Rabbit anti-Rat HIF-2 alpha Polyclonal Antibody | anti-HIF-2 alpha antibody

Polyclonal Anti-HIF-2 alpha

Cat. Num. AAA175439

• Gene Names: HIF3A; IPAS; MOP7; PASD7; HIF-3A; bHLHe17
• Reactivity: Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen affinity purified.
• Synonyms: HIF-2 alpha; Polyclonal Antibody; Polyclonal Anti-HIF-2 alpha; Polyclonal Anti-HIF-2a; anti-HIF-2 alpha antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized
• Sequence Length: 669

• Applicable Applications for anti-HIF-2 alpha antibody: Western Blot (WB), Immunohistochemistry (IHC)/Paraffin
• Immunogen: A synthetic peptide corresponding to a sequence at N-termianl of rat HIF- 2alpha.
• Reconstitution: 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20?C for a longer time. Avoid repeated freezing and thawing.

Testing Data

Western Blot

Immunohistochemistry

Related Product Information for anti-HIF-2 alpha antibody

HIF-2 alpha is also designated EPAS1 whose gene is mapped to 2p21-p16. The predicted mouse protein is 88% identical to human EPAS1. The human EPAS1 gene contains 15 exons and spans at least 120 kb. The positions of the introns within the genomic region encoding the N-terminal bHLH-PAS domains of EPAS1 and AHR are similar, suggesting that the 5-prime ends of the 2 genes may have arisen from a gene duplication event1. Moreover, the predicted protein shares 48% sequence identity with HIF1-alpha, a bHLH-PAS transcription factor that induces EPO gene expression in cultured cells in response to hypoxia. Like HIF1A, EPAS1 binds to and activates transcription from the HIF1A response element derived from the 3-prime flanking region of the EPO gene. EPAS1 is predominantly expressed in highly vascularized tissues of adult humans and in endothelial cells of the mouse adult and embryo. Furthermore, EPAS1 may represent an important regulator of vascularization, perhaps involving the regulation of endothelial cell gene expression in response to hypoxia2. HIF2A is expressed at relatively higher levels in villus sections of placenta and in lung samples compared with other tissues examined3. In addition, The variation in EPAS1 influences the relative contribution of aerobic and anaerobic metabolism and hence the maximum sustainable metabolic power for a given event duration4.

References

1. Tian, H.; McKnight, S. L.; Russell, D. W. : Endothelial PAS domain protein 1 (EPAS1), a transcription factor selectively expressed in endothelial cells. Genes Dev. 11: 72-82, 1997.
2. Sood, R.; Zehnder, J. L.; Druzin, M. L.; Brown, P. O. : Gene expression patterns in human placenta. Proc. Nat. Acad. Sci. 103: 5478-5483, 2006.
3. Henderson, J.; Withford-Cave, J. M.; Duffy, D. L.; Cole, S. J.; Sawyer, N. A.; Gulbin, J. P.; Hahn, A.; Trent, R. J.; Yu, B. : The EPAS1 gene influences the aerobic-anaerobic contribution in elite endurance athletes. Hum. Genet. 118: 416-423, 2005

NCBI and Uniprot Product Information

• NCBI GI #: 90421311
• NCBI GeneID: 64344
• NCBI Accession #: NP_690008.2
• NCBI GenBank Nucleotide #: NM_152795.3
• UniProt Accession #: Q9Y2N7; Q58A43; Q66K72; Q8WXA1; Q96K34; Q9H7Z9; Q9HAI2; B0M185; B4DNA2
• Molecular Weight: 72,433 Da
• NCBI Official Full Name: hypoxia-inducible factor 3-alpha isoform c
• NCBI Official Synonym Full Names: hypoxia inducible factor 3, alpha subunit
• NCBI Official Symbol: HIF3A
• NCBI Official Synonym Symbols: IPAS; MOP7; PASD7; HIF-3A; bHLHe17
• NCBI Protein Information: hypoxia-inducible factor 3-alpha; HIF3-alpha-1; member of PAS protein 7; inhibitory PAS domain protein; PAS domain-containing protein 7; basic-helix-loop-helix-PAS protein MOP7; class E basic helix-loop-helix protein 17
• UniProt Protein Name: Hypoxia-inducible factor 3-alpha
• UniProt Gene Name: HIF3A
• UniProt Synonym Gene Names: BHLHE17; MOP7; PASD7; HIF-3-alpha; HIF3-alpha; bHLHe17; IPAS
• UniProt Entry Name: HIF3A_HUMAN

NCBI Description

The protein encoded by this gene is the alpha-3 subunit of one of several alpha/beta-subunit heterodimeric transcription factors that regulate many adaptive responses to low oxygen tension (hypoxia). The alpha-3 subunit lacks the transactivation domain found in factors containing either the alpha-1 or alpha-2 subunits. It is thought that factors containing the alpha-3 subunit are negative regulators of hypoxia-inducible gene expression. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2011]

Uniprot Description

HIF3A: Involved in adaptive response to hypoxia. Suppresses hypoxia-inducible expression of HIF1A and EPAS1. Binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters. The complex HIF3A-ARNT activates the transcription of reporter genes driven by HRE. Isoform 4 has a dominant-negative function of inactivating HIF1A-mediated transcription. Isoform 4 attenuates the binding of HIF1A to hypoxia-responsive elements (HRE), thus inhibiting HRE-driven transcription. Hypoxia induces down-regulation of isoform 4, leading to activation of HIF1A in hypoxia. Conversely, upon restoring normoxia, the expression of isoform 4 increases and thereby secure an inhibition of HIF1A activity. Isoform 4 may be a negative regulator of hypoxia-inducible gene expression in the kidney and may be involved in renal tumorigenesis. Functions as an inhibitor of angiogenesis in the cornea. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Tumor suppressor; Transcription factor

Chromosomal Location of Human Ortholog: 19q13.32

Cellular Component: nucleoplasm; cytosol

Molecular Function: protein dimerization activity; signal transducer activity; DNA binding; transcription coactivator activity; transcription factor activity; transcription corepressor activity

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter; signal transduction