Western Blot (WB)
(Figure 1. Western blot analysis of ZWINT using anti-ZWINT antibody (MBS1753753).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: rat thymus tissue lysatesLane 2: rat liver tissue lysatesLane 3: rat testis tissue lysatesLane 4: rat RH35 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-ZWINT antigen affinity purified polyclonal antibody (Catalog # MBS1753753) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for ZWINT at approximately 38KD. The expected band size for ZWINT is at 38KD. )
Rabbit anti-Rat ZWINT Polyclonal Antibody | anti-Zwint antibody
Anti-ZWINT Antibody
Direct ELISA: 0.1-0.5ug/ml|Rat|
Western Blot (WB)
(Figure 1. Western blot analysis of ZWINT using anti-ZWINT antibody (MBS1753753).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: rat thymus tissue lysatesLane 2: rat liver tissue lysatesLane 3: rat testis tissue lysatesLane 4: rat RH35 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-ZWINT antigen affinity purified polyclonal antibody (Catalog # MBS1753753) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for ZWINT at approximately 38KD. The expected band size for ZWINT is at 38KD. )
Western Blot (WB)
(Figure 1. Western blot analysis of ZWINT using anti-ZWINT antibody (MBS1753753).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: rat thymus tissue lysatesLane 2: rat liver tissue lysatesLane 3: rat testis tissue lysatesLane 4: rat RH35 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-ZWINT antigen affinity purified polyclonal antibody (Catalog # MBS1753753) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for ZWINT at approximately 38KD. The expected band size for ZWINT is at 38KD. )
2. Obuse C, Iwasaki O, Kiyomitsu T, Goshima G, Toyoda Y, Yanagida M (Nov 2004). "A conserved Mis12 centromere complex is linked to heterochromatic HP1 and outer kinetochore protein Zwint-1". Nature Cell Biology 6 (11): 1135-41.
3. van Vlijmen T, Vleugel M, Evers M, Mohammed S, Wulf PS, Heck AJ, Hoogenraad CC, van der Sluijs P (Aug 2008). "A unique residue in rab3c determines the interaction with novel binding protein Zwint-1". FEBS Letters 582 (19): 2838-42.
NCBI and Uniprot Product Information
NCBI Description
directly interacts with SNAP25; may be involved in exocytosis of synaptic vesicles in neurotransmission [RGD, Feb 2006]
Uniprot Description
ZWINT: Part of the MIS12 complex, which is required for kinetochore formation and spindle checkpoint activity. Required to target ZW10 to the kinetochore at prometaphase.
Protein type: Cell cycle regulation
Cellular Component: kinetochore; neuron projection; intracellular membrane-bound organelle; dendrite; cytoplasm; nucleus
Molecular Function: protein binding; protein N-terminus binding
Biological Process: mitotic sister chromatid segregation; cell division; mitotic cell cycle checkpoint; establishment of cellular localization
Research Articles on Zwint
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Product Notes
The Zwint zwint (Catalog #AAA1753753) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-ZWINT Antibody reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ZWINT can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Direct ELISA (EIA). WB: 0.25-0.5ug/ml|Rat| Direct ELISA: 0.1-0.5ug/ml|Rat|. Researchers should empirically determine the suitability of the Zwint zwint for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ZWINT, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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