Rabbit 53BP1 Polyclonal Antibody | anti-TP53BP1 antibody

Anti-53BP1 antibody

Cat. Num. AAA176333

• Gene Names: TP53BP1; p202; 53BP1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry
• Purity: Immunogen affinity purified.
• Synonyms: 53BP1; Polyclonal Antibody; Anti-53BP1 antibody; Tumor suppressor p53-binding protein 1; tumor protein p53 binding protein 1; 53 BP1 antibody; 53BP1 antibody; FLJ41424 antibody; MGC138366 antibody; p202 antibody; p53 binding protein 1 antibody; p53 BP1 antibody; p53-binding protein 1 antibody; p53BP1 antibody; TP53 BP1 antibody; TP53B_HUMAN antibody; TP53BP1 antibody; TRP53 BP1 antibody; Tumor protein 53 binding protein 1 antibody; Tumor protein p53 binding protein 1 antibody; Tumor suppressor p53 binding protein 1 antibody; Tumor suppressor p53-binding protein 1 antibody; anti-TP53BP1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized
• Sequence Length: 1972

• Applicable Applications for anti-TP53BP1 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC)
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human 53BP1 (1955-1972aa ERIGFKQHPKYKHDYVSH), identical to the related mouse sequence.
• Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti-53BP1 antibody, MBS176333, Western blottingLane 1: 293T Cell LysateLane 2: U20S Cell LysateLane 3: HEPA Cell Lysate )

Immunohistochemistry (IHC)

(Anti-53BP1 antibody, MBS176333, IHC(P)IHC(P): Human Intestinal Cancer Tissue )

Immunohistochemistry (IHC)

(Anti-53BP1 antibody, MBS176333, IHC(P)IHC(P): Rat Brain Tissue )

Immunohistochemistry (IHC)

(Anti-53BP1 antibody, MBS176333, IHC(P)IHC(P): Mouse Brain Tissue )

Immunocytochemistry (ICC)

(Anti-53BP1 antibody, MBS176333, ICCICC: A549 Cell )

Related Product Information for anti-TP53BP1 antibody

Description: Rabbit IgG polyclonal antibody for Tumor suppressor p53-binding protein 1(TP53BP1) detection. Tested with WB, IHC-P, ICC in Human, Mouse, Rat.
Background: TP53BP1(Tumor Protein p53-Binding Protein 1), also called 53BP1, is a protein that in humans is encoded by the TP53BP1 gene. Iwabuchi et al.(1998) mapped the TP53BP1 gene to 15q15-q21 by FISH. Iwabuchi et al.(1994) showed that TP53BP1 binds to the conformationally sensitive central domain of wildtype p53 but not to mutant p53 in vitro. Immunoblot analysis by Iwabuchi et al.(1998) showed that expression of TP53BP1 or TP53BP2 enhances the transactivation function of p53 and induces the expression of p21(CDKN1A). Wang et al.(2002) used small interfering RNA directed against TP53BP1 in mammalian cells to demonstrate that TP53BP1 is a key transducer of the DNA damage checkpoint signal. TP53BP1 was required for p53 accumulation, G2/M checkpoint arrest, and the intra-S-phase checkpoint in response to ionizing radiation.

NCBI and Uniprot Product Information

• NCBI GI #: 8928568
• NCBI GeneID: 7158
• UniProt Accession #: Q12888; Q2M1Z7; Q4LE46; Q5FWZ3; Q7Z3U4; F8VY86
• Molecular Weight: 213,989 Da
• NCBI Official Full Name: Tumor suppressor p53-binding protein 1
• NCBI Official Synonym Full Names: tumor protein p53 binding protein 1
• NCBI Official Symbol: TP53BP1
• NCBI Official Synonym Symbols: p202; 53BP1
• NCBI Protein Information: tumor suppressor p53-binding protein 1; p53BP1; tumor protein 53-binding protein, 1; tumor protein p53-binding protein, 1
• UniProt Protein Name: Tumor suppressor p53-binding protein 1
• Protein Family: Tumor suppressor p53-binding protein
• UniProt Gene Name: TP53BP1
• UniProt Synonym Gene Names: 53BP1; p53-binding protein 1; p53BP1
• UniProt Entry Name: TP53B_HUMAN

Uniprot Description

53BP1: a DNA damage checkpoint protein that interacts with HDAC4 protein to mediate the DNA damage response. Inhibits E2F1-mediated apoptosis in prostate cancer cells. Recruited to double strand breaks via the binding of its Tudor domain to dimethylated H3 K20. Asymmetrically dimethylated on Arg residues by PRMT1. Methylation is required for DNA binding. Binds to the central domain of p53. Interacts with Artemis. Interacts with histone H2AFX and this requires phosphorylation of H2AFX on S139. Interacts with histone H4 that has been dimethylated at K20. Has low affinity for histone H4 containing monomethylated K20. Does not bind histone H4 containing unmethylated or trimethylated K20. Has low affinity for histone H3 that has been dimethylated on K79. Does not bind unmethylated histone H3. Phosphorylated at basal level in the absence of DNA damage. Hyper-phosphorylated in an ATM-dependent manner in response to DNA damage induced by ionizing radiation. Hyper-phosphorylated in an ATR-dependent manner in response to DNA damage induced by UV irradiation. Two alternatively spliced human isoforms have been observed.

Protein type: Transcription, coactivator/corepressor; DNA repair, damage

Chromosomal Location of Human Ortholog: 15q15-q21

Cellular Component: nucleoplasm; chromosome, telomeric region; cytoplasm; replication fork; nucleus

Molecular Function: protein binding; p53 binding; damaged DNA binding; telomeric DNA binding; methylated histone residue binding

Biological Process: transcription from RNA polymerase II promoter; double-strand break repair; positive regulation of transcription, DNA-dependent; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; DNA repair; response to DNA damage stimulus; double-strand break repair via homologous recombination