Rabbit anti-Human PKR Polyclonal Antibody | anti-EIF2AK2 antibody

Anti-PKR antibody

Cat. Num. AAA176094

• Gene Names: EIF2AK2; PKR; PRKR; EIF2AK1; PPP1R83
• Reactivity: Human
• Applications: Western Blot
• Purity: Immunogen affinity purified.
• Synonyms: PKR; Polyclonal Antibody; Anti-PKR antibody; Interferon-induced; double-stranded RNA-activated protein kinase; eukaryotic translation initiation factor 2-alpha kinase 2; double stranded RNA activated protein kinase; antibody; E2AK2_HUMAN antibody; eIF-2A protein kinase 2 antibody; EIF2AK1 antibody; EIF2AK2 antibody; Eukaryotic translation initiation factor 2 alpha kinase 2 antibody; Eukaryotic translation initiation factor 2-alpha kinase 2 antibody; eukaryotic translation initiation factor 2-alpha kinase antibody; HGNC:9437 antibody; Interferon induced double stranded RNA activated protein kinase antibody; Interferon inducible elF2 alpha kinase antibody; Interferon inducible RNA dependent protein kinase antibody; Interferon inducible RNA-dependent protein kinase antibody; double-stranded RNA-activated protein kinase antibody; Interferon-inducible RNA-dependent protein kinase antibody; MGC126524 antibody; P1/eIF-2A protein kinase antibody; P1/eIF2A protein kinase antibody; p68 kinase antibody; PKR antibody; PRKR antibody; Protein kinase interferon inducible double stranded RNA dependent antibody; Protein kinase RNA activated antibody; Protein kinase RNA-activated antibody; Serine/threonine protein kinase TIK antibody; Serine/threonine-protein kinase TIK antibody; Tyrosine protein kinase EIF2AK2 antibody; anti-EIF2AK2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized
• Sequence Length: 551

• Applicable Applications for anti-EIF2AK2 antibody: Western Blot (WB)
• Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human PKR (2-18aa AGDLSAGFFMEELNTYR).
• Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti-PKR antibody, MBS176094, Western blottingRecombinant Protein Detection Source: E.coli derived -recombinant Human EIF2AK2, 35.0KD(162aa tag+ M1-K154)Lane 1: Recombinant Human EIF2AK2 Protein 10ngLane 2: Recombinant Human EIF2AK2 Protein 5ngLane 3: Recombinant Human EIF2AK2 Protein 2.5ngLane 4: Recombinant Human EIF2AK2 Protein 1.25ng )

Related Product Information for anti-EIF2AK2 antibody

Description: Rabbit IgG polyclonal antibody for Interferon-induced, double-stranded RNA-activated protein kinase(EIF2AK2) detection. Tested with WB in Human.
Background: EIF2AK2(Eukaryotic Translation Initiation Factor 2-Alpha Kinase 2), also called PKR, is an enzyme that in humans is encoded by the EIF2AK2 gene. Activation of EIF2AK2 allows the kinase to phosphorylate its natural substrate, the alpha subunit of eukaryotic protein synthesis initiation factor-2, leading to the inhibition of protein synthesis(summary by Kuhen et al., 1996). By FISH analysis, Squire et al.(1993) assigned the EIF2AK2 gene to the boundary between chromosome 2p22-p21. Ben-Asouli et al.(2002) showed that human gamma-interferon mRNA uses local activation of PKR in the cell to control its own translation yield. IFNG mRNA was found to activate PKR through a pseudoknot in its 5-prime untranslated region. Taylor et al.(1999) studied the mechanism underlying the resistance of hepatitis C virus(HCV) to interferon. They demonstrated that the HCV envelope protein E2 contains a sequence identical with phosphorylation sites of the interferon-inducible protein kinase PKR and the translation initiation factor EIF2-alpha, a target of PKR. E2 inhibited the kinase activity of PKR and blocked its inhibitory effect on protein synthesis and cell growth.

NCBI and Uniprot Product Information

• NCBI GI #: 125527
• NCBI GeneID: 5610
• UniProt Accession #: P19525; Q52M43; Q7Z6F6; Q9UIR4; A8K3P0; D6W584; E9PC80
• Molecular Weight: 57,391 Da
• NCBI Official Full Name: Interferon-induced, double-stranded RNA-activated protein kinase
• NCBI Official Synonym Full Names: eukaryotic translation initiation factor 2-alpha kinase 2
• NCBI Official Symbol: EIF2AK2
• NCBI Official Synonym Symbols: PKR; PRKR; EIF2AK1; PPP1R83
• NCBI Protein Information: interferon-induced, double-stranded RNA-activated protein kinase; p68 kinase; eIF-2A protein kinase 2; P1/eIF-2A protein kinase; tyrosine-protein kinase EIF2AK2; interferon-inducible elF2alpha kinase; double stranded RNA activated protein kinase; protein phosphatase 1, regulatory subunit 83; protein kinase, interferon-inducible double stranded RNA dependent
• UniProt Protein Name: Interferon-induced, double-stranded RNA-activated protein kinase
• Protein Family: Interferon-induced, double-stranded RNA-activated protein kinase
• UniProt Gene Name: EIF2AK2
• UniProt Synonym Gene Names: PKR; PRKR; eIF-2A protein kinase 2; PKR
• UniProt Entry Name: E2AK2_HUMAN

NCBI Description

The protein encoded by this gene is a serine/threonine protein kinase that is activated by autophosphorylation after binding to dsRNA. The activated form of the encoded protein can phosphorylate translation initiation factor EIF2S1, which in turn inhibits protein synthesis. This protein is also activated by manganese ions and heparin. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

Uniprot Description

PKR: a protein kinase of the PEK family. Upon binding double-stranded RNA, it becomes autophosphorylated and activated. Phosphorylates and inhibits the alpha subunit of eIF2 alpha, which leads to an inhibition of the initiation of protein synthesis. Controls the activation of several transcription factors such as NF-kappaB, p53 and Stats. Mediates apoptosis induced by many different stimuli, such as LPS, TNF-alpha, viral infection and serum starvation.

Protein type: Protein kinase, Other; EC 2.7.10.2; EC 2.7.11.1; Translation; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Other group; PEK family

Chromosomal Location of Human Ortholog: 2p22-p21

Cellular Component: membrane; perinuclear region of cytoplasm; cytoplasm; ribosome; cytosol; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; double-stranded RNA binding; non-membrane spanning protein tyrosine kinase activity; protein phosphatase type 2A regulator activity; ATP binding; eukaryotic translation initiation factor 2alpha kinase activity; protein kinase activity

Biological Process: positive regulation of cytokine production; peptidyl-tyrosine phosphorylation; transcription, DNA-dependent; translation; activation of MAPKK activity; unfolded protein response; protein amino acid autophosphorylation; response to virus; viral infectious cycle; protein amino acid phosphorylation; evasion by virus of host immune response; positive regulation of stress-activated MAPK cascade; positive regulation of chemokine production; activation of NF-kappaB transcription factor; negative regulation of cell proliferation; modification by virus of host cellular process; negative regulation of viral genome replication; virus-host interaction; negative regulation of translation; innate immune response; negative regulation of osteoblast proliferation; defense response to virus; negative regulation of apoptosis