Rabbit anti-Human FGF21 Polyclonal Antibody | anti-FGF21 antibody

Anti-FGF21 antibody

Cat. Num. AAA175449

• Reactivity: Human
• Applications: Western Blot
• Purity: Immunogen affinity purified.
• Synonyms: FGF21; Polyclonal Antibody; Anti-FGF21 antibody; Fibroblast growth factor 21(FGF-21); fibroblast growth factor 21; FGF 21 antibody; FGF-21 antibody; Fgf21 antibody; FGF21_HUMAN antibody; FGFL antibody; Fibroblast growth factor 21 antibody; PRO10196 antibody; UNQ3115 antibody; anti-FGF21 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: No cross reactivity with other proteins
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized
• Sequence Length: 209

• Applicable Applications for anti-FGF21 antibody: Western Blot (WB)
• Application Notes: Western Blot:; Concentration: 0.1-0.5ug/ml; Tested Species: Human; Tested Species: In-house tested species with positive results; Predicted Species: Species predicted to be fit for the product based on sequence similarities.; ; Other applications have not been tested. Optimal dilutions should be determined by end user.
• Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human FGF21 (43-58aa QVRQRYLYTDDAQQTE), different from the related rat and mouse sequences by two amino acids.
• Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti-FGF21 antibody, MBS175449, Western blottingAll lanes: Anti FGF21 (MBS175449) at 0.5ug/mlLane 1: A549 Whole Cell Lysate at 40ugLane 2: A431 Whole Cell Lysate at 40ugLane 3: HEPA Whole Cell Lysate at 40ugPredicted bind size: 21KDObserved bind size: 21KD)

Related Product Information for anti-FGF21 antibody

FGF21(Fibroblast growth factor 21) is a protein that in humans is encoded by the FGF21 gene, which is also a member of the fibroblast growth factor(FGF) family. The FGF21 gene is mapped on 19q13.33. Using RT-PCR, Fgf21 was expressed in several types of adipose tissue in mice, including subcutaneous and epididymal fat pads and brown adipose tissue. The level of Fgf21 expression in adipose tissue was comparable to that in liver. FGF21 stimulates glucose uptake in adipocytes but not in other cell types. This effect is additive to the activity of insulin. FGF21 treatment of adipocytes is associated with phosphorylation of FRS2, a protein linking FGF receptors to the Ras/MAP kinase pathway. FGF21 also protects animals from diet-induced obesity when overexpressed in transgenic mice and lowers blood glucose and triglyceride levels when administered to diabetic rodents. Changes in Fgf21 expression due to suckling or nutritional manipulations were associated with changes in circulating free fatty acid and ketone body levels. In differentiated mouse brown adipocytes in culture, Fgf21 treatment increased the expression of thermogenic genes, caused higher total and uncoupled respiration, and enhanced glucose oxidation.

NCBI and Uniprot Product Information

• NCBI GI #: 13626703
• NCBI GeneID: 26291
• UniProt Accession #: Q9NSA1; Q8N683
• Molecular Weight: 22,300 Da
• NCBI Official Full Name: Fibroblast growth factor 21
• NCBI Official Synonym Full Names: fibroblast growth factor 21
• NCBI Official Symbol: FGF21
• NCBI Protein Information: fibroblast growth factor 21
• UniProt Protein Name: Fibroblast growth factor 21
• Protein Family: Fibroblast growth factor
• UniProt Gene Name: FGF21
• UniProt Synonym Gene Names: FGF-21
• UniProt Entry Name: FGF21_HUMAN

NCBI Description

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. The function of this growth factor has not yet been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

FGF21: Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity requires the presence of KLB. Belongs to the heparin-binding growth factors family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 19q13.33

Cellular Component: extracellular region

Molecular Function: protein binding; growth factor activity; fibroblast growth factor receptor binding

Biological Process: positive regulation of glucose import; cell-cell signaling; positive regulation of cell proliferation; positive regulation of low-density lipoprotein receptor biosynthetic process; signal transduction