Mouse FGF1 ELISA Kit | FGF1 elisa kit

Mouse FGF1 PicoKine ELISA Kit

Cat. Num. AAA177264

• Gene Names: Fgf1; Fam; Fgfa; Dffrx; Fgf-1
• Reactivity: Mouse
• Synonyms: FGF1; Mouse FGF1 PicoKine ELISA Kit; Fibroblast growth factor 1; fibroblast growth factor 1 (acidic); Acidic fibroblast growth factor; aFGF; ECGF; Endothelial cell growth factor; FGF-1; Fgf1; FGF1_HUMAN; FGFA; HBGF-1; Heparin-binding growth factor 1; FGF1 elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant mouse FGF1
• Sequence Length: 155

• Samples: Cell culture supernates, serum and plasma (heparin, EDTA)
• Assay Type: Sandwich
• Detection Range: 31.2pg/ml-2000pg/ml
• Sensitivity: < 10pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Mouse FGF1 PicoKine ELISA Kit standard curve )

Testing Data

(Applications: ELISASample: Skin tissue homogenatesDetection: --"This product was used to investigate the dynamic change of FGF1 during mouse skin wound healing. The reasons to choose this product are: a) Easy to use. b) Less expense, c) Standard covers a wide range of concentrations, d) It works for tissue homogenates. FGF1 significantly increased at the proliferation/remolding stage. Easy to use and less expensive. Highly recommended.")

Related Product Information for FGF1 elisa kit

Principle of the assay: mouse FGF1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for FGF1 has been precoated onto 96-well plates. Standards(E.coli, F16-D155) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for FGF1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse FGF1 amount of sample captured in plate.
Background: Heparin-binding growth factor 1 is a protein that in humans is encoded by the FGF1 gene. The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. The FGF1 gene was mapped to chromosome 5q31.3-q33.2 by in situ hybridization.

NCBI and Uniprot Product Information

• NCBI GI #: 47117679
• NCBI GeneID: 14164
• UniProt Accession #: P61148; P10935
• Molecular Weight: 17,418 Da
• NCBI Official Full Name: Fibroblast growth factor 1
• NCBI Official Synonym Full Names: fibroblast growth factor 1
• NCBI Official Symbol: Fgf1
• NCBI Official Synonym Symbols: Fam; Fgfa; Dffrx; Fgf-1
• NCBI Protein Information: fibroblast growth factor 1; aFGF; HBGF-1; acidic fibroblast growth factor; heparin-binding growth factor 1; fibroblast growth factor 1 (acidic)
• UniProt Protein Name: Fibroblast growth factor 1
• Protein Family: Fibroblast growth factor
• UniProt Gene Name: Fgf1
• UniProt Synonym Gene Names: Fgf-1; Fgfa; FGF-1; aFGF; HBGF-1
• UniProt Entry Name: FGF1_MOUSE

Uniprot Description

FGF1: Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Monomer. Homodimer. Interacts with FGFR1, FGFR2, FGFR3 and FGFR4. Affinity between fibroblast growth factors (FGFs) and their receptors is increased by heparan sulfate glycosaminoglycans that function as coreceptors. Found in a complex with FGFBP1, FGF1 and FGF2. Interacts with FGFBP1. Part of a Cu(2+)-dependent multiprotein aggregate containing FGF1, S100A13 and SYT1. Interacts with SYT1. Interacts with S100A13. Belongs to the heparin-binding growth factors family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytokine; Cell development/differentiation; Motility/polarity/chemotaxis

Cellular Component: nucleoplasm; extracellular space; proteinaceous extracellular matrix; cytoplasm; nucleolus; extracellular region; cytosol; nucleus

Molecular Function: heparin binding; protein binding; growth factor activity; Hsp70 protein binding; fibroblast growth factor receptor binding; receptor binding

Biological Process: fibroblast growth factor receptor signaling pathway; positive regulation of transcription, DNA-dependent; multicellular organismal development; positive regulation of cholesterol biosynthetic process; cardiac muscle cell proliferation; cell proliferation; positive regulation of MAP kinase activity; positive regulation of angiogenesis; induction of an organ; positive regulation of cell division; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; angiogenesis; positive regulation of protein amino acid phosphorylation; cell differentiation; positive regulation of epithelial cell proliferation; positive regulation of cell migration; lung development