Mouse CXCL1 ELISA Kit | CXCL1 elisa kit

Mouse CXCL1 PicoKine ELISA Kit

Cat. Num. AAA175855

• Gene Names: Cxcl1; KC; Fsp; N51; gro; Gro1; Mgsa; Scyb1
• Reactivity: Mouse
• Synonyms: CXCL1; Mouse CXCL1 PicoKine ELISA Kit; C-X-C motif chemokine 1; chemokine (C-X-C motif) ligand 1; chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity; alpha); fibroblast secretory protein; FSP; GRO; GRO1; GRO1 oncogene (melanoma growth stimulating activity; GRO1 oncogene (melanoma growth-stimulating activity); GROa; growth-regulated alpha protein; Melanoma growth stimulatory activity; melanoma growth stimulatory activity alpha; MGSA; MGSA a; MGSA alpha; NAP 3; Neutrophil-activating protein 3; SCYB1; CXCL1 elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant mouse CXCL1
• Sequence Length: 96

• Samples: Cell culture supernates and serum
• Assay Type: Sandwich
• Detection Range: 15.6pg/ml-1000pg/ml
• Sensitivity: < 1pg/ml
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Mouse CXCL1 PicoKine ELISA Kit standard curve )

Related Product Information for CXCL1 elisa kit

Principle of the assay: mouse CXCL1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CXCL1 has been precoated onto 96-well plates. Standards(E. coli, R20-K96) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CXCL1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CXCL1 amount of sample captured in plate.
Background: Chemokine (C-X-C motif) ligand 1 (CXCL1) is a small cytokine belonging to the CXC chemokine family that was previously called GRO1 oncogene, GROalpha, KC, Neutrophil-activating protein 3 (NAP-3) and melanoma growth stimulating activity, alpha (MSGA-alpha). In humans, this protein is encoded by the CXCL1 gene. The gene for CXCL1 is located on human chromosome 4 amongst genes for other CXC chemokines. The mature form of CXCL1 is maximally 73 amino acids long. CXCL1 is secreted by human melanoma cells, has mitogenic properties and is implicated in melanoma pathogenesis. CXCL1 is expressed by macrophages, neutrophils and epithelial cells, and has neutrophil chemoattractant activity. This chemokine elicits its effects by signaling through the chemokine receptor CXCR2.CXCL1 decreased the severity of multiple sclerosis and may offer a neuro-protective function. The standard product used in this kit is recombinant mouse CXCL1, consisting of 77 amino acids with the molecular mass of 8KDa.

NCBI and Uniprot Product Information

• NCBI GeneID: 14825
• UniProt Accession #: A2RTH0; P12850
• Molecular Weight: 10,254 Da
• NCBI Official Synonym Full Names: chemokine (C-X-C motif) ligand 1
• NCBI Official Symbol: Cxcl1
• NCBI Official Synonym Symbols: KC; Fsp; N51; gro; Gro1; Mgsa; Scyb1
• NCBI Protein Information: growth-regulated alpha protein; KC/GR)-alpha; KC/GRO-alpha; GRO1 oncogene; alpha-chemokine; secretory protein N51; C-X-C motif chemokine 1; platelet-derived growth factor-inducible protein KC
• UniProt Protein Name: C-X-C motif chemokine
• Protein Family: Growth-regulated alpha protein
• UniProt Gene Name: Cxcl1
• UniProt Entry Name: A2RTH0_MOUSE

NCBI Description

This gene encodes a protein that is a member of the CXC subfamily of chemokines. Chemokines, which recruit and activate leukocytes, are classified by function (inflammatory or homeostatic) or by structure. This secretory protein is proposed to bind the G-protein coupled receptor chemokine (C-X-C motif) receptor 2 to recruit neutrophils. In mouse, deficiency of this gene is associated with colitis and with defects in immune cell recruitment to the lung. [provided by RefSeq, Apr 2013]

Uniprot Description

CXCL2: Produced by activated monocytes and neutrophils and expressed at sites of inflammation. Hematoregulatory chemokine, which, in vitro, suppresses hematopoietic progenitor cell proliferation. GRO-beta(5-73) shows a highly enhanced hematopoietic activity. Belongs to the intercrine alpha (chemokine CxC) family.

Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Cellular Component: extracellular space; cell; extracellular region; cytosol

Molecular Function: growth factor activity; chemokine activity; CXCR chemokine receptor binding; cytokine activity

Biological Process: neutrophil chemotaxis; G-protein coupled receptor protein signaling pathway; elevation of cytosolic calcium ion concentration; response to molecule of bacterial origin; positive regulation of superoxide release; positive regulation of potassium ion transport; immune response; response to lipopolysaccharide; positive regulation of leukocyte chemotaxis; inflammatory response; acute inflammatory response; regulation of cell proliferation