Biotin Conjugated Goat Anti-human IgM Secondary Antibody
Biotin Conjugated Goat Anti-human IgM secondary antibody
Applications
Immunohistochemistry, Immunocytochemistry, Western Blot, ELISA
Synonyms
Biotin Conjugated Goat Anti-human IgM; Polyclonal Secondary Antibody; Biotin Conjugated Goat Anti-human IgM secondary antibody
Host
Goat
Clonality
Polyclonal
Isotype
IgM
Specificity
This biotin conjugated antibody is specific for human IgM and shows no cross-reactivity with human IgA/IgG.
Form/Format
Concentrated, Liquid
Concentration
0.5mg/ml (varies by lot)
Applicable Applications for Biotin Conjugated Goat Anti-human IgM secondary antibody
Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blot (WB), Direct ELISA (EIA)
Application Notes
The antibody must be used with various kinds of enzymes or fluorescence conjugated avidin (streptavidin) in IHC, ICC, WB, and ELISA.
Diluted by neutral PBS or TBS.
Recommended Dilutions:
IHC (paraffin-fixed section) - 1:100-200
IHC (frozen-fixed section) - 1:100-200
ICC - 1:100-200
WB (DAB coloration) - 1:200-400
Direct ELISA - 1:1000-2000
Optimal working dilutions must be determined by end users.
Diluted by neutral PBS or TBS.
Recommended Dilutions:
IHC (paraffin-fixed section) - 1:100-200
IHC (frozen-fixed section) - 1:100-200
ICC - 1:100-200
WB (DAB coloration) - 1:200-400
Direct ELISA - 1:1000-2000
Optimal working dilutions must be determined by end users.
Immunogen
Human IgM (whole molecular)
Contents
0.25 mg of biotin conjugated specific antibody. Biotin: Ab=20:1, 0.01M PBS, 0.01% Thimerosal, 50% Glycerol
Labeling Method
Goat anti-human IgM is conjugated biotin by means of a method described by Bayer.
(Reference: Bayer, E.A. et al., Meth.Enzymol.,62,308(1979))
(Reference: Bayer, E.A. et al., Meth.Enzymol.,62,308(1979))
Preparation and Storage
At 4 degree C for one year.
Related Product Information for Biotin Conjugated Goat Anti-human IgM secondary antibody
This antibody is purified from antiserum by immunoaffinity chromatography which removes essentially all goat serum proteins, except the specific antibody for human IgM.
Similar Products
Product Notes
The Biotin Conjugated Goat Anti-human IgM (Catalog #AAA176706) is a Secondary Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Biotin Conjugated Goat Anti-human IgM can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blot (WB), Direct ELISA (EIA). The antibody must be used with various kinds of enzymes or fluorescence conjugated avidin (streptavidin) in IHC, ICC, WB, and ELISA. Diluted by neutral PBS or TBS. Recommended Dilutions: IHC (paraffin-fixed section) - 1:100-200 IHC (frozen-fixed section) - 1:100-200 ICC - 1:100-200 WB (DAB coloration) - 1:200-400 Direct ELISA - 1:1000-2000 Optimal working dilutions must be determined by end users. Researchers should empirically determine the suitability of the Biotin Conjugated Goat Anti-human IgM for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Biotin Conjugated Goat Anti-human IgM, Polyclonal Secondary Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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