Western Blot (WB)
(Anti-HYAL2 antibody, MBS176389, Western blottingAll lanes: Anti HYAL2 (MBS176389) at 0.5ug/mlLane 1: HELA Whole Cell Lysate at 40ugLane 2: SMMC Whole Cell Lysate at 40ugLane 3: COLO320 Whole Cell Lysate at 40ugLane 4: MCF-7 Whole Cell Lysate at 40ugLane 5: HT1080 Whole Cell Lysate at 40ugPredicted bind size: 54KDObserved bind size: 54KD)
Rabbit HYAL2 Polyclonal Antibody | anti-HYAL2 antibody
Anti-HYAL2 antibody
Predicted to work with: Mouse
Predicted to work with: Mouse
Concentration: 0.1-0.5ug/ml
Tested Species: Hu, Rat
Predicted Species: Ms
Tested Species: In-house tested speceis with positive results.
Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested.
Optimal dilutions should be determined by end users.
Western Blot (WB)
(Anti-HYAL2 antibody, MBS176389, Western blottingAll lanes: Anti HYAL2 (MBS176389) at 0.5ug/mlLane 1: HELA Whole Cell Lysate at 40ugLane 2: SMMC Whole Cell Lysate at 40ugLane 3: COLO320 Whole Cell Lysate at 40ugLane 4: MCF-7 Whole Cell Lysate at 40ugLane 5: HT1080 Whole Cell Lysate at 40ugPredicted bind size: 54KDObserved bind size: 54KD)
Western Blot (WB)
(Anti-HYAL2 antibody, MBS176389, Western blottingAll lanes: Anti HYAL2 (MBS176389) at 0.5ug/mlLane 1: HELA Whole Cell Lysate at 40ugLane 2: SMMC Whole Cell Lysate at 40ugLane 3: COLO320 Whole Cell Lysate at 40ugLane 4: MCF-7 Whole Cell Lysate at 40ugLane 5: HT1080 Whole Cell Lysate at 40ugPredicted bind size: 54KDObserved bind size: 54KD)
Background: HYAL2(hyaluronoglucosaminidase 2) also known as hyaluronidase 2, LuCa-2, is an enzyme that in humans is encoded by the HYAL2 gene. HYAL2 cDNAs encode a preprotein with an N-terminal signal peptide. Northern blot analysis indicated that HYAL2 was expressed in all human tissues tested except adult brain, and Western blot analysis detected Hyal2protein in all mouse tissues examined except adult brain. The HYAL2 gene is mapped on 3p21.31. HYAL2 is a glycosylphosphatidylinositol(GPI)-anchored protein on the cell surface and serves as a receptor for entry into the cell of the jaagsiekte sheep retrovirus(JSRV). The findings of Rai et al.(2001) that HYAL2 is a GPI-anchored protein on the cell surface showed that GFP would likely be cleaved from HYAL2 during GPI addition, leaving HYAL2 on the cell surface and resulting in GFP transit to the lysosome for degradation.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a weak acid-active hyaluronidase. The encoded protein is similar in structure to other more active hyaluronidases. Hyaluronidases degrade hyaluronan, one of the major glycosaminoglycans of the extracellular matrix. Hyaluronan and fragments of hyaluronan are thought to be involved in cell proliferation, migration and differentiation. Although it was previously thought to be a lysosomal hyaluronidase that is active at a pH below 4, the encoded protein is likely a GPI-anchored cell surface protein. This hyaluronidase serves as a receptor for the oncogenic virus Jaagsiekte sheep retrovirus. The gene is one of several related genes in a region of chromosome 3p21.3 associated with tumor suppression. This gene encodes two alternatively spliced transcript variants which differ only in the 5' UTR.[provided by RefSeq, Mar 2010]
Uniprot Description
HYAL2: Hydrolyzes high molecular weight hyaluronic acid to produce an intermediate-sized product which is further hydrolyzed by sperm hyaluronidase to give small oligosaccharides. Displays very low levels of activity. Associates with and negatively regulates MST1R. Belongs to the glycosyl hydrolase 56 family.
Protein type: EC 3.2.1.35; Glycan Metabolism - glycosaminoglycan degradation; Membrane protein, GPI anchor; Hydrolase
Chromosomal Location of Human Ortholog: 3p21.3
Cellular Component: microvillus; cell surface; endoplasmic reticulum; lysosome; cytoplasmic membrane-bound vesicle; cytosol; lipid raft; Golgi membrane; anchored to external side of plasma membrane; anchored to plasma membrane; endocytic vesicle; perinuclear region of cytoplasm; apical plasma membrane; cytoplasm; plasma membrane; cytoplasmic vesicle
Molecular Function: viral receptor activity; receptor signaling protein tyrosine kinase inhibitor activity; protein binding; enzyme binding; transforming growth factor beta binding; hyaluronic acid binding; hyaluronoglucuronidase activity; receptor tyrosine kinase binding; hyalurononglucosaminidase activity
Biological Process: negative regulation of MAP kinase activity; entry of virus into host cell; negative regulation of protein kinase B signaling cascade; glycosaminoglycan metabolic process; response to virus; multicellular organismal aging; pathogenesis; hyaluronan catabolic process; response to antibiotic; response to reactive oxygen species; glycosaminoglycan catabolic process; viral envelope fusion with host membrane; monocyte activation; positive regulation of protein import into nucleus; skeletal morphogenesis; cartilage development; carbohydrate metabolic process; hemopoietic progenitor cell differentiation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of cell growth; kidney development; defense response to virus; hyaluronan metabolic process; positive regulation of inflammatory response; transformation of host cell by virus
Research Articles on HYAL2
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Product Notes
The HYAL2 hyal2 (Catalog #AAA176389) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-HYAL2 antibody reacts with Reacts with: Human, Rat Predicted to work with: Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's HYAL2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western Blot: Concentration: 0.1-0.5ug/ml Tested Species: Hu, Rat Predicted Species: Ms Tested Species: In-house tested speceis with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users. Researchers should empirically determine the suitability of the HYAL2 hyal2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HYAL2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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