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Typical Testing Data/Standard Curve (for reference only)

Mouse SAA ELISA Kit | SAA1 elisa kit

Mouse SAA PicoKine ELISA Kit

Reactivity
Mouse
Synonyms
SAA; Mouse SAA PicoKine ELISA Kit; Serum amyloid A protein; serum amyloid A1; Amyloid fibril protein AA; Amyloid protein A; MGC111216; OTTMUSP00000045357; PIG4; SAA1; Serum amyloid A 1; Serum amyloid A 1 protein; serum amyloid A protein; Serum amyloid A protein precursor; Serum amyloid A1 isoform 1; Serum amyloid A1 isoform 2; TP53I4; Tumor protein p53 inducible protein 4; SAA1 elisa kit
Ordering
For Research Use Only!
Reactivity
Mouse
Specificity
Natural and recombinant Mouse SAA/SAA1
Samples
Serum, plasma(heparin, EDTA) and urine
Assay Type
Quantitative Sandwich
Detection Range
1.56ng/ml-100ng/ml
Sensitivity
<150pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for SAA1 elisa kit
Principle of the Assay: The Mouse SAA/SAA1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for SAA/SAA1 has been precoated onto 96-well plates. Standards(Expression system for standard: E.coli; Immunogen sequence: G20-Y122) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for SAA/SAA1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse SAA/SAA1 amount of sample captured in plate.

Background/Introduction: Serum amyloid A protein(SAA), also known as SAA1, is a protein that in humans is encoded by the SAA1 gene. This gene encodes a member of the serum amyloid A family of apolipoproteins. It is mapped to 11p15.1. SAA is a major acute phase protein that is highly expressed in response to inflammation and tissue injury. This protein also plays an important role in HDL metabolism and cholesterol homeostasis. High levels of this protein are associated with chronic inflammatory diseases including atherosclerosis, rheumatoid arthritis, Alzheimer's disease and Crohn's disease. This protein may also be a potential biomarker for certain tumors.

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Product Notes

The Mouse SAA1 (Catalog #AAA177310) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA177310 ELISA Kit recognizes Mouse SAA1. It is sometimes possible for the material contained within the vial of "SAA, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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