SDS-PAGE Loading Buffer (5x) Western Blot Related Reagent
SDS-PAGE Protein Loading Buffer 5X (Reducing)
Synonyms
SDS-PAGE Loading Buffer (5x); SDS-PAGE Protein Loading Buffer 5X (Reducing); SDS-PAGE Loading Buffer (5x) western blot related reagent
Form/Format
Liquid; Contents 10% SDS, 500mM DTT, 50% Glycerol, 250mM Tris-HCL and 0.5% bromophenol blue dye, PH6.8.
Assay Protocol
1. Equilibrate SDS-PAGE Protein Loading Buffer 5X to room temperature or thaw Loading Buffer in a water bath no higher than 30°C.
2. Mix one volume of SDS-PAGE Protein Loading Buffer 5X with four volume of protein sample (i.e. add 4mL protein sample into 1 mL Loading Buffer).
3. Boil sample for 3-5 min.
Note: If semitransparent viscous substance remains after boiling, boil sample for another 5-10 min or add 1X SDS-PAGE Protein Loading Buffer which is diluted from 5X SDS-PAGE Protein Loading Buffer to the sample and then boil for another 3-5 min.
4. Allow sample to cool to room temperature.
5. Load sample into the wells of the SDS-PAGE gel and begin electrophoresis.
6. Stop electrophoresis when bromophenol blue dye front reaches to the bottom of the gel.
2. Mix one volume of SDS-PAGE Protein Loading Buffer 5X with four volume of protein sample (i.e. add 4mL protein sample into 1 mL Loading Buffer).
3. Boil sample for 3-5 min.
Note: If semitransparent viscous substance remains after boiling, boil sample for another 5-10 min or add 1X SDS-PAGE Protein Loading Buffer which is diluted from 5X SDS-PAGE Protein Loading Buffer to the sample and then boil for another 3-5 min.
4. Allow sample to cool to room temperature.
5. Load sample into the wells of the SDS-PAGE gel and begin electrophoresis.
6. Stop electrophoresis when bromophenol blue dye front reaches to the bottom of the gel.
Assay Principle
Using bromophenol blue dye, SDS-PAGE Protein Loading Buffer is a ready-to-use 5X solution. It contains 10% SDS, 500Mm DTT, 50% Glycerol, 500mM Tris-HCL and 0.5% bromophenol blue dye. It can be used for SDS-PAGE protein loading of conventional proteins. It is especially formulated for protein sample preparation to be used in the Laemmli SDS-PAGE system. It is sufficient to prepare 15 mL protein samples. And the whole sample preparation process can be finished within 5 minutes.
Notes
1. SDS-PAGE Protein Loading Buffer 5X contains DTT which has a slightly irritating odor.
2. SDS-PAGE Protein Loading Buffer 5X should be completely dissolved before use.
3. Please wear the lab coat and disposable gloves and operate in a laboratory hood.
2. SDS-PAGE Protein Loading Buffer 5X should be completely dissolved before use.
3. Please wear the lab coat and disposable gloves and operate in a laboratory hood.
Equivalent
GeneCopoeia (Please inquire)
Preparation and Storage
Upon receipt store at -20°C. SDS-PAGE Protein Loading Buffer 5X (Reducing) is stable for one year. Product is shipped on ice.
Related Product Information for SDS-PAGE Loading Buffer (5x) western blot related reagent
SDS PAGE Sample Buffer 5X (Reducing) is the most commonly used sample buffer for Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis (SDS-PAGE) of denatured proteins in the Laemmli SDS-PAGE system.
Product Categories/Family for SDS-PAGE Loading Buffer (5x) western blot related reagent
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Product Notes
The SDS-PAGE Loading Buffer (5x) (Catalog #AAA176755) is a Western Blot Related Reagent and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "SDS-PAGE Loading Buffer (5x), Western Blot Related Reagent" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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