Rabbit PIAS1 Polyclonal Antibody | anti-PIAS1 antibody

Anti-PIAS1 antibody

Cat. Num. AAA177040

• Gene Names: PIAS1; GBP; ZMIZ3; DDXBP1; GU/RH-II
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry
• Purity: Immunogen affinity purified.
• Synonyms: PIAS1; Polyclonal Antibody; Anti-PIAS1 antibody; E3 SUMO-protein ligase PIAS1; protein inhibitor of activated STAT; 1; DDXBP1 antibody; DEAD/H box binding protein 1 antibody; DEAD/H box-binding protein 1 antibody; E3 SUMO-protein ligase PIAS1 antibody; GBP antibody; Gu binding protein antibody; Gu-binding protein antibody; Pias1 antibody; PIAS1_HUMAN antibody; Protein inhibitor of activated STAT protein 1 antibody; RNA helicase II binding protein antibody; RNA helicase II-binding protein antibody; anti-PIAS1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized
• Sequence Length: 651

• Applicable Applications for anti-PIAS1 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC)
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human PIAS1 (636-651aa DTASIFGIIPDIISLD), identical to the related mouse and rat sequences.
• Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Immunocytochemistry (ICC)

(Anti-PIAS1 antibody, MBS177040, ICCICC: A549 Cell )

Immunohistochemistry (IHC)

(Anti-PIAS1 antibody, MBS177040, IHC(P)IHC(P): Rat Testis Tissue )

Immunohistochemistry (IHC)

(Anti-PIAS1 antibody, MBS177040, IHC(P)IHC(P): Rat Brain Tissue )

Immunohistochemistry (IHC)

(Anti-PIAS1 antibody, MBS177040, IHC(P)IHC(P): Rat Brain Tissue )

Immunohistochemistry (IHC)

(Anti-PIAS1 antibody, MBS177040, IHC(P)IHC(P): Human Placenta Tissue )

Western Blot (WB)

(Anti-PIAS1 antibody, MBS177040, All Western blottingAll lanes: Anti-PIAS1(MBS177040) at 0.5ug/mlLane 1: Rat Testis Tissue Lysate at 40ugLane 2: Mouse Testis Tissue Lysate at 40ugLane 3: HELA Whole Cell Lysate at 40ugLane 4: JURKAT Whole Cell Lysate at 40ugLane 5: MCF-7 Whole Cell Lysate at 40ugLane 6: SKOV Whole Cell Lysate at 40ugPredicted bind size: 72KDObserved bind size: 100KD )

Related Product Information for anti-PIAS1 antibody

Description: Rabbit IgG polyclonal antibody for E3 SUMO-protein ligase PIAS1(PIAS1) detection. Tested with WB, IHC-P, ICC in Human, Mouse, Rat.
Background: E3 SUMO-protein ligase PIAS1 is an enzyme that in humans is encoded by the PIAS1 gene. It is mapped to 15q23. This gene encodes a member of the mammalian PIAS [protein inhibitor of activated STAT-1(signal transducer and activator of transcription-1)] family. This member contains a putative zinc-binding motif and a highly acidic region. PIAS1 inhibited STAT1-mediated gene activation in response to interferon when expressed in mammalian cells. It functions in testis as a nuclear receptor transcriptional coregulator and may have a role in AR initiation and maintenance of spermatogenesis. On the other hand, PIAS1 also can function as a SUMO ligase, or possibly as a tightly bound regulator of it, toward p53.

NCBI and Uniprot Product Information

• NCBI GI #: 20138891
• NCBI GeneID: 8554
• UniProt Accession #: O75925; Q147X4; Q99751; Q9UN02; B2RB67; B3KSY9
• Molecular Weight: 72,203 Da
• NCBI Official Full Name: E3 SUMO-protein ligase PIAS1
• NCBI Official Synonym Full Names: protein inhibitor of activated STAT, 1
• NCBI Official Symbol: PIAS1
• NCBI Official Synonym Symbols: GBP; ZMIZ3; DDXBP1; GU/RH-II
• NCBI Protein Information: E3 SUMO-protein ligase PIAS1; gu-binding protein; AR interacting protein; DEAD/H box-binding protein 1; RNA helicase II-binding protein; zinc finger, MIZ-type containing 3; protein inhibitor of activated STAT-1; protein inhibitor of activated STAT protein 1; DEAD/H (Asp-Glu-Ala-Asp/His) box binding protein 1
• UniProt Protein Name: E3 SUMO-protein ligase PIAS1
• Protein Family: E3 SUMO-protein ligase
• UniProt Gene Name: PIAS1
• UniProt Synonym Gene Names: DDXBP1; GBP
• UniProt Entry Name: PIAS1_HUMAN

NCBI Description

This gene encodes a member of the mammalian PIAS [protein inhibitor of activated STAT-1 (signal transducer and activator of transcription-1)] family. This member contains a putative zinc-binding motif and a highly acidic region. It inhibits STAT1-mediated gene activation and the DNA binding activity, binds to Gu protein/RNA helicase II/DEAD box polypeptide 21, and interacts with androgen receptor (AR). It functions in testis as a nuclear receptor transcriptional coregulator and may have a role in AR initiation and maintenance of spermatogenesis. [provided by RefSeq, Jul 2008]

Uniprot Description

PIAS1: Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. In vitro, binds A/T-rich DNA. The effects of this transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context. Together with PRMT1, may repress STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling. Interacts with NCOA2 and AR. Interacts with NR2C1; the interaction promotes its sumoylation. Interacts with DDX21, CSRP2, AXIN1, JUN, UBE2I, SUMO1, SATB2, PLAG1, TP53 and STAT1 (dimer), following IFNA1-stimulation. Interacts with SP3 (preferentially when SUMO-modified). Interacts with KLF8; the interaction results in SUMO ligation and repression of KLF8 transcriptional activity and of its cell cycle progression into G(1) phase. Interacts with STAT1. Interacts with CHUK/IKKA; this interaction induces PIAS1 phosphorylation. Interacts with PTK2/FAK1; the interaction promotes its sumoylation. Interacts with DDX5. Expressed in numerous tissues with highest level in testis. Belongs to the PIAS family.

Protein type: EC 6.3.2.-; Transcription, coactivator/corepressor; SUMO conjugating system; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 15q

Cellular Component: nucleoplasm; PML body; nuclear speck; nucleus

Molecular Function: protein domain specific binding; protein binding; enzyme binding; DNA binding; androgen receptor binding; zinc ion binding; ubiquitin protein ligase binding; transcription coactivator activity; transcription corepressor activity; ligase activity

Biological Process: positive regulation of protein sumoylation; protein sumoylation; transcription, DNA-dependent; positive regulation of smooth muscle cell differentiation; positive regulation of transcription, DNA-dependent; cytokine and chemokine mediated signaling pathway; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; androgen receptor signaling pathway; spermatogenesis; negative regulation of transcription from RNA polymerase II promoter; JAK-STAT cascade; protein-DNA complex assembly; regulation of cell proliferation