Rabbit anti-Human, Mouse Siglec 5 Polyclonal Antibody | anti-SIGLEC5 antibody

Siglec 5, CT (Sialic Acid-binding Ig-like Lectin 5, Siglec 5, Obesity-binding Protein 2, OB-binding Protein 2, OB-BP2, CD33 Antigen-like 2, CD170, CD33L2, OBBP2) (Azide free) (HRP)

Cat. Num. AAA6499379

• Gene Names: SIGLEC5; CD170; OBBP2; CD33L2; OB-BP2; SIGLEC-5
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: Siglec 5; Polyclonal Antibody; CT (Sialic Acid-binding Ig-like Lectin 5; Obesity-binding Protein 2; OB-binding Protein 2; OB-BP2; CD33 Antigen-like 2; CD170; CD33L2; OBBP2) (Azide free) (HRP); anti-SIGLEC5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human SIGLEC5. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
• Sequence Length: 551

• Applicable Applications for anti-SIGLEC5 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-1:500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human SIGLEC5 (KLH).
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(The anti-Siglec5 C-term Pab is used in Western blot to detect Siglec5 in mouse liver tissue lysate.)

Western Blot (WB)

(SIGLEC5 Antibody western blot analysis in SK-BR-3 cell line lysates (35ug/lane).This demonstrates the SIGLEC5 antibody detected the SIGLEC5 protein (arrow).)

Product Categories/Family for anti-SIGLEC5 antibody

Antibodies; Lectins

References

1. Erickson-Miller, C.L., et al., Exp. Hematol. 31(5):382-388 (2003). 2. Patel, N., et al., J. Biol. Chem. 274(32):22729-22738 (1999). 3. Cornish, A.L., et al., Blood 92(6):2123-2132 (1998). 4. Kim, H.S., Cytogenet. Cell Genet. 84(1-2), 96 (1999).

NCBI and Uniprot Product Information

• NCBI GI #: 5759106
• NCBI GeneID: 8778
• Molecular Weight: 60.715
• NCBI Official Full Name: sialic acid binding Ig-like lectin-5
• NCBI Official Synonym Full Names: sialic acid binding Ig like lectin 5
• NCBI Official Symbol: SIGLEC5
• NCBI Official Synonym Symbols: CD170; OBBP2; CD33L2; OB-BP2; SIGLEC-5
• NCBI Protein Information: sialic acid-binding Ig-like lectin 5
• Protein Family: Sialic acid-binding Ig-like lectin

NCBI Description

This gene encodes a member of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. These cell surface lectins are characterized by structural motifs in the immunoglobulin (Ig)-like domains and sialic acid recognition sites in the first Ig V set domain. The encoded protein is a member of the CD33-related subset of Siglecs and inhibits the activation of several cell types including monocytes, macrophages and neutrophils. Binding of group B Streptococcus (GBS) to the encoded protein plays a role in GBS immune evasion. [provided by RefSeq, Feb 2012]