Typical Testing Data/Standard Curve (for reference only)
(Rat IL-1 alpha PicoKine ELISA Kit standard curve )
Rat IL-1 alpha ELISA Kit | IL1A elisa kit
Rat IL-1 alpha PicoKine ELISA Kit
Gene Names
Il1a; IL-1 alpha
Reactivity
Rat
Synonyms
IL-1 alpha; Rat IL-1 alpha PicoKine ELISA Kit; Interleukin-1 alpha; interleukin 1; alpha; Hematopoietin 1; IFL; IFN; IFN alpha; IFN-alpha-1/13; IFNA@; IFNA1; IFNA1_HUMAN; IFNA13; IFNAP22; IL 1 alpha; Il 1a; IL1; IL1 ALPHA; IL1A; IL1F1; Ilia; Interferon Alpha; Interferon alpha 1; Interferon alpha 1/13; Interferon alpha 1/13 precursor; Interferon alpha 13; Interferon alpha D; Interferon alpha leukocyte; Interferon alpha-1/13; Interferon alpha-D; Interferon leukocyte; Interleukin 1 alpha; Interleukin 1 alpha precursor; Interleukin1 alpha; LeIF D; MGC138207; MGC138505; MGC138507; OTTHUMP00000203692; OTTMUSP00000016430; Preinterleukin 1; Pro interleukin 1; Pro interleukin 1 alpha; Prointerleukin 1; RP23-160G19.8; IL1A elisa kit
Reactivity
Rat
Specificity
Natural and recombinant rat IL-1alpha
Sequence Length
270
Samples
Cell culture supernates and serum
Assay Type
Sandwich
Detection Range
4.69pg/ml-300pg/ml
Sensitivity
< 0.5pg/ml
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Typical Testing Data/Standard Curve (for reference only)
(Rat IL-1 alpha PicoKine ELISA Kit standard curve )
Typical Testing Data/Standard Curve (for reference only)
(Rat IL-1 alpha PicoKine ELISA Kit standard curve )
Related Product Information for IL1A elisa kit
Principle of the assay: Rat IL-1alpha ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-1alpha has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-1alpha is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat IL-1alpha amount of sample captured in plate.
Background: Interleukin-1 alpha (IL-1 alpha) and interleukin-1 beta (IL-1 beta) are two biochemically distinct, but distantly related, polypeptidic cytokines that play a key role in inflammation, immunologic reactions, and tissue repair.1 IL-1alpha has been implicated in the pathogenesis of infectious, autoimmune and inflammatory diseases.2 Recently, ithas been shown that IL-1 alpha is identical to hematopoietin 1, which is described as a hematopoietic growth factor acting on early progenitor cells in synergy with other hematopoietic growth factors.1 The human interleukin 1alpha gene is assigned to chromosome 2.3 Genetic polymorphisms at interleukin (IL)-1alpha and IL-1beta havebeen recently suggested to be associated with severity of adult periodontitis.4 The murine IL-1 alpha and IL-1 betagenes encode structurally and evolutionarily related cytokines that exert a regulatory role in numerous physiological processes including hemopoiesis.5 The standard product used in this kit is recombinant rat IL-1alpha withthe molecular mass of 18kDa.
Background: Interleukin-1 alpha (IL-1 alpha) and interleukin-1 beta (IL-1 beta) are two biochemically distinct, but distantly related, polypeptidic cytokines that play a key role in inflammation, immunologic reactions, and tissue repair.1 IL-1alpha has been implicated in the pathogenesis of infectious, autoimmune and inflammatory diseases.2 Recently, ithas been shown that IL-1 alpha is identical to hematopoietin 1, which is described as a hematopoietic growth factor acting on early progenitor cells in synergy with other hematopoietic growth factors.1 The human interleukin 1alpha gene is assigned to chromosome 2.3 Genetic polymorphisms at interleukin (IL)-1alpha and IL-1beta havebeen recently suggested to be associated with severity of adult periodontitis.4 The murine IL-1 alpha and IL-1 betagenes encode structurally and evolutionarily related cytokines that exert a regulatory role in numerous physiological processes including hemopoiesis.5 The standard product used in this kit is recombinant rat IL-1alpha withthe molecular mass of 18kDa.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
30,856 Da
NCBI Official Full Name
Interleukin-1 alpha
NCBI Official Synonym Full Names
interleukin 1 alpha
NCBI Official Symbol
Il1a
NCBI Official Synonym Symbols
IL-1 alpha
NCBI Protein Information
interleukin-1 alpha
UniProt Protein Name
Interleukin-1 alpha
Protein Family
UniProt Gene Name
Il1a
UniProt Synonym Gene Names
IL-1 alpha
UniProt Entry Name
IL1A_RAT
NCBI Description
proinflammatory cytokine [RGD, Feb 2006]
Uniprot Description
Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
Research Articles on IL1A
Similar Products
Product Notes
The Rat IL1A il1a (Catalog #AAA175842) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA175842 ELISA Kit recognizes Rat IL1A. It is sometimes possible for the material contained within the vial of "IL-1 alpha, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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