Cofilin Polyclonal Antibody | anti-CFL1 antibody

Anti-Cofilin Antibody

Cat. Num. AAA178124

• Gene Names: CFL1; CFL; cofilin; HEL-S-15
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen Affinity Purified
• Synonyms: Cofilin; Polyclonal Antibody; Anti-Cofilin Antibody; Cofilin-1; 18 kDa phosphoprotein; CFL 1; CFL; CFL1; COF1_HUMAN; Cofilin 1; Cofilin 1 non muscle; Cofilin non muscle isoform; epididymis secretory protein Li 15; HEL-S-15; non-muscle isoform; p18; cofilin 1 (non-muscle); anti-CFL1 antibody

• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Immunogen Affinity Purified
• Form/Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 166

• Applicable Applications for anti-CFL1 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Application Notes: Western Blot Concentration: 0.1-0.5ug/ml; Immunohistochemistry (IHC) Paraffin Concentration: 0.5-1ug/ml
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Cofilin (122-152aa DAIKKKLTGIKHELQANCYEEVKDRCTLAEK), identical to the related mouse and rat sequences.
• Ig Type: Rabbit IgG
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti- Cofilin Picoband antibody, MBS178124, Western blottingAll lanes: Anti Cofilin (MBS178124) at 0.5ug/mlLane 1: Rat Testis Tissue Lysate at 50ugLane 2: Rat Lung Tissue Lysate at 50ugLane 3: Rat Ovary Tissue Lysate at 50ugLane 4: Mouse Liver Tissue Lysate at 50ugLane 5: Mouse Brain Tissue Lysate at 50ugLane 6: Mouse Lung Tissue Lysate at 50ugLane 7: HELA Whole Cell Lysate at 40ugLane 8: JURKAT Whole Cell Lysate at 40ugLane 9: A549 Whole Cell Lysate at 40ugLane 10: SMMC Whole Cell Lysate at 40ugPredicted bind size: 19KDObserved bind size: 19KD )

Immunohistochemistry (IHC)

(Anti- Cofilin Picoband antibody, MBS178124, IHC(P)IHC(P): Mouse Intestine Tissue )

Immunohistochemistry (IHC)

(Anti- Cofilin Picoband antibody, MBS178124, IHC(P)IHC(P): Rat Intestine Tissue )

Immunohistochemistry (IHC)

(Anti- Cofilin Picoband antibody, MBS178124, IHC(P)IHC(P): Human Intestinal Cancer Tissue )

Related Product Information for anti-CFL1 antibody

Description: Rabbit IgG polyclonal antibody for Cofilin-1(CFL1) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

Background: Cofilin, a member of the ADF/cofilin family, is actually a protein with 70% sequence homology to ADF, making it part of the ADF/cofilin family of small ADP-binding proteins. It is a ubiquitous actin-binding factor required for the reorganization of actin filaments. The protein is known to sever actin filaments by creating more positive ends on filament fragments, it can cause depolymerization at the minus end of filaments, thereby preventing their reassembly. As a long-lasting in vivo effect, cofilin recycles older ADP-F-actin, helping cell to maintain ATP-G-actin pool for sustained motility. pH, phosphorylation and phosphoinositides regulate cofilin's binding and associating activity with actin.

References

1. Cooper, G. M. and R. E. Hausman. The Cell: A Molecular Approach, 3rd ed. Washington DC: ASM Press 2004 pp.436-440. 2. Ichetovkin I, Han J, Pang KM, Knecht DA, Condeelis JS (April 2000). "Actin filaments are severed by both native and recombinant dictyostelium cofilin but to different extents". Cell Motil. Cytoskeleton 45 (4): 293-306. 3. Lappalainen P, Drubin DG (July 1997). "Cofilin promotes rapid actin filament turnover in vivo".Nature 388 (6637): 78-82.

NCBI and Uniprot Product Information

• NCBI GI #: 5031635
• NCBI GeneID: 1072
• NCBI Accession #: NP_005498.1
• NCBI GenBank Nucleotide #: NM_005507.2
• UniProt Accession #: P23528; Q53Y87; Q9UCA2; B3KUQ1
• Molecular Weight: 18,502 Da
• NCBI Official Full Name: cofilin-1
• NCBI Official Synonym Full Names: cofilin 1
• NCBI Official Symbol: CFL1
• NCBI Official Synonym Symbols: CFL; cofilin; HEL-S-15
• NCBI Protein Information: cofilin-1
• UniProt Protein Name: Cofilin-1
• Protein Family: Cofilin
• UniProt Gene Name: CFL1
• UniProt Synonym Gene Names: CFL; p18
• UniProt Entry Name: COF1_HUMAN

NCBI Description

The protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH-dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM, Apr 2004]

Uniprot Description

Cofilin-1: a cytoskeletal protein that controls actin depolymerization. Has a 5-10-fold higher affinity for ATP-actin monomers than cofilin-1. May promote filament assembly rather than disassembly. Two alternatively spliced variants are described. Isoform b is expressed predominantly in skeletal muscle and heart, while isoform a is expressed in various tissues.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: actin cytoskeleton; cytoplasm; extracellular space; focal adhesion; membrane; nuclear matrix; nucleus; vesicle

Molecular Function: actin binding; protein binding

Biological Process: actin cytoskeleton organization and biogenesis; actin filament depolymerization; cytoskeleton organization and biogenesis; negative regulation of apoptosis; regulation of cell morphogenesis; response to virus; Rho protein signal transduction