Chemical Structure
(Chemical structure)
Ponceau S solution Reagent
Ponceau S solution
Applications
Western Blot
Synonyms
Ponceau S solution; Ponceau S solution reagent
Form/Format
Liquid
Concentration
0.1 % (w/v) in 5% acetic acid
Working Concentration: for PVDF and nitrocellulose membrane (varies by lot)
Working Concentration: for PVDF and nitrocellulose membrane (varies by lot)
Applicable Applications for Ponceau S solution reagent
Western Blot (WB)
Application Notes
Ponceau S solution has been used for membrane staining (Western blotting)
Molecular formula
C22H12N4Na4O13S4
CAS number
6226-79-5
Protocol
1. Immerse the PVDF or nitrocellulose membrane in the Ponceau S solution working solution. Shake for 3-5 min or for a longer time until clear bands appear.
2. Rinse the membrane in distilled water, PBS buffer or other appropriate solution 2-3 times, for 3-5 minutes each.
2. Rinse the membrane in distilled water, PBS buffer or other appropriate solution 2-3 times, for 3-5 minutes each.
Preparation and Storage
Upon receipt store at 4 degree C. It is stable at 4 degree C for one year.
Chemical Structure
(Chemical structure)
Chemical Structure
(Chemical structure)
Testing Data
Testing Data
Related Product Information for Ponceau S solution reagent
Introduction: Ponceau S solution can be used for rapid staining of protein bands on PVDF and nitrocellulose membrane. Since the dye of this reagent is with negative charge, it can combine with amino-acid residue with positive charge. Meanwhile, it also can combine with non-polar area of protein to form red bands thereby. For the reversible protein staining, protein can be cleaned again by washing with distilled water, PBS buffer or other appropriate solution. With advantages of convenient use, low background and high sensitivity, this product can detect protein at minimum 250ng, but it is not suitable for protein detection on nylon membrane.
Product Categories/Family for Ponceau S solution reagent
Similar Products
Product Notes
The Ponceau S solution (Catalog #AAA1753874) is a Reagent and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Ponceau S solution can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Ponceau S solution has been used for membrane staining (Western blotting). Researchers should empirically determine the suitability of the Ponceau S solution for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ponceau S solution, Reagent" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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