Human ADAMTS1 ELISA Kit | ADAMTS1 elisa kit

Human ADAMTS1 PicoKine ELISA Kit

Cat. Num. AAA177144

• Gene Names: ADAMTS1; C3-C5; METH1
• Reactivity: Human
• Synonyms: ADAMTS1; Human ADAMTS1 PicoKine ELISA Kit; A disintegrin and metalloproteinase with thrombospondin motifs 1; ADAM metallopeptidase with thrombospondin type 1 motif; 1; A disintegrin like and metalloprotease with thrombospondin type 1 motif 1; A disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 1; ADAM metallopeptidase with thrombospondin type 1 motif 1; ADAM TS 1; ADAM TS1; ADAM-TS 1; ADAM-TS1; ADAMTS 1; ADAMTS; ADAMTS-1; Adamts1; ATS1_HUMAN; C3 C5; KIAA1346; METH 1; METH-1; METH1; ADAMTS1 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human ADAMTS1
• Sequence Length: 967

• Samples: Cell culture supernates, serum and plasma(heparin).
• Assay Type: Sandwich
• Detection Range: 93.8pg/ml-6000pg/ml
• Sensitivity: <10pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Human ADAMTS1 PicoKine ELISA Kit standard curve )

Related Product Information for ADAMTS1 elisa kit

Principle of the Assay: Human ADAMTS1 ELISA Kit was based on standard sandwich enzyme-linked immunesorbent assay technology. A monoclonal antibody from mouse specific for ADAMTS1 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO; Immunogen sequence: F253-A734) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ADAMTS1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human ADAMTS1 amount of sample captured in plate.

Background/Introduction: ADAMTS1 (A Disintegrin-Like and Metalloproteinase with Thrombospondin Type 1 Motif, 1), also known as METH1, is an enzyme that in humans is encoded by the ADAMTS1 gene. ADAMTS is a family of proteins believed to be anchored to the extracellular matrix (ECM) through interactions with aggregan or other matrix components by one or more thrombospondin type 1 motifs. ADAMTS1 gene is mapped to 21q21.2 based on sequence similarity between the ADAMTS1 sequence and a chromosome 21q21.2 clone. It has been found that ADAMTS1 can disrupt angiogenesis in vivo and in vitro more efficiently than ADAMTS8, THBS1, or endostatin. What's more, ADAMTS1 disrupts angiogenesis in vivo and in vitro more efficiently than ADAMTS8, THBS1, or endostatin.

NCBI and Uniprot Product Information

• NCBI GI #: 124053460
• NCBI GeneID: 9510
• UniProt Accession #: Q9UHI8; Q9NSJ8; Q9P2K0; Q9UH83; Q9UP80; D3DSD5
• Molecular Weight: 105,358 Da
• NCBI Official Full Name: A disintegrin and metalloproteinase with thrombospondin motifs 1
• NCBI Official Synonym Full Names: ADAM metallopeptidase with thrombospondin type 1 motif, 1
• NCBI Official Symbol: ADAMTS1
• NCBI Official Synonym Symbols: C3-C5; METH1
• NCBI Protein Information: A disintegrin and metalloproteinase with thrombospondin motifs 1; METH-1; ADAM-TS1; ADAMTS-1; ADAM-TS 1; human metalloproteinase with thrombospondin type 1 motifs; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 1
• UniProt Protein Name: A disintegrin and metalloproteinase with thrombospondin motifs 1
• Protein Family: A disintegrin and metalloproteinase with thrombospondin motifs
• UniProt Gene Name: ADAMTS1
• UniProt Synonym Gene Names: KIAA1346; METH1; ADAM-TS 1; ADAM-TS1; ADAMTS-1
• UniProt Entry Name: ATS1_HUMAN

NCBI Description

This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motif) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The protein encoded by this gene contains two disintegrin loops and three C-terminal TS motifs and has anti-angiogenic activity. The expression of this gene may be associated with various inflammatory processes as well as development of cancer cachexia. This gene is likely to be necessary for normal growth, fertility, and organ morphology and function. [provided by RefSeq, Jul 2008]

Uniprot Description

ADAMTS1: Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. Has angiogenic inhibitor activity. Active metalloprotease, which may be associated with various inflammatory processes as well as development of cancer cachexia. May play a critical role in follicular rupture.

Protein type: Secreted, signal peptide; EC 3.4.24.-; Secreted; Protease; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 21q21.2

Cellular Component: cytoplasm; basement membrane; cytoplasmic vesicle

Molecular Function: heparin binding; metallopeptidase activity; zinc ion binding; metalloendopeptidase activity

Biological Process: integrin-mediated signaling pathway; negative regulation of cell proliferation; kidney development; ovulation from ovarian follicle; proteolysis