Mouse RBP4 ELISA Kit | RBP4 elisa kit

Mouse RBP4 PicoKine ELISA Kit

Cat. Num. AAA175911

• Gene Names: Rbp4; Rbp-4
• Reactivity: Mouse
• Synonyms: RBP4; Mouse RBP4 PicoKine ELISA Kit; Retinol-binding protein 4; retinol binding protein 4; plasma; OTTHUMP00000020114; OTTHUMP00000020115; OTTHUMP00000020116; Plasma retinol binding protein 4; Plasma retinol-binding protein; Plasma retinol-binding protein(1-176); PRBP; PRO2222; RBP; RDCCAS; RET4_HUMAN; Retinol binding protein 4; Retinol binding protein 4 interstitial; Retinol binding protein 4 plasma; RBP4 elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant mouse RBP4
• Sequence Length: 245

• Samples: Cell culture supernates, serum, plasma (heparin, EDTA) and urine
• Assay Type: Sandwich
• Detection Range: 312pg/ml-20,000pg/ml
• Sensitivity: < 10pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Mouse RBP4 PicoKine ELISA Kit standard curve )

Related Product Information for RBP4 elisa kit

Principle of the assay: mouse RBP4 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for RBP4 has been precoated onto 96-well plates. Standards(NSO, E19-L201) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for RBP4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse RBP4 amount of sample captured in plate.
Background: Retinol binding protein 4, plasma, also known as RBP4, belongs to the lipocalin family and is the specific carrier for retinol (vitamin A alcohol) in the blood. It is protein that it is encoded by the RBP4 gene. RBP4 gene resides just centromeric of the cluster of CYP2C genes on 10q24. The mouse RBP4 locus is closely linked and just proximal to the locus for phenobarbital-inducible cytochrome P450-2c (Cyp-2c) at the distal end of chromosome 19. It delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin, which prevents its loss by filtration through the kidney glomeruli. A deficiency of vitamin A blocks secretion of the binding protein posttranslationally and results in defective delivery and supply to the epidermal cells. The standard product used in this kit are recombinant mouse B7-1/CD80, D37—K245, consisting of dimer acids with two single straned. The standard used in this kit is recombinant protein, with E19-L201 aa sequence, the molecular weight is 22kda.

NCBI and Uniprot Product Information

• NCBI GeneID: 19662
• UniProt Accession #: H7BWY6; Q00724
• Molecular Weight: 28,405 Da
• NCBI Official Synonym Full Names: retinol binding protein 4, plasma
• NCBI Official Symbol: Rbp4
• NCBI Official Synonym Symbols: Rbp-4
• NCBI Protein Information: retinol-binding protein 4; RBP; PRBP; plasma retinol-binding protein; retinol binding protein 4, cellular
• UniProt Protein Name: Retinol-binding protein 4
• Protein Family: Retinol-binding protein
• UniProt Gene Name: Rbp4
• UniProt Entry Name: H7BWY6_MOUSE

Uniprot Description

RBP4: Delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin, this prevents its loss by filtration through the kidney glomeruli. Defects in RBP4 are a cause of retinol-binding protein deficiency (RBP deficiency). This condition causes night vision problems. It produces a typical 'fundus xerophthalmicus', featuring a progressed atrophy of the retinal pigment epithelium. Belongs to the calycin superfamily. Lipocalin family.

Protein type: Secreted, signal peptide; Secreted

Cellular Component: extracellular space; protein complex; extracellular region

Molecular Function: retinoid binding; protein binding; transporter activity; protein heterodimerization activity; retinal binding; retinol binding

Biological Process: retinal metabolic process; embryonic skeletal development; maintenance of gastrointestinal epithelium; heart development; glucose homeostasis; uterus development; response to insulin stimulus; embryonic organ morphogenesis; retinol metabolic process; transport; negative regulation of cardiac muscle cell proliferation; detection of light stimulus involved in visual perception; female genitalia morphogenesis; cardiac muscle development; response to retinoic acid; urinary bladder development; male gonad development; vagina development; positive regulation of insulin secretion; positive regulation of immunoglobulin secretion; vitamin transport; gluconeogenesis; embryonic development; eye development; retina development in camera-type eye; embryonic retina morphogenesis in camera-type eye; spermatogenesis; lung development