity Purified
• Synonyms: CD63; Polyclonal Antibody; Anti-CD63 Antibody; CD63 antigen; Lysosomal associated membrane protein 3; CD 63; CD63 antigen (melanoma 1 antigen); CD63 antigen melanoma 1 antigen; CD63 molecule; CD63_HUMAN; gp55; granulophysin; LAMP 3; LAMP-3; LAMP3; LIMP; Lysosomal-associated membrane protein 3; lysosome associated membrane glycoprotein 3; Mast cell antigen AD1; ME491; melanoma 1 antigen; Melanoma associated antigen ME491; Melanoma associated antigen MLA1; Melanoma-associated antigen ME491; MGC72893; MLA 1; MLA1; NGA; ocular melanoma associated antigen; Ocular melanoma-associated antigen; OMA81H; PTLGP40; Tetraspanin 30; Tetraspanin-30; Tspan 30; Tspan-30; TSPAN30; anti-CD63 antibody

• Reactivity: Human
• Clonality: Polyclonal
• Purity/Purification: Immunogen Affinity Purified
• Form/Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 238

• Applicable Applications for anti-CD63 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Application Notes: Western Blot Concentration: 0.1-0.5ug/ml; Immunohistochemistry (IHC) Paraffin Concentration: 0.5-1ug/ml
• Immunogen: E Coli-derived human CD63 recombinant protein (Position: E97-M238). Human CD63 shares 74% and 73% amino acid (aa) sequence identity with mouse and rat CD63, respectively.
• Ig Type: Rabbit IgG
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti- CD63 Picoband antibody, MBS178192, Western blottingAll lanes: Anti CD63 (MBS178192) at 0.5ug/mlWB: Recombinant Human CD63 Protein 0.5ngPredicted bind size: 26KDObserved bind size: 26KD )

Western Blot (WB)

(Anti- CD63 Picoband antibody, MBS178192, Western blottingAll lanes: Anti CD63 (MBS178192) at 0.5ug/mlWB: HELA Whole Cell Lysate at 40ugPredicted bind size: 26KDObserved bind size: 35KD )

Immunohistochemistry (IHC)

(Anti- CD63 Picoband antibody, MBS178192, IHC(P)IHC(P): Human Intestinal Cancer Tissue )

Related Product Information for anti-CD63 antibody

Description: Rabbit IgG polyclonal antibody for CD63 antigen(CD63) detection. Tested with WB, IHC-P in Human.

Background: CD63 antigen is a protein that in humans is encoded by the CD63 gene. The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. It is mapped to 12q13.2. CD63 is mainly associated with membranes of intracellular vesicles, although cell surface expression may be induced. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. It may function as a blood platelet activation marker. Deficiency of this protein is associated with Hermansky-Pudlak syndrome. Also this gene has been associated with tumor progression.

References

1. "Entrez Gene: CD63 CD63 molecule" 2. Gwynn, B., Eicher, E. M., Peters, L. L. Genetic localization of Cd63, a member of the transmembrane 4 superfamily, reveals two distinct loci in the mouse genome. Genomics 35: 389-391, 1996.

NCBI and Uniprot Product Information

• NCBI GI #: 383872447
• NCBI GeneID: 967
• NCBI Accession #: NP_001244318.1
• NCBI GenBank Nucleotide #: NM_001257389.1
• UniProt Accession #: P08962; Q5TZP3; Q8N6Z9; Q9UCG6; F8VZE2
• Molecular Weight: 17,271 Da
• NCBI Official Full Name: CD63 antigen isoform A
• NCBI Official Synonym Full Names: CD63 molecule
• NCBI Official Symbol: CD63
• NCBI Official Synonym Symbols: MLA1; ME491; LAMP-3; OMA81H; TSPAN30
• NCBI Protein Information: CD63 antigen
• UniProt Protein Name: CD63 antigen
• Protein Family: CD63 antigen
• UniProt Gene Name: CD63
• UniProt Synonym Gene Names: MLA1; TSPAN30; LAMP-3; Tspan-30
• UniProt Entry Name: CD63_HUMAN

NCBI Description

The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. The encoded protein is a cell surface glycoprotein that is known to complex with integrins. It may function as a blood platelet activation marker. Deficiency of this protein is associated with Hermansky-Pudlak syndrome. Also this gene has been associated with tumor progression. Alternative splicing results in multiple transcript variants encoding different protein isoforms. [provided by RefSeq, Apr 2012]

Uniprot Description

CD63: This antigen is associated with early stages of melanoma tumor progression. May play a role in growth regulation. Belongs to the tetraspanin (TM4SF) family.

Protein type: Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 12q12-q13

Cellular Component: cell surface; endosome membrane; extracellular space; integral to plasma membrane; intrinsic to plasma membrane; late endosome membrane; lysosomal membrane; melanosome; plasma membrane; platelet dense granule membrane; protein complex

Molecular Function: protein binding; protein complex binding

Biological Process: cell migration; cell-matrix adhesion; epithelial cell differentiation; pigment granule maturation; platelet degranulation; positive regulation of cell adhesion; positive regulation of receptor internalization; protein transport