Loading...

Skip to main content

Call us on + 1 (800) 604-9114 for more information about our products

Looking for specific datasheet Manual/COA/MSDS?
Request a Manual/COA/MSDS

Interested to get a quote about our products?
Request a Quote

Western Blot (WB) (Anti-MCM3 antibody, MBS175507, Western blottingAll lanes: Anti MCM3 (MBS175507) at 0.5ug/mlLane 1: NIH3T3 Whole Cell Lysate at 40ugLane 2: HEPA Whole Cell Lysate at 40ugPredicted bind size: 91KDObserved bind size: 91KD )

Rabbit MCM3 Polyclonal Antibody | anti-MCM3 antibody

Anti-MCM3 antibody

Gene Names
MCM3; HCC5; P1.h; RLFB; P1-MCM3
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry
Purity
Immunogen affinity purified.
Synonyms
MCM3; Polyclonal Antibody; Anti-MCM3 antibody; DNA replication licensing factor MCM3; minichromosome maintenance complex component 3; Cervical cancer proto oncogene 5 antibody; DNA polymerase alpha holoenzyme associated P1 antibody; DNA polymerase alpha holoenzyme associated protein P1 antibody; DNA polymerase alpha holoenzyme-associated protein P1 antibody; DNA replication factor MCM3 antibody; DNA replication licensing factor MCM3 antibody; HCC 5 antibody; HCC5 antibody; hRlf beta subunit antibody; Human cervical cancer proto oncogene 5 antibody; MCM 3 antibody; mcm3 antibody; MCM3 minichromosome maintenance deficient 3 antibody; MCM3_HUMAN antibody; MGC1157 antibody; Minichromosome maintenance complex component 3 antibody; Minichromosome maintenance deficient 3 antibody; Minichromosome maintenance protein 3 antibody; P1 h antibody; P1 MCM3 antibody; P1 Protein antibody; P1-MCM3 antibody; P1.h antibody; p102 antibody; P102 protein antibody; Replication licensing factor beta subunit antibody; RLF beta subunit antibody; RLF subunit beta antibody; RLFB antibody; anti-MCM3 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Sequence Length
808
Applicable Applications for anti-MCM3 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC)
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human MCM3 (13-27aa REAQRDYLDFLDDEE), identical to the related rat and mouse sequences.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti-MCM3 antibody, MBS175507, Western blottingAll lanes: Anti MCM3 (MBS175507) at 0.5ug/mlLane 1: NIH3T3 Whole Cell Lysate at 40ugLane 2: HEPA Whole Cell Lysate at 40ugPredicted bind size: 91KDObserved bind size: 91KD )

Western Blot (WB) (Anti-MCM3 antibody, MBS175507, Western blottingAll lanes: Anti MCM3 (MBS175507) at 0.5ug/mlLane 1: NIH3T3 Whole Cell Lysate at 40ugLane 2: HEPA Whole Cell Lysate at 40ugPredicted bind size: 91KDObserved bind size: 91KD )

Immunohistochemistry (IHC)

(Anti-MCM3 antibody, MBS175507, IHC(P)IHC(P): Human Lung Cancer Tissue )

Immunohistochemistry (IHC) (Anti-MCM3 antibody, MBS175507, IHC(P)IHC(P): Human Lung Cancer Tissue )

Immunohistochemistry (IHC)

(Anti-MCM3 antibody, MBS175507, IHC(P)IHC(P): Rat Intestine Tissue )

Immunohistochemistry (IHC) (Anti-MCM3 antibody, MBS175507, IHC(P)IHC(P): Rat Intestine Tissue )

Immunohistochemistry (IHC)

(Anti-MCM3 antibody, MBS175507, IHC(P)IHC(P): Rat Intestine Tissue )

Immunohistochemistry (IHC) (Anti-MCM3 antibody, MBS175507, IHC(P)IHC(P): Rat Intestine Tissue )

Immunohistochemistry (IHC)

(Anti-MCM3 antibody, MBS175507, IHC(P)IHC(P): Mouse Intestine Tissue )

Immunohistochemistry (IHC) (Anti-MCM3 antibody, MBS175507, IHC(P)IHC(P): Mouse Intestine Tissue )

Immunocytochemistry (ICC)

(Anti-MCM3 antibody, MBS175507, ICCICC: HELA Cell )

Immunocytochemistry (ICC) (Anti-MCM3 antibody, MBS175507, ICCICC: HELA Cell )
Related Product Information for anti-MCM3 antibody
Description: Rabbit IgG polyclonal antibody for DNA replication licensing factor MCM3(MCM3) detection. Tested with WB, IHC-P, ICC in Human, Mouse, Rat.
Background: MCM3(MINICHROMOSOME MAINTENANCE, S. CEREVISIAE, HOMOLOG OF, 3), also called RLFB or P1 PROTEIN, is a protein that in humans is encoded by the MCM3 gene. MCM3 is one of the highly conserved mini-chromosome maintenance proteins(MCM) that are involved in the initiation of eukaryotic genome replication. The MCM3 gene is mapped to 6p12.2. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with, and thus is acetlyated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
95,908 Da
NCBI Official Full Name
DNA replication licensing factor MCM3
NCBI Official Synonym Full Names
minichromosome maintenance complex component 3
NCBI Official Symbol
MCM3
NCBI Official Synonym Symbols
HCC5; P1.h; RLFB; P1-MCM3
NCBI Protein Information
DNA replication licensing factor MCM3; p102; RLF subunit beta; hRlf beta subunit; DNA replication factor MCM3; cervical cancer proto-oncogene 5; minichromosome maintenance deficient 3; replication licensing factor, beta subunit; MCM3 minichromosome maintenance deficient 3; DNA polymerase alpha holoenzyme-associated protein P1
UniProt Protein Name
DNA replication licensing factor MCM3
UniProt Gene Name
MCM3
UniProt Entry Name
MCM3_HUMAN

NCBI Description

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with and is acetylated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2012]

Uniprot Description

MCM3: a mini-chromosome maintenance protein, essential for the initiation of eukaryotic genome replication. Allows DNA to undergo a single round of replication per cell cycle. Required for DNA replication and cell proliferation.

Protein type: DNA replication; EC 3.6.4.12

Chromosomal Location of Human Ortholog: 6p12

Cellular Component: nucleoplasm; centrosome; alpha DNA polymerase:primase complex; intracellular membrane-bound organelle; MCM complex; membrane; perinuclear region of cytoplasm; nucleus

Molecular Function: protein binding; DNA helicase activity; DNA binding; ATP binding

Biological Process: DNA replication initiation; mitotic cell cycle; DNA strand elongation during DNA replication; DNA replication; DNA duplex unwinding; G1/S transition of mitotic cell cycle

Research Articles on MCM3

Similar Products

Product Notes

The MCM3 mcm3 (Catalog #AAA175507) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-MCM3 antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MCM3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC). Researchers should empirically determine the suitability of the MCM3 mcm3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MCM3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

Item has been added to Shopping Cart

If you are ready to order, navigate to Shopping Cart and get ready to checkout.

Looking for a specific manual?
Request a Manual

Request more Information

Please complete the form below and a representative will contact you as soon as possible.

Request a Manual

Please complete the form below and a representative will contact you as soon as possible.

Request a Quote

Please complete the form below and a representative will contact you as soon as possible.