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Immunocytochemistry (ICC)
(Anti-HSD17B1 antibody, MBS177074, ICCICC: HELA Cell )
Rabbit HSD17B1 Polyclonal Antibody | anti-HSD17B1 antibody
Anti-HSD17B1 antibody
Gene Names
HSD17B1; HSD17; EDHB17; EDH17B2; SDR28C1
Reactivity
Reacts with: Human, RatPredicted Species: Mouse
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry
Purity
Immunogen affinity purified.
Synonyms
HSD17B1; Polyclonal Antibody; Anti-HSD17B1 antibody; Estradiol 17-beta-dehydrogenase 1; hydroxysteroid(17-beta) dehydrogenase 1; 17 beta HSD 1 antibody; 17 beta hydroxysteroid dehydrogenase type 1 antibody; 17-beta-HSD 1 antibody; 17-beta-hydroxysteroid dehydrogenase type 1 antibody; 20 alpha-hydroxysteroid dehydrogenase antibody; 20-alpha-HSD antibody; DHB1_HUMAN antibody; E17KSR antibody; E2DH antibody; EDH17B1 antibody; EDH17B2 antibody; EDHB17 antibody; Estradiol 17 beta dehydrogenase 1 antibody; Estradiol 17-beta-dehydrogenase 1 antibody; HSD17 antibody; HSD17B1 antibody; Hydroxysteroid(17 beta) dehydrogenase 1 antibody; MGC13814 antibody; Placental 17 beta hydroxysteroid dehydrogenase antibody; Placental 17-beta-hydroxysteroid dehydrogenase antibody; SDR28C1 antibody; Short chain dehydrogenase/reductase family 28CE; member 1 antibody; anti-HSD17B1 antibody
Host
Rabbit
Reactivity
Reacts with: Human, Rat
Predicted Species: Mouse
Predicted Species: Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
No Cross reactivity with other proteins.
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Sequence Length
328
Applicable Applications for anti-HSD17B1 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC)
Application Notes
Western Blot:
Concentration: 0.1-0.5ug/ml
Tested Species: Hu, Rat
Predicted Species: Ms
Immunohistochemistry (Paraffin-embedded Section):
Concentration: 0.5-1ug/ml
Tested Species: Hu, Rat
Predicted Species: Ms
Antigen Retrieval: By Heat
Immunocytochemistry:
Concentration: 0.5-1ug/ml
Tested Species: Hu
Predicted Species: Rat, Ms
Tested Species: In-house tested species with positive results.
Predicted Species: Species predicted to be fit for the product based on sequence similarities.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Optimal dilutions should be determined by end users.
Concentration: 0.1-0.5ug/ml
Tested Species: Hu, Rat
Predicted Species: Ms
Immunohistochemistry (Paraffin-embedded Section):
Concentration: 0.5-1ug/ml
Tested Species: Hu, Rat
Predicted Species: Ms
Antigen Retrieval: By Heat
Immunocytochemistry:
Concentration: 0.5-1ug/ml
Tested Species: Hu
Predicted Species: Rat, Ms
Tested Species: In-house tested species with positive results.
Predicted Species: Species predicted to be fit for the product based on sequence similarities.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Optimal dilutions should be determined by end users.
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human HSD17B1 (29-43aa QSFKVYATLRDLKTQ), different from the related rat and mouse sequences by one amino acid.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Immunocytochemistry (ICC)
(Anti-HSD17B1 antibody, MBS177074, ICCICC: HELA Cell )
Immunocytochemistry (ICC)
(Anti-HSD17B1 antibody, MBS177074, ICCICC: HELA Cell )
Western Blot (WB)
(Anti-HSD17B1 antibody, MBS177074, Western blottingLane 1: Rat Kidney Tissue LysateLane 2: Rat Liver Tissue LysateLane 3: 293T Cell LysateLane 4: HELA Cell Lysate )
Western Blot (WB)
(Anti-HSD17B1 antibody, MBS177074, Western blottingLane 1: Rat Kidney Tissue LysateLane 2: Rat Liver Tissue LysateLane 3: 293T Cell LysateLane 4: HELA Cell Lysate )
Immunohistochemistry (IHC)
(Anti-HSD17B1 antibody, MBS177074, IHC(P)IHC(P): Rat Liver Tissue )
Immunohistochemistry (IHC)
(Anti-HSD17B1 antibody, MBS177074, IHC(P)IHC(P): Rat Liver Tissue )
Immunohistochemistry (IHC)
(Anti-HSD17B1 antibody, MBS177074, IHC(P)IHC(P): Rat Ovary Tissue )
Immunohistochemistry (IHC)
(Anti-HSD17B1 antibody, MBS177074, IHC(P)IHC(P): Rat Ovary Tissue )
Immunohistochemistry (IHC)
(Anti-HSD17B1 antibody, MBS177074, IHC(P)IHC(P): Human Placenta Tissue )
Immunohistochemistry (IHC)
(Anti-HSD17B1 antibody, MBS177074, IHC(P)IHC(P): Human Placenta Tissue )
Related Product Information for anti-HSD17B1 antibody
Description: Rabbit IgG polyclonal antibody for Estradiol 17-beta-dehydrogenase 1(HSD17B1) detection. Tested with WB, IHC-P, ICC in Human, Mouse, Rat.
Background: HSD17B1(17-BETA-HYDROXYSTEROID DEHYDROGENASE I), also known as 17-beta-hydroxysteroid dehydrogenase or 17-ketosteroid reductase, is an enzyme that is responsible for the interconversion of estrone(E1) and estradiol(E2) as well as the interconversion of androstenedione and testosterone. Its cytogenetic location is 17q21.2. HSD17B1 gene expression is detected in 34 of 42(81%) adenomas in all tumor subtypes; HSD17B2 mRNA is detected in 18 of 42(43%) adenomas but not in prolactinomas. Human estrogenic HSD17B1 is an NADP(H)-preferring enzyme. It possesses 11- and 4-fold higher specificity toward NADP(H) over NAD(H) for oxidation and reduction, respectively, as demonstrated by kinetic studies. All 4 HSD17B isoforms are variably expressed in human anterior pituitary adenomas, which also show HSD17B enzyme activity, suggesting that HSD17B may play an important role in regulating the local cellular levels of estradiol.
Background: HSD17B1(17-BETA-HYDROXYSTEROID DEHYDROGENASE I), also known as 17-beta-hydroxysteroid dehydrogenase or 17-ketosteroid reductase, is an enzyme that is responsible for the interconversion of estrone(E1) and estradiol(E2) as well as the interconversion of androstenedione and testosterone. Its cytogenetic location is 17q21.2. HSD17B1 gene expression is detected in 34 of 42(81%) adenomas in all tumor subtypes; HSD17B2 mRNA is detected in 18 of 42(43%) adenomas but not in prolactinomas. Human estrogenic HSD17B1 is an NADP(H)-preferring enzyme. It possesses 11- and 4-fold higher specificity toward NADP(H) over NAD(H) for oxidation and reduction, respectively, as demonstrated by kinetic studies. All 4 HSD17B isoforms are variably expressed in human anterior pituitary adenomas, which also show HSD17B enzyme activity, suggesting that HSD17B may play an important role in regulating the local cellular levels of estradiol.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
34,950 Da
NCBI Official Full Name
Estradiol 17-beta-dehydrogenase 1
NCBI Official Synonym Full Names
hydroxysteroid (17-beta) dehydrogenase 1
NCBI Official Symbol
HSD17B1
NCBI Official Synonym Symbols
HSD17; EDHB17; EDH17B2; SDR28C1
NCBI Protein Information
estradiol 17-beta-dehydrogenase 1; E2DH; 20-alpha-HSD; 17-beta-HSD 1; estradiol 17-beta-dehydrogenase-1; 20 alpha-hydroxysteroid dehydrogenase; 17-beta-hydroxysteroid dehydrogenase type 1; placental 17-beta-hydroxysteroid dehydrogenase; hydroxysteroid (17-beta) dehydrogenase 1 isoform; short chain dehydrogenase/reductase family 28CE, member 1
UniProt Protein Name
Estradiol 17-beta-dehydrogenase 1
Protein Family
UniProt Gene Name
HSD17B1
UniProt Synonym Gene Names
E17KSR; EDH17B1; EDH17B2; EDHB17; 17-beta-HSD 1; 20-alpha-HSD
UniProt Entry Name
DHB1_HUMAN
Uniprot Description
HSD17B1: Favors the reduction of estrogens and androgens. Also has 20-alpha-HSD activity. Uses preferentially NADH. Belongs to the short-chain dehydrogenases/reductases (SDR) family.
Protein type: Lipid Metabolism - androgen and estrogen; EC 1.1.1.62; Oxidoreductase
Chromosomal Location of Human Ortholog: 17q11-q21
Cellular Component: nucleoplasm; nuclear membrane; cytoplasm; cytosol
Molecular Function: estradiol 17-beta-dehydrogenase activity; catalytic activity; 3-alpha(17-beta)-hydroxysteroid dehydrogenase (NAD+) activity
Biological Process: steroid metabolic process; estrogen metabolic process; steroid biosynthetic process; estrogen biosynthetic process
Research Articles on HSD17B1
Similar Products
Product Notes
The HSD17B1 hsd17b1 (Catalog #AAA177074) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-HSD17B1 antibody reacts with Reacts with: Human, Rat Predicted Species: Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's HSD17B1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC). Western Blot: Concentration: 0.1-0.5ug/ml Tested Species: Hu, Rat Predicted Species: Ms Immunohistochemistry (Paraffin-embedded Section): Concentration: 0.5-1ug/ml Tested Species: Hu, Rat Predicted Species: Ms Antigen Retrieval: By Heat Immunocytochemistry: Concentration: 0.5-1ug/ml Tested Species: Hu Predicted Species: Rat, Ms Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20 mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Researchers should empirically determine the suitability of the HSD17B1 hsd17b1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HSD17B1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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