Human Tryptase/TPSAB1,B2 PicoKine ELISA Kit | TPSAB1 elisa kit

Human Tryptase/TPSAB1,B2 PicoKine ELISA Kit

Cat. Num. AAA177598

• Gene Names: TPSAB1; TPS1; TPS2; TPSB1
• Reactivity: Human
• Synonyms: Tryptase/TPSAB1; B2 PicoKine; Human Tryptase/TPSAB1; B2 PicoKine ELISA Kit; Tryptase alpha/beta-1/beta-2; Tryptase-1; Tryptase1; Tryptase 1; TPS1; TPS2; TPSB1; Tryptase-2; Tryptase2; Tryptase 2; Tryptase-II; TryptaseII; Tryptase II; TPSB2; Tryptase alpha/beta-1; Tryptase beta-2; tryptase alpha/beta 1/beta 2; TPSAB1 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human Tryptase/TPSAB1,B2
• Sequence Length: 275

• Samples: Cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA)
• Assay Type: Quantitative Sandwich
• Detection Range: 156pg/ml-10000pg/ml
• Sensitivity: <15pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

(Human Tryptase PicoKine ELISA Kit standard curve )

Related Product Information for TPSAB1 elisa kit

Principle of the Assay: The Human Tryptase/TPSAB1,B2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A specific for Tryptase/TPSAB1,B2 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO; Immunogen sequence: M1-V275) and test samples are added to the wells, a biotinylated detection specific for Tryptase/TPSAB1,B2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Tryptase/TPSAB1,B2 amount of sample captured in plate.

Background/Introduction: Tryptases are serine proteases implicated in asthma and are highly expressed in human mast cells. They are derived from at least 4 nonallelic genes clustered on chromosome 16p13.3: TPSAB1, which represents the alpha and beta-I tryptase alleles; TPSB2, which represents the beta-II and beta-III tryptase alleles; TPSG1; and TPSD1. Elevated levels of serum tryptase occur in both anaphylactic and anaphylactoid reactions, but a negative test does not exclude anaphylaxis. Tryptase is less likely to be elevated in food allergy reactions as opposed to other causes of anaphylaxis.

NCBI and Uniprot Product Information

• NCBI GI #: 13775595
• NCBI GeneID: 7177
• NCBI Accession #: NP_003285.2
• NCBI GenBank Nucleotide #: NM_003294.3
• UniProt Accession #: Q15661; P15157; Q15663; Q6B052; Q9H2Y4; Q9H2Y5; Q9UQI1; D2E6R9; D2E6S1
• Molecular Weight: 29,533 Da
• NCBI Official Full Name: tryptase alpha/beta-1
• NCBI Official Synonym Full Names: tryptase alpha/beta 1
• NCBI Official Symbol: TPSAB1
• NCBI Official Synonym Symbols: TPS1; TPS2; TPSB1
• NCBI Protein Information: tryptase alpha/beta-1
• UniProt Protein Name: Tryptase alpha/beta-1
• Protein Family: Tryptase
• UniProt Gene Name: TPSAB1
• UniProt Synonym Gene Names: TPS1; TPS2; TPSB1; Tryptase-1
• UniProt Entry Name: TRYB1_HUMAN

NCBI Description

Tryptases comprise a family of trypsin-like serine proteases, the peptidase family S1. Tryptases are enzymatically active only as heparin-stabilized tetramers, and they are resistant to all known endogenous proteinase inhibitors. Several tryptase genes are clustered on chromosome 16p13.3. These genes are characterized by several distinct features. They have a highly conserved 3' UTR and contain tandem repeat sequences at the 5' flank and 3' UTR which are thought to play a role in regulation of the mRNA stability. These genes have an intron immediately upstream of the initiator Met codon, which separates the site of transcription initiation from protein coding sequence. This feature is characteristic of tryptases but is unusual in other genes. The alleles of this gene exhibit an unusual amount of sequence variation, such that the alleles were once thought to represent two separate genes, alpha and beta 1. Beta tryptases appear to be the main isoenzymes expressed in mast cells; whereas in basophils, alpha tryptases predominate. Tryptases have been implicated as mediators in the pathogenesis of asthma and other allergic and inflammatory disorders. [provided by RefSeq, Jul 2008]

Uniprot Description

TPSAB1: Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. Belongs to the peptidase S1 family. Tryptase subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; EC 3.4.21.59; Secreted, signal peptide; Protease

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: extracellular matrix; extracellular region; extracellular space

Molecular Function: protein binding; serine-type endopeptidase activity; serine-type peptidase activity

Biological Process: defense response; extracellular matrix disassembly; proteolysis